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Beirne B. Carter Center for Immunology Research and
Departments of Microbiology and Pathology, University of Virginia Health Sciences Center, Charlottesville, VA 22908
BALB/c mice immunized with a vaccinia virus expressing the
attachment (G) glycoprotein of respiratory syncytial virus (RSV)
develop a virus-specific CD4+ T cell response that consists
of a mixture of Th1 and Th2 CD4+ T cells following
intranasal infection with live RSV. Recent work has shown that both Th1
and Th2 CD4+ T cells are elicited to a single region
comprising aa 183–197 of the G protein. To more precisely define the
CD4+ T cell epitope(s) contained within this region, we
created a panel of amino- and carboxyl-terminal truncated as well as
single alanine-substituted peptides spanning aa 183–197. These
peptides were used to examine the ex vivo cytokine response of memory
effector CD4+ T cells infiltrating the lungs of G-primed
RSV-infected mice. Analysis of lung-derived memory effector
CD4+ T cells using intracellular cytokine staining and/or
ELISA of effector T cell culture supernatants revealed a single
I-Ed-restricted CD4+ T cell epitope with a core
sequence mapping to aa 185–193. In addition, we examined the T cell
repertoire of the RSV G peptide-specific CD4+ T cells and
show that the CD4+ T cells directed to this single
immunodominant G epitope use a restricted range of TCR V
genes and
predominantly express V14 TCR.
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