HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by De Creus, A. Articles by Leclercq, G. Search for Related Content PubMed PubMed Citation Articles by De Creus, A. Articles by Leclercq, G.

Langerhans Cells That Have Matured In Vivo in the Absence of T Cells Are Fully Capable of Inducing a Helper CD4 as Well as a Cytotoxic CD8 Response¹

Department of Clinical Chemistry, Microbiology, and Immunology, Ghent University Hospital, Ghent, Belgium

Langerhans cells (LCs) are immature dendritic cells (DCs) present in the skin epithelium. Upon Ag exposure, they migrate to the draining lymph nodes where they mature into potent stimulators of naive T cells. The aim of this study was to investigate the influence of T cells on LC migration and maturation. Therefore, the in vivo migration and maturation of LCs after sensitization with the hapten FITC was compared between C57BL/6 or BALB/c mice used as positive controls, and recombination activating gene (RAG) 1 knockout (^-/-) mice or SCID mice used as T cell-deficient mice. Phenotypically, there was no difference between migrated LCs from RAG1^-/- or SCID mice vs normal C57BL/6 or BALB/c mice: both populations of FITC⁺ cells had a dendritic morphology and a mature phenotype as they expressed high levels of MHC class II molecules and costimulatory molecules CD80, CD86, and CD54. Sorted migrated LCs of RAG1^-/- or SCID mice were efficient stimulators of allogeneic T cells and Ag-specific CD4⁺ T cells. The same results were found if migrated LCs were fixed instead of irradiated, excluding the possibility that LCs derived from RAG1^-/- or SCID mice would mature in the presence of T cells during the stimulation tests. Importantly, fixed migrated LCs of RAG1^-/- mice were also efficient stimulators of cytotoxic CD8⁺ T cells. These data suggest that T cells are not required for full maturation of LCs.

This article has been cited by other articles:

				N. Brewig, A. Kissenpfennig, B. Malissen, A. Veit, T. Bickert, B. Fleischer, S. Mostbock, and U. Ritter Priming of CD8+ and CD4+ T Cells in Experimental Leishmaniasis Is Initiated by Different Dendritic Cell Subtypes J. Immunol., January 15, 2009; 182(2): 774 - 783. [Abstract] [Full Text] [PDF]

				L. de Repentigny, D. Lewandowski, and P. Jolicoeur Immunopathogenesis of Oropharyngeal Candidiasis in Human Immunodeficiency Virus Infection Clin. Microbiol. Rev., October 1, 2004; 17(4): 729 - 759. [Abstract] [Full Text] [PDF]

				P. A. Dullforce, K. L. Garman, G. W. Seitz, R. J. Fleischmann, S. M. Crespo, S. R. Planck, D. C. Parker, and J. T. Rosenbaum APCs in the Anterior Uveal Tract Do Not Migrate to Draining Lymph Nodes J. Immunol., June 1, 2004; 172(11): 6701 - 6708. [Abstract] [Full Text] [PDF]

				J. M. Hernandez, M. H. T. Bui, K.-r. Han, H. Mukouyama, D. G. Freitas, D. Nguyen, R. Caliliw, P. I. Shintaku, S. H. Paik, C.-L. Tso, et al. Novel Kidney Cancer Immunotherapy Based on the Granulocyte- Macrophage Colony-stimulating Factor and Carbonic Anhydrase IX Fusion Gene Clin. Cancer Res., May 1, 2003; 9(5): 1906 - 1916. [Abstract] [Full Text] [PDF]

				A. Rizzitelli, R. Berthier, V. Collin, S. M. Candeias, and P. N. Marche T Lymphocytes Potentiate Murine Dendritic Cells to Produce IL-12 J. Immunol., October 15, 2002; 169(8): 4237 - 4245. [Abstract] [Full Text] [PDF]

				A. De Creus, K. Van Beneden, F. Stevenaert, V. Debacker, J. Plum, and G. Leclercq Developmental and Functional Defects of Thymic and Epidermal V{gamma}3 Cells in IL-15-Deficient and IFN Regulatory Factor-1-Deficient Mice J. Immunol., June 15, 2002; 168(12): 6486 - 6493. [Abstract] [Full Text] [PDF]

				Y. Belkaid, E. Von Stebut, S. Mendez, R. Lira, E. Caler, S. Bertholet, M. C. Udey, and D. Sacks CD8+ T Cells Are Required for Primary Immunity in C57BL/6 Mice Following Low-Dose, Intradermal Challenge with Leishmania major J. Immunol., April 15, 2002; 168(8): 3992 - 4000. [Abstract] [Full Text] [PDF]

				J. Li, B. Schuler-Thurner, G. Schuler, C. Huber, and B. Seliger Bipartite regulation of different components of the MHC class I antigen-processing machinery during dendritic cell maturation Int. Immunol., December 1, 2001; 13(12): 1515 - 1523. [Abstract] [Full Text] [PDF]