HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Volgina, V. V. Articles by Knight, K. L. Search for Related Content PubMed PubMed Citation Articles by Volgina, V. V. Articles by Knight, K. L.

A Single 3' hs1,2 Enhancer in the Rabbit IgH Locus¹

Department of Microbiology and Immunology, Loyola University Chicago, Maywood, IL 60153

Multiple cis-acting elements including the intronic enhancer and the 3' enhancer (3'E) regulate expression of the Ig heavy chain genes during B cell development. A 3'E is composed of DNase I-hypersensitive sites, hs1,2, hs3a,b, and hs4, found 3' of the murine C gene as well as 3' of both human C genes, C1 and C2. Rabbits have 13 C genes, and we tested whether a 3'E is associated with each of these genes. To identify 3'E regions we developed a rabbit hs1,2 probe and used this to search for enhancer homologues of human hs1,2 in a genomic fosmid library. We identified a single hs1,2 fragment 8-kb downstream of C13, the presumed 3'-most C gene. We also identified and partially sequenced a new C gene, C14, located 6 kb upstream of C13. Genomic Southern blot analysis confirmed that the rabbit genome contains only one hs1,2 enhancer region. We tested the enhancer activity of the hs1,2 with the SV40, V_H, and I promoters using the luciferase reporter gene in transient transfection assays and found that it significantly enhanced the activity of SV40 and V_H promoters and slightly enhanced an I promoter. We conclude that the rabbit has a single hs1,2 enhancer that resides at the 3' end of the IgH gene cluster and may constitute one of the cis-elements regulating the expression of IgH genes.

This article has been cited by other articles:

				V. Volgina, P.-C. Yam, and K. L. Knight A negative regulatory element in the rabbit 3'IgH chromosomal region Int. Immunol., August 1, 2005; 17(8): 973 - 982. [Abstract] [Full Text] [PDF]

				H. Spieker-Polet, P.-C. Yam, and K. L. Knight Functional Analysis of I{alpha} Promoter Regions of Multiple IgA Heavy Chain Genes J. Immunol., April 1, 2002; 168(7): 3360 - 3368. [Abstract] [Full Text] [PDF]