| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
*
Naval Medical Research Institute, Bethesda, MD 20814;
Genetics Institute, Cambridge, MA 02140; and
Department of Molecular and Cellular Engineering, University of Pennsylvania, Philadelphia, PA 19104
To examine the role of CD28 and CTLA-4 in Th cell differentiation, we used a novel microsphere-based system to compare the effects of CD28 ligation by Ab or CD80/CD86. One set of beads was prepared by coating with anti-CD3 and anti-CD28 Ab. Another set of beads was prepared by immobilizing anti-CD3 and murine CD80-Ig fusion protein or murine CD86-Ig fusion protein on the beads. The three sets of beads were compared in their effects on the ability to activate and differentiate splenic CD4 T cells. When purified naive CD4+ cells were stimulated in vitro, robust proliferation of similar magnitude was induced by all three sets of beads. When cytokine secretion was examined, all bead preparations induced an equivalent accumulation of IL-2. In contrast, there was a marked difference in the cytokine secretion pattern of the Th2 cytokines IL-4, IL-10, and IL-13. The B7-Ig-stimulated cultures had high concentrations of Th2 cytokines, whereas there were low or undetectable concentrations in the anti-CD28-stimulated cultures. Addition of anti-CTLA-4 Fab augmented B7-mediated IL-4 secretion. These studies demonstrate that B7 is a critical and potent stimulator of Th2 differentiation, and that anti-CD28 prevents this effect.
This article has been cited by other articles:
|
|
 |
|
  T. Guichelaar, C. B. ten Brink, P. J. van Kooten, S. E. Berlo, C. P. Broeren, W. van Eden, and F. Broere Autoantigen-Specific IL-10-Transduced T Cells Suppress Chronic Arthritis by Promoting the Endogenous Regulatory IL-10 Response J. Immunol., February 1, 2008; 180(3): 1373 - 1381. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. B. Graham, M. P. Bell, C. J. Huntoon, M. D. Griffin, X. Tai, A. Singer, and D. J. McKean CD28 Ligation Costimulates Cell Death but Not Maturation of Double-Positive Thymocytes due to Defective ERK MAPK Signaling J. Immunol., November 1, 2006; 177(9): 6098 - 6107. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Caron, L. Lariviere, M. Nacache, M. Tam, M. M. Stevenson, C. McKerly, P. Gros, and D. Malo Influence of Slc11a1 on the Outcome of Salmonella enterica Serovar Enteritidis Infection in Mice Is Associated with Th Polarization. Infect. Immun., May 1, 2006; 74(5): 2787 - 2802. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S.-i. Hiraoka, N. Takeuchi, Y. Bian, H. Nakahara, M. Kogo, K. Dunussi-Joannopoulos, S. Wolf, S. Ono, and H. Fujiwara B7.2-Ig fusion proteins enhance IL-4-dependent differentiation of tumor-sensitized CD8+ cytotoxic T lymphocyte precursors Int. Immunol., August 1, 2005; 17(8): 1071 - 1079. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Bian, S.-i. Hiraoka, M. Tomura, X.-Y. Zhou, Y. Yashiro-Ohtani, Y. Mori, J. Shimizu, S. Ono, K. Dunussi-Joannopoulos, S. Wolf, et al. The capacity of the natural ligands for CD28 to drive IL-4 expression in naive and antigen-primed CD4+ and CD8+ T cells Int. Immunol., January 1, 2005; 17(1): 73 - 83. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Zhang, M. Martin, Q.-B. Yang, S. M. Michalek, and J. Katz Role of B7 Costimulatory Molecules in Immune Responses and T-Helper Cell Differentiation in Response to Recombinant HagB from Porphyromonas gingivalis Infect. Immun., February 1, 2004; 72(2): 637 - 644. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Yamaguchi, S.-i. Hiraoka, T. Mukai, N. Takeuchi, X.-Y. Zhou, S. Ono, M. Kogo, K. Dunussi-Joannopoulos, V. Ling, S. Wolf, et al. Induction of Tumor Regression by Administration of B7-Ig Fusion Proteins: Mediation by Type 2 CD8+ T Cells and Dependence on IL-4 Production J. Immunol., February 1, 2004; 172(3): 1347 - 1354. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Vanhove, G. Laflamme, F. Coulon, M. Mougin, P. Vusio, F. Haspot, J. Tiollier, and J.-P. Soulillou Selective blockade of CD28 and not CTLA-4 with a single-chain Fv-{alpha}1-antitrypsin fusion antibody Blood, July 15, 2003; 102(2): 564 - 570. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. V. Parry, C. A. Rumbley, L. H. Vandenberghe, C. H. June, and J. L. Riley CD28 and Inducible Costimulatory Protein Src Homology 2 Binding Domains Show Distinct Regulation of Phosphatidylinositol 3-Kinase, Bcl-xL, and IL-2 Expression in Primary Human CD4 T Lymphocytes J. Immunol., July 1, 2003; 171(1): 166 - 174. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Mukherjee, P. K. Maiti, and D. Nandi Role of CD80, CD86, and CTLA4 on mouse CD4+ T lymphocytes in enhancing cell-cycle progression and survival after activation with PMA and ionomycin J. Leukoc. Biol., November 1, 2002; 72(5): 921 - 931. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Q.-B. Yang, M. Martin, S. M. Michalek, and J. Katz Mechanisms of Monophosphoryl Lipid A Augmentation of Host Responses to Recombinant HagB from Porphyromonas gingivalis Infect. Immun., July 1, 2002; 70(7): 3557 - 3565. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. L. Compton and J. P. Farrell CD28 Costimulation and Parasite Dose Combine to Influence the Susceptibility of BALB/c Mice to Infection with Leishmania major J. Immunol., February 1, 2002; 168(3): 1302 - 1308. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Saverino, A. Merlo, S. Bruno, V. Pistoia, C. E. Grossi, and E. Ciccone Dual Effect of CD85/Leukocyte Ig-Like Receptor-1/Ig-Like Transcript 2 and CD152 (CTLA-4) on Cytokine Production by Antigen-Stimulated Human T Cells J. Immunol., January 1, 2002; 168(1): 207 - 215. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
