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Gene Structure and Promoter Variation of Expressed and Nonexpressed Variants of the KIR2DL5 Gene^{1 ,2}

Departments of Structural Biology and Microbiology and Immunology, Stanford University School of Medicine, Stanford, CA 94305

Two variants of the novel KIR2DL5 gene (KIR2DL5.1 and .2) were identified in genomic DNA of a single donor. However, only the KIR2DL5.1 variant was transcribed in PBMC. In this study, analysis of seven additional donors reveals two new variants of the KIR2DL5 gene and indicates that transcription, or its lack, are consistently associated with particular variants of this gene. Comparison of the complete nucleotide sequences of the exons and introns of KIR2DL5.1 and KIR2DL5.2 reveals no structural abnormalities, but similar open reading frames for both variants. In contrast, the promoter region of KIR2DL5 shows a high degree of sequence polymorphism that is likely relevant for expression. Substitution within a putative binding site for the transcription factor acute myeloid leukemia gene 1 could determine the lack of expression for some KIR2DL5 variants.

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