The JI PBL Intereron Source
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     
 

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Baars, P. A.
Right arrow Articles by van Lier, R. A. W.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Baars, P. A.
Right arrow Articles by van Lier, R. A. W.
The Journal of Immunology, 00, 165: 1910-1917.
Copyright © 00 by The American Association of Immunologists

Cytolytic Mechanisms and Expression of Activation-Regulating Receptors on Effector-Type CD8+CD45RA+CD27- Human T Cells

Paul A. Baars1,*, Laura M. Ribeiro do Couto*,1,2,3, Jeanette H. W. Leusen{dagger}, Berend Hooibrink*, Taco W. Kuijpers*, Susanne M. A. Lens4,* and René A. W. van Lier5,*

* Department of Immunobiology and Laboratory for Experimental and Clinical Immunology, Academic Medical Center, CLB, University of Amsterdam, Amsterdam, The Netherlands; and {dagger} Department of Cell Biology, University Medical Center Utrecht, Utrecht, The Netherlands

Circulating CD8+ T cells with a CD45RA+CD27- phenotype resemble cytolytic effector cells because they express various cytolytic mediators and are able to execute cytotoxicity without prior stimulation in vitro. We here demonstrate that CD8+CD45RA+CD27- T cells can use both granule exocytosis and Fas/Fas ligand pathways to induce apoptosis in target cells. The availability of these cytolytic mechanisms in circulating T cells suggests that the activity of these cells must be carefully controlled to prevent unwanted tissue damage. For this reason, we analyzed the expression of surface receptors that either enhance or inhibit T cell function. Compared with memory-type cells, effector cells were found to express normal levels of CD3{epsilon} and TCR{zeta} and relatively high levels of CD8. CTLA-4 was absent from freshly isolated effector cells, whereas a limited number of unstimulated memory cells expressed this molecule. In line with recent findings on CD8+CD28- T cells, CD45RA+CD27- T cells were unique in the abundant expression of NK cell-inhibitory receptors, both of Ig superfamily and C-type lectin classes. Binding of NK cell-inhibitory receptors to classical and nonclassical MHC class I molecules may inhibit the activation of the cytolytic machinery induced by either Ag receptor-specific or nonspecific signals in CD8+CD45RA+CD27- T cells.




This article has been cited by other articles:


Home page
 J. Immunol.Home page
G. D. Basu, T. L. Tinder, J. M. Bradley, T. Tu, C. L. Hattrup, B. A. Pockaj, and P. Mukherjee
Cyclooxygenase-2 Inhibitor Enhances the Efficacy of a Breast Cancer Vaccine: Role of IDO
J. Immunol., August 15, 2006; 177(4): 2391 - 2402.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
N. Rufer, A. Zippelius, P. Batard, M. J. Pittet, I. Kurth, P. Corthesy, J.-C. Cerottini, S. Leyvraz, E. Roosnek, M. Nabholz, et al.
Ex vivo characterization of human CD8+ T subsets with distinct replicative history and partial effector functions
Blood, September 1, 2003; 102(5): 1779 - 1787.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
W. J. M. Mackus, F. N. J. Frakking, A. Grummels, L. E. Gamadia, G. J. de Bree, D. Hamann, R. A. W. van Lier, and M. H. J. van Oers
Expansion of CMV-specific CD8+CD45RA+CD27- T cells in B-cell chronic lymphocytic leukemia
Blood, August 1, 2003; 102(3): 1057 - 1063.
[Abstract] [Full Text] [PDF]

