| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
*
Medical Research Council Molecular Haematology Unit, Institute of Molecular Medicine, John Radcliffe Hospital, Headington, Oxford, United Kingdom;
Hanson Centre for Cancer Research, Adelaide, Australia;
Stem Cell Laboratory, Peter MacCallum Cancer Institute, Melbourne, Australia; and
§
Medizinische Universitätsklinik II, University of Tubingen, Tubingen, Germany
The novel sialomucin, CD164, functions as both an adhesion receptor on human CD34+ cell subsets in bone marrow and as a potent negative regulator of CD34+ hemopoietic progenitor cell proliferation. These diverse effects are mediated by at least two functional epitopes defined by the mAbs, 103B2/9E10 and 105A5. We report here the precise epitope mapping of these mAbs together with that of two other CD164 mAbs, N6B6 and 67D2. Using newly defined CD164 splice variants and a set of soluble recombinant chimeric proteins encoded by exons 1–6 of the CD164 gene, we demonstrate that the 105A5 and 103B2/9E10 functional epitopes map to distinct glycosylated regions within the first mucin domain of CD164. The N6B6 and 67D2 mAbs, in contrast, recognize closely associated and complex epitopes that rely on the conformational integrity of the CD164 molecule and encompass the cysteine-rich regions encoded by exons 2 and 3. On the basis of their sensitivities to reducing agents and to sialidase, O-sialoglycoprotease, and N-glycanase treatments, we have characterized CD164 epitopes and grouped them into three classes by analogy with CD34 epitope classification. The class I 105A5 epitope is sialidase, O-glycosidase, and O-sialoglycoprotease sensitive; the class II 103B2/9E10 epitope is N-glycanase, O-glycosidase, and O-sialoglycoprotease sensitive; and the class III N6B6 and 67D2 epitopes are not removed by such enzyme treatments. Collectively, this study indicates that the previously observed differential expression of CD164 epitopes in adult tissues is linked with cell type specific post-translational modifications and suggests a role for epitope-associated carbohydrate structures in CD164 function.
This article has been cited by other articles:
|
|
 |
|
  E. Martin-Rendon, S. J.M. Hale, D. Ryan, D. Baban, S. P. Forde, M. Roubelakis, D. Sweeney, M. Moukayed, A. L. Harris, K. Davies, et al. Transcriptional Profiling of Human Cord Blood CD133+ and Cultured Bone Marrow Mesenchymal Stem Cells in Response to Hypoxia Stem Cells, April 1, 2007; 25(4): 1003 - 1012. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Forde, B. J. Tye, S. E. Newey, M. Roubelakis, J. Smythe, C. P. McGuckin, R. Pettengell, and S. M. Watt Endolyn (CD164) modulates the CXCL12-mediated migration of umbilical cord blood CD133+ cells Blood, March 1, 2007; 109(5): 1825 - 1833. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Serafini, S. J. Dylla, M. Oki, Y. Heremans, J. Tolar, Y. Jiang, S. M. Buckley, B. Pelacho, T. C. Burns, S. Frommer, et al. Hematopoietic reconstitution by multipotent adult progenitor cells: precursors to long-term hematopoietic stem cells J. Exp. Med., January 22, 2007; 204(1): 129 - 139. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. A. Potter, G. Ihrke, J. R. Bruns, K. M. Weixel, and O. A. Weisz Specific N-Glycans Direct Apical Delivery of Transmembrane, but Not Soluble or Glycosylphosphatidylinositol-anchored Forms of Endolyn in Madin-Darby Canine Kidney Cells Mol. Biol. Cell, March 1, 2004; 15(3): 1407 - 1416. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. P. McGuckin, N. Forraz, M.-O. Baradez, C. Lojo-Rial, D. Wertheim, K. Whiting, S. M. Watt, and R. Pettengell Colocalization Analysis of Sialomucins CD34 and CD164 Stem Cells, March 1, 2003; 21(2): 162 - 170. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 U. Samulowitz, A. Kuhn, G. Brachtendorf, R. Nawroth, A. Braun, A. Bankfalvi, W. Bocker, and D. Vestweber Human Endomucin : Distribution Pattern, Expression on High Endothelial Venules, and Decoration with the MECA-79 Epitope Am. J. Pathol., May 1, 2002; 160(5): 1669 - 1681. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y.-N. Lee, J.-S. Kang, and R. S. Krauss Identification of a Role for the Sialomucin CD164 in Myogenic Differentiation by Signal Sequence Trapping in Yeast Mol. Cell. Biol., November 15, 2001; 21(22): 7696 - 7706. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Prosper and C. M. Verfaillie Regulation of hematopoiesis through adhesion receptors J. Leukoc. Biol., March 1, 2001; 69(3): 307 - 316. [Abstract] [Full Text]
|
|
|
|
 |
|
 J. Y.-H. Chan, J. E. Lee-Prudhoe, B. Jorgensen, G. Ihrke, R. Doyonnas, A. C. W. Zannettino, V. J. Buckle, C. J. Ward, P. J. Simmons, and S. M. Watt Relationship between Novel Isoforms, Functionally Important Domains, and Subcellular Distribution of CD164/Endolyn J. Biol. Chem., January 12, 2001; 276(3): 2139 - 2152. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
