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The Journal of Immunology, 2000, 165: 5435-5442.
Copyright © 2000 by The American Association of Immunologists

Immature CD4+CD8+ Thymocytes Do Not Polarize Lipid Rafts in Response to TCR-Mediated Signals1

Peter J. R. Ebert, Josh F. Baker and Jennifer A. Punt2

Department of Biology, Haverford College, Haverford, PA 19041

TCR-mediated stimulation induces activation and proliferation of mature T cells. When accompanied by signals through the costimulatory receptor CD28, TCR signals also result in the recruitment of cholesterol- and glycosphingolipid-rich membrane microdomains (lipid rafts), which are known to contain several molecules important for T cell signaling. Interestingly, immature CD4+CD8+ thymocytes respond to TCR/CD28 costimulation not by proliferating, but by dying. In this study, we report that, although CD4+CD8+ thymocytes polarize their actin cytoskeleton, they fail to recruit lipid rafts to the site of TCR/CD28 costimulation. We show that coupling of lipid raft mobilization to cytoskeletal reorganization can be mediated by phosphoinositide 3-kinase, and discuss the relevance of these findings to the interpretation of TCR signals by immature vs mature T cells.




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