| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Departments of
*
Pathology and
Microbiology and Immunology, Uniformed Services University of the Health Sciences, Bethesda, MD 20814;
Biosynexus, Rockville, MD 20850; and
§
Department of Immunology and Inflammation, Bristol-Myers Squibb, Princeton, NJ 08543
The requirements for B7 costimulation during an in vivo humoral response to an intact extracellular bacteria have not been reported. In this study we immunized mice with Streptococcus pneumoniae (R36A) to determine the B7 requirements for induction of Ig, specific for two determinants on R36A, the phosphorylcholine (PC) determinant of C-polysaccharide and pneumococcal surface protein A (PspA). We show that the primary anti-PspA response, the development of PspA-specific memory, and the induction of the secondary anti-PspA response in primed mice were completely dependent upon B7 costimulation. Of note, costimulation was required only briefly after the secondary immunization compared with after the primary immunization for optimal induction of Ig. Blockade of B7 costimulation at the time of secondary immunization also completely abrogated the established state of memory, but did not induce tolerance. In contrast to the anti-PspA response, the primary anti-PC response involved only a very short period of B7 costimulation. Whereas B7-2 alone was required for induction of the primary anti-PspA and anti-PC responses, a redundant role for B7-1 and B7-2 was noted for the PspA-specific secondary response. CTLA4Ig blocked both the anti-PC and anti-PspA responses equally well over a wide range of bacterial doses. These studies demonstrate a critical, but variable, role for B7-dependent costimulation during an Ig response to an extracellular bacteria.
This article has been cited by other articles:
|
|
 |
|
  G. Chattopadhyay, Q. Chen, J. Colino, A. Lees, and C. M. Snapper Intact Bacteria Inhibit the Induction of Humoral Immune Responses to Bacterial-Derived and Heterologous Soluble T Cell-Dependent Antigens J. Immunol., February 15, 2009; 182(4): 2011 - 2019. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Q. Chen, J. L. Cannons, J. C. Paton, H. Akiba, P. L. Schwartzberg, and C. M. Snapper A Novel ICOS-Independent, but CD28- and SAP-Dependent, Pathway of T Cell-Dependent, Polysaccharide-Specific Humoral Immunity in Response to Intact Streptococcus pneumoniae versus Pneumococcal Conjugate Vaccine J. Immunol., December 15, 2008; 181(12): 8258 - 8266. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Moens, A. Jeurissen, G. Wuyts, P. G. Fallon, B. Louis, J. L. Ceuppens, and X. Bossuyt Specific Intracellular Adhesion Molecule-Grabbing Nonintegrin R1 Is Not Involved in the Murine Antibody Response to Pneumococcal Polysaccharides Infect. Immun., December 1, 2007; 75(12): 5748 - 5752. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Chattopadhyay, A. Q. Khan, G. Sen, J. Colino, W. duBois, A. Rubtsov, R. M. Torres, M. Potter, and C. M. Snapper Transgenic Expression of Bcl-xL or Bcl-2 by Murine B Cells Enhances the In Vivo Antipolysaccharide, but Not Antiprotein, Response to Intact Streptococcus pneumoniae J. Immunol., December 1, 2007; 179(11): 7523 - 7534. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. G. Thebeau, S. P. Vagvala, Y. M. Wong, and L. A. Morrison B7 Costimulation Molecules Expressed from the Herpes Simplex Virus 2 Genome Rescue Immune Induction in B7-Deficient Mice J. Virol., November 15, 2007; 81(22): 12200 - 12209. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Kaur, S. Chowdhury, N. S. Greenspan, and J. R. Schreiber Decreased expression of tumor necrosis factor family receptors involved in humoral immune responses in preterm neonates Blood, October 15, 2007; 110(8): 2948 - 2954. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Q. Chen, G. Sen, and C. M. Snapper Endogenous IL-1R1 Signaling Is Critical for Cognate CD4+ T Cell Help for Induction of In Vivo Type 1 and Type 2 Antipolysaccharide and Antiprotein Ig Isotype Responses to Intact Streptococcus pneumoniae, but Not to a Soluble Pneumococcal Conjugate Vaccine J. Immunol., November 1, 2006; 177(9): 6044 - 6051. