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inkora2,3,*inkora3,*
*
Department of Immunology and Gnotobiology, Institute of Microbiology, Czech Academy of Sciences, Nov
Hrádek, Czech Republic; and
Department of Microbiology, University of Iowa, Iowa City, IA 52242
Successive colonization of the thymus by waves of thymocyte
progenitors has been described in chicken-quail chimeras and suggested
from studies in mice. In swine, we show that the first CD3
-bearing
thymocytes appear on day 40 of gestation (DG40). These early thymocytes
were CD3high and belonged to the 
T cell lineage.
Mature CD3high 
ß thymocytes were observed 15 days
later (DG55), and their occurrence was preceded by the appearance of
CD3low thymocytes (DG45). Thereafter, we observed
transient changes in thymocyte subset composition (DG56-DG74), which
can be explained by a gap in pro-T cell delivery to the thymus. This
delivery gap corresponds with the expression of the pan-leukocyte CD45
and pan-myelomonocytic SWC3a markers in fetal liver and bone marrow and
is probably caused by shifting of primary lymphopoiesis between these
organs. Therefore, we conclude that the embryonic thymus is colonized
by at least two successive waves of hemopoietic progenitors during
embryogenesis and that the influx of thymocyte progenitors is
discontinuous. Surface immunophenotyping and cell cycle analysis of
thymocyte subsets allowed us to compare thymocyte differentiation in
pigs with that described for rodents and humans and to propose a model
for T cell lymphopoiesis in swine. We also observed that the porcine
IL-2R (CD25), a typical differentiation marker of pre-T cells in
mice and humans, was not expressed on thymocyte precursors in pigs and
could only be found on mature thymocytes. Finally, we observed a subset
of TCR+ thymocytes that were cycling late during
their development in the thymus.
This article has been cited by other articles:
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  J. E. Butler, C. D. Lemke, P. Weber, M. Sinkora, and K. M. Lager Antibody Repertoire Development in Fetal and Neonatal Piglets: XIX. Undiversified B Cells with Hydrophobic HCDR3s Preferentially Proliferate in the Porcine Reproductive and Respiratory Syndrome J. Immunol., May 15, 2007; 178(10): 6320 - 6331. [Abstract] [Full Text] [PDF]
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M. Sinkora, J. Sinkorova, Z. Cimburek, and W. Holtmeier Two Groups of Porcine TCR{gamma}{delta}+ Thymocytes Behave and Diverge Differently J. Immunol., January 15, 2007; 178(2): 711 - 719. [Abstract] [Full Text] [PDF]
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M. Sinkora, J. Sun, J. Sinkorova, R. K. Christenson, S. P. Ford, and J. E. Butler Antibody Repertoire Development in Fetal and Neonatal Piglets. VI. B Cell Lymphogenesis Occurs at Multiple Sites with Differences in the Frequency of In-frame Rearrangements J. Immunol., February 15, 2003; 170(4): 1781 - 1788. [Abstract] [Full Text] [PDF]
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J. E. Butler, P. Weber, M. Sinkora, J. Sun, S. J. Ford, and R. K. Christenson Antibody Repertoire Development in Fetal and Neonatal Piglets. II. Characterization of Heavy Chain Complementarity-Determining Region 3 Diversity in the Developing Fetus J. Immunol., December 15, 2000; 165(12): 6999 - 7010. [Abstract] [Full Text] [PDF]
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