The JI
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     
 

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Jeon, M.-S.
Right arrow Articles by Esser, C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Jeon, M.-S.
Right arrow Articles by Esser, C.
Right arrowPubmed/NCBI databases
*Compound via MeSH
*Substance via MeSH
Hazardous Substances DB
*2,3,7,8-TETRACHLORODIBENZO-P-DIOXIN
The Journal of Immunology, 2000, 165: 6975-6983.
Copyright © 2000 by The American Association of Immunologists

The Murine IL-2 Promoter Contains Distal Regulatory Elements Responsive to the Ah Receptor, a Member of the Evolutionarily Conserved bHLH-PAS Transcription Factor Family1

Myung-Shin Jeon and Charlotte Esser2

Division of Immunology, Medical Institute of Environmental Hygiene at the University of Düsseldorf, Düsseldorf, Germany

Signaling through the TCR and costimulatory signals primarily control transcription of the IL-2 gene in naive T cells. The minimal promoter necessary for this expression lies proximal, between -300 and the transcription start site. We had previously shown that activation of the arylhydrocarbon receptor (AHR), a member of the bHLH-PAS family of transcription factors, leads to increased mRNA expression of IL-2 in murine fetal thymocytes. The AHR is abundant in the thymus and may play a role for the development of the immune system. Moreover, its overactivation by chemicals such as dioxins leads to immunosuppression and thymic involution. Binding motifs for the liganded AHR can be identified in the distal region -1300 to -800 of the mouse IL-2 promoter. We show here that these DNA motifs, the so-called dioxin response elements, after binding to the liganded AHR are sufficient to transactivate luciferase expression in a reporter gene system. The IL-2 gene can be induced by the AHR also in thymocytes in vivo after injection of 2,3,7,8-tetrachlorodibenzo-p-dioxin, a potent ligand of the AHR. The AHR mediates the IL-2 induction as shown with AHR-deficient mice. However, in spleen cells in vitro costimulation via the TCR is necessary for optimal IL-2 gene induction. Thus, the IL-2 promoter region contains novel distal regulatory elements that can be addressed by the AHR to induce IL-2 and can cooperate with the proximal promoter in this.




This article has been cited by other articles:


Home page
 J. Immunol.Home page
N. B. Marshall, W. R. Vorachek, L. B. Steppan, D. V. Mourich, and N. I. Kerkvliet
Functional Characterization and Gene Expression Analysis of CD4+CD25+ Regulatory T Cells Generated in Mice Treated with 2,3,7,8-Tetrachlorodibenzo-p-Dioxin
J. Immunol., August 15, 2008; 181(4): 2382 - 2391.
[Abstract] [Full Text] [PDF]

Home page
 Am J Trop Med HygHome page
O. J. BOWERS, K. B. SOMMERSTED, R. T. SOWELL, G. E. BOLING, W. H. HANNEMAN, R. G. TITUS, and G. K. DEKREY
2,3,7,8-TETRACHLORODIBENZO-P-DIOXIN (TCDD) REDUCES LEISHMANIA MAJOR BURDENS IN C57BL/6 MICE.
Am J Trop Med Hyg, October 1, 2006; 75(4): 749 - 752.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. N'Diaye, E. Le Ferrec, D. Lagadic-Gossmann, S. Corre, D. Gilot, V. Lecureur, P. Monteiro, C. Rauch, M.-D. Galibert, and O. Fardel
Aryl Hydrocarbon Receptor- and Calcium-dependent Induction of the Chemokine CCL1 by the Environmental Contaminant Benzo[a]pyrene
J. Biol. Chem., July 21, 2006; 281(29): 19906 - 19915.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
C. J. Funatake, N. B. Marshall, L. B. Steppan, D. V. Mourich, and N. I. Kerkvliet
Cutting Edge: Activation of the Aryl Hydrocarbon Receptor by 2,3,7,8-Tetrachlorodibenzo-p-dioxin Generates a Population of CD4+CD25+ Cells with Characteristics of Regulatory T Cells
J. Immunol., October 1, 2005; 175(7): 4184 - 4188.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Pharmacol.Home page
M. T. Fisher, M. Nagarkatti, and P. S. Nagarkatti
2,3,7,8-Tetrachlorodibenzo-p-dioxin Enhances Negative Selection of T Cells in the Thymus but Allows Autoreactive T Cells to Escape Deletion and Migrate to the Periphery
Mol. Pharmacol., January 1, 2005; 67(1): 327 - 335.
[Abstract] [Full Text] [PDF]

Home page
 Toxicol SciHome page
J. Adachi, Y. Mori, S. Matsui, and T. Matsuda
Comparison of Gene Expression Patterns between 2,3,7,8-Tetrachlorodibenzo-p-dioxin and a Natural Arylhydrocarbon Receptor Ligand, Indirubin
Toxicol. Sci., July 1, 2004; 80(1): 161 - 169.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
C. Yang, F. Boucher, A. Tremblay, and J. L. Michaud
Regulatory Interaction between Arylhydrocarbon Receptor and SIM1, Two Basic Helix-Loop-Helix PAS Proteins Involved in the Control of Food Intake
J. Biol. Chem., March 5, 2004; 279(10): 9306 - 9312.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
S. Tomita, H.-B. Jiang, T. Ueno, S. Takagi, K. Tohi, S.-i. Maekawa, A. Miyatake, A. Furukawa, F. J. Gonzalez, J. Takeda, et al.
T Cell-Specific Disruption of Arylhydrocarbon Receptor Nuclear Translocator (Arnt) Gene Causes Resistance to 2,3,7,8-Tetrachlorodibenzo-p-dioxin-Induced Thymic Involution
J. Immunol., October 15, 2003; 171(8): 4113 - 4120.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Website Copyright © 2000 by The American Association of Immunologists, Inc. All rights reserved.
All Contents Copyright © 2000 by The American Association of Immunologists, Inc. All rights reserved.