HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Sültmann, H. Articles by Klein, J. Search for Related Content PubMed PubMed Citation Articles by Sültmann, H. Articles by Klein, J.

Conservation of Mhc Class III Region Synteny Between Zebrafish and Human as Determined by Radiation Hybrid Mapping¹

Holger Sültmann^2,*, Akie Sato^*, Brent W. Murray^*, Naoko Takezaki, Robert Geisler, Gerd-Jörg Rauch and Jan Klein^3,*

^* Max-Planck-Institut für Biologie, Abteilung Immungenetik, Tübingen, Germany; The Graduate University for Advanced Studies, Department of Biosystems Science, Hayama, Kanagawa, Japan; and Max-Planck-Institut für Entwicklungsbiologie, Tübingen, Germany

In the HLA, H2, and other mammalian Mhc, the class I and II loci are separated by the so-called class III region comprised of 60 genes that are functionally and evolutionarily unrelated to the class I/II genes. To explore the origin of this island of unrelated loci in the middle of the Mhc 19 homologues of HLA class III genes, we identified 19 homologues of HLA class III genes as well as 21 additional non-class I/II HLA homologues in the zebrafish and mapped them by testing a panel of 94 zebrafish-hamster radiation hybrid cell lines. Six of the HLA class III and eight of the flanking homologues were found to be linked to the zebrafish class I (but not class II) loci in linkage group 19. The remaining homologous loci were found to be scattered over 14 zebrafish linkage groups. The linkage group 19 contains at least 25 genes (not counting the class I loci) that are also syntenic on human chromosome 6. This gene assembly presumably represents the pre-Mhc that existed before the class I/II genes arose. The pre-Mhc may not have contained the complement and other class III genes involved in immune response.

This article has been cited by other articles:

				Y. Ohta, W. Goetz, M. Z. Hossain, M. Nonaka, and M. F. Flajnik Ancestral Organization of the MHC Revealed in the Amphibian Xenopus J. Immunol., March 15, 2006; 176(6): 3674 - 3685. [Abstract] [Full Text] [PDF]

				J. M. Spitsbergen and M. L. Kent The State of the Art of the Zebrafish Model for Toxicology and Toxicologic Pathology Research--Advantages and Current Limitations Toxicol Pathol, January 1, 2003; 31(1_suppl): 62 - 87. [Abstract] [PDF]

				C. P. Kruiswijk, T. T. Hermsen, A. H. Westphal, H. F. J. Savelkoul, and R. J. M. Stet A Novel Functional Class I Lineage in Zebrafish (Danio rerio), Carp (Cyprinus carpio), and Large Barbus (Barbus intermedius) Showing an Unusual Conservation of the Peptide Binding Domains J. Immunol., August 15, 2002; 169(4): 1936 - 1947. [Abstract] [Full Text] [PDF]