Home page
 Arch DermatolHome page
M. Lebwohl, E. Christophers, R. Langley, J. P. Ortonne, J. Roberts, and C. E. M. Griffiths
An International, Randomized, Double-blind, Placebo-Controlled Phase 3 Trial of Intramuscular Alefacept in Patients With Chronic Plaque Psoriasis
Arch Dermatol, June 1, 2003; 139(6): 719 - 727.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
T. W. Kuijpers, M. T. Vossen, M.-R. Gent, J.-C. Davin, M. T. Roos, P. M. Wertheim-van Dillen, J. F. Weel, P. A. Baars, and R. A. van Lier
Frequencies of Circulating Cytolytic, CD45RA+CD27-, CD8+ T Lymphocytes Depend on Infection with CMV
J. Immunol., April 15, 2003; 170(8): 4342 - 4348.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
E. M. van Leeuwen, L. E. Gamadia, P. A. Baars, E. B. Remmerswaal, I. J. ten Berge, and R. A. van Lier
Proliferation Requirements of Cytomegalovirus-Specific, Effector-Type Human CD8+ T Cells
J. Immunol., November 15, 2002; 169(10): 5838 - 5843.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
Y.-J. Kim, R. R. Brutkiewicz, and H. E. Broxmeyer
Role of 4-1BB (CD137) in the functional activation of cord blood CD28-CD8+ T cells
Blood, October 16, 2002; 100(9): 3253 - 3260.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
Y.-J. Chwae, M. J. Chang, S. M. Park, H. Yoon, H.-J. Park, S. J. Kim, and J. Kim
Molecular Mechanism of the Activation-Induced Cell Death Inhibition Mediated by a p70 Inhibitory Killer Cell Ig-Like Receptor in Jurkat T Cells
J. Immunol., October 1, 2002; 169(7): 3726 - 3735.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Pathol.Home page
Y. Mizukawa, Y. Yamazaki, Y. Teraki, J. Hayakawa, K. Hayakawa, H. Nuriya, M. Kohara, and T. Shiohara
Direct Evidence for Interferon-{gamma} Production by Effector-Memory-Type Intraepidermal T Cells Residing at an Effector Site of Immunopathology in Fixed Drug Eruption
Am. J. Pathol., October 1, 2002; 161(4): 1337 - 1347.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
H. Wedemeyer, X.-S. He, M. Nascimbeni, A. R. Davis, H. B. Greenberg, J. H. Hoofnagle, T. J. Liang, H. Alter, and B. Rehermann
Impaired Effector Function of Hepatitis C Virus-Specific CD8+ T Cells in Chronic Hepatitis C Virus Infection
J. Immunol., September 15, 2002; 169(6): 3447 - 3458.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
A. Suarez, L. Mozo, and C. Gutierrez
Generation of CD4+CD45RA+ Effector T Cells by Stimulation in the Presence of Cyclic Adenosine 5'-Monophosphate- Elevating Agents
J. Immunol., August 1, 2002; 169(3): 1159 - 1167.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
V. Monsurro, D. Nagorsen, E. Wang, M. Provenzano, M. E. Dudley, S. A. Rosenberg, and F. M. Marincola
Functional Heterogeneity of Vaccine-Induced CD8+ T Cells
J. Immunol., June 1, 2002; 168(11): 5933 - 5942.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
A. D. Hislop, N. H. Gudgeon, M. F. C. Callan, C. Fazou, H. Hasegawa, M. Salmon, and A. B. Rickinson
EBV-Specific CD8+ T Cell Memory: Relationships Between Epitope Specificity, Cell Phenotype, and Immediate Effector Function
J. Immunol., August 15, 2001; 167(4): 2019 - 2029.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
L. E. Gamadia, R. J. Rentenaar, P. A. Baars, E. B. M. Remmerswaal, S. Surachno, J. F. L. Weel, M. Toebes, T. N. M. Schumacher, I. J. M. ten Berge, and R. A. W. van Lier
Differentiation of cytomegalovirus-specific CD8+ T cells in healthy and immunosuppressed virus carriers
Blood, August 1, 2001; 98(3): 754 - 761.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
G. Chen, P. Shankar, C. Lange, H. Valdez, P. R. Skolnik, L. Wu, N. Manjunath, and J. Lieberman
CD8 T cells specific for human immunodeficiency virus, Epstein-Barr virus, and cytomegalovirus lack molecules for homing to lymphoid sites of infection
Blood, July 1, 2001; 98(1): 156 - 164.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Website Copyright © 2000 by The American Association of Immunologists, Inc. All rights reserved.
All Contents Copyright © 2000 by The American Association of Immunologists, Inc. All rights reserved.