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. D. Salazar, M. R. Miller, J. B. Barnett, and R. Schafer Evidence for a Novel Endocrine Disruptor: The Pesticide Propanil Requires the Ovaries and Steroid Synthesis to Enhance Humoral Immunity Toxicol. Sci., September 1, 2006; 93(1): 62 - 74. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Sen, Q. Chen, and C. M. Snapper Immunization of Aged Mice with a Pneumococcal Conjugate Vaccine Combined with an Unmethylated CpG-Containing Oligodeoxynucleotide Restores Defective Immunoglobulin G Antipolysaccharide Responses and Specific CD4+-T-Cell Priming to Young Adult Levels Infect. Immun., April 1, 2006; 74(4): 2177 - 2186. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Boudewijns, A. Jeurissen, M. Wuyts, L. Moens, L. Boon, J. J. Van Neerven, A. Kasran, L. Overbergh, C. Lenaerts, M. Waer, et al. Blockade of CTLA-4 (CD152) enhances the murine antibody response to pneumococcal capsular polysaccharides J. Leukoc. Biol., November 1, 2005; 78(5): 1060 - 1069. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. D. Salazar, P. de la Rosa, J. B. Barnett, and R. Schafer The Polysaccharide Antibody Response after Streptococcus pneumoniae Vaccination Is Differentially Enhanced or Suppressed by 3,4-Dichloropropionanilide and 2,4-Dichlorophenoxyacetic Acid Toxicol. Sci., September 1, 2005; 87(1): 123 - 133. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Q. Khan, Q. Chen, Z.-Q. Wu, J. C. Paton, and C. M. Snapper Both Innate Immunity and Type 1 Humoral Immunity to Streptococcus pneumoniae Are Mediated by MyD88 but Differ in Their Relative Levels of Dependence on Toll-Like Receptor 2 Infect. Immun., January 1, 2005; 73(1): 298 - 307. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Y. Balkhi, V. K. Latchumanan, B. Singh, P. Sharma, and K. Natarajan Cross-regulation of CD86 by CD80 differentially regulates T helper responses from Mycobacterium tuberculosis secretory antigen-activated dendritic cell subsets J. Leukoc. Biol., May 1, 2004; 75(5): 874 - 883. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Zhang, M. Martin, Q.-B. Yang, S. M. Michalek, and J. Katz Role of B7 Costimulatory Molecules in Immune Responses and T-Helper Cell Differentiation in Response to Recombinant HagB from Porphyromonas gingivalis Infect. Immun., February 1, 2004; 72(2): 637 - 644. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Q. Khan, A. Lees, and C. M. Snapper Differential Regulation of IgG Anti-Capsular Polysaccharide and Antiprotein Responses to Intact Streptococcus pneumoniae in the Presence of Cognate CD4+ T Cell Help J. Immunol., January 1, 2004; 172(1): 532 - 539. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Colino and C. M. Snapper Opposing Signals from Pathogen-Associated Molecular Patterns and IL-10 Are Critical for Optimal Dendritic Cell Induction of In Vivo Humoral Immunity to Streptococcus pneumoniae J. Immunol., October 1, 2003; 171(7): 3508 - 3519. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z.-Q. Wu, A. Q. Khan, Y. Shen, K. M. Wolcott, W. Dawicki, T. H. Watts, R. S. Mittler, and C. M. Snapper 4-1BB (CD137) Differentially Regulates Murine In Vivo Protein- and Polysaccharide-Specific Immunoglobulin Isotype Responses to Streptococcus pneumoniae Infect. Immun., January 1, 2003; 71(1): 196 - 204. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z.-Q. Wu, Y. Shen, A. Q. Khan, C.-L. Chu, R. Riese, H. A. Chapman, O. Kanagawa, and C. M. Snapper The Mechanism Underlying T Cell Help for Induction of an Antigen-Specific In Vivo Humoral Immune Response to Intact Streptococcus pneumoniae Is Dependent on the Type of Antigen J. Immunol., June 1, 2002; 168(11): 5551 - 5557. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Q. Khan, Y. Shen, Z.-Q. Wu, T. A. Wynn, and C. M. Snapper Endogenous Pro- and Anti-Inflammatory Cytokines Differentially Regulate an In Vivo Humoral Response to Streptococcus pneumoniae Infect. Immun., February 1, 2002; 70(2): 749 - 761. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Colino, Y. Shen, and C. M. Snapper Dendritic Cells Pulsed with Intact Streptococcus pneumoniae Elicit both Protein- and Polysaccharide-specific Immunoglobulin Isotype Responses In Vivo through Distinct Mechanisms J. Exp. Med., December 31, 2001; 195(1): 1 - 14. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
