| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Departments of
*
Immunobiology,
Research Administration, and
Analytical Chemistry and Formulation, Immunex Corporation, Seattle, WA 98101; and
§
The Walter and Eliza Hall Institute of Medical Research, Melbourne, Australia
Dendritic cells (DC) are potent APCs that can be characterized in
the murine spleen as CD11bhighCD11chigh or
CD11blowCD11chigh. Daily injection of mice of
Flt3 ligand (FL) into mice transiently expands both subsets of DC in
vivo, but the effect of administration of GM-CSF on the expansion of DC
in vivo is not well defined. To gain further insight into the role of
GM-CSF in DC development and function in vivo, we treated mice with
polyethylene glycol-modified GM-CSF (pGM-CSF) which has an increased
half-life in vivo. Administration of pGM-CSF to mice for 5 days led to
a 5- to 10-fold expansion of CD11bhighCD11chigh
but not CD11blowCD11chigh DC. DC from
pGM-CSF-treated mice captured and processed Ag more efficiently than DC
from FL-treated mice. Although both FL- and pGM-CSF-generated
CD11bhighCD11chigh DC were
CD8
-, a greater proportion of these DC from
pGM-CSF-treated mice were 33D1+ than from FL-treated mice.
CD11blowCD11chigh DC from FL-treated mice
expressed high levels of intracellular MHC class II. DC from both
pGM-CSF- and FL-treated mice expressed high levels of surface class II,
low levels of the costimulatory molecules CD40, CD80, and CD86 and were
equally efficient at stimulating allogeneic and Ag-specific T cell
proliferation in vitro. The data demonstrate that treatment with
pGM-CSF in vivo preferentially expands
CD11bhighCD11chigh DC that share phenotypic and
functional characteristics with FL-generated
CD11bhighCD11chigh DC but can be distinguished
from FL-generated DC on the basis of Ag capture and surface expression
of 33D1.
This article has been cited by other articles:
|
|
 |
|
  Z. Shao, T. O. Makinde, H. S. McGee, X. Wang, and D. K. Agrawal Fms-Like Tyrosine Kinase 3 Ligand Regulates Migratory Pattern and Antigen Uptake of Lung Dendritic Cell Subsets in a Murine Model of Allergic Airway Inflammation J. Immunol., December 1, 2009; 183(11): 7531 - 7538. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 O. A. Ali, D. Emerich, G. Dranoff, and D. J. Mooney In Situ Regulation of DC Subsets and T Cells Mediates Tumor Regression in Mice Science Translational Medicine, November 25, 2009; 1(8): 8ra19 - 8ra19. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S.-N. Zhu, M. Chen, J. Jongstra-Bilen, and M. I. Cybulsky GM-CSF regulates intimal cell proliferation in nascent atherosclerotic lesions J. Exp. Med., September 28, 2009; 206(10): 2141 - 2149. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Zarei, F. Schwenter, P. Luy, M. Aurrand-Lions, P. Morel, M. Kopf, G. Dranoff, and N. Mach Role of GM-CSF signaling in cell-based tumor immunization Blood, June 25, 2009; 113(26): 6658 - 6668. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Ramakrishna, N. Woller, B. Mundt, S. Knocke, E. Gurlevik, M. Saborowski, N. Malek, M. P. Manns, T. Wirth, F. Kuhnel, et al. Antitumoral Immune Response by Recruitment and Expansion of Dendritic Cells in Tumors Infected with Telomerase-Dependent Oncolytic Viruses Cancer Res., February 15, 2009; 69(4): 1448 - 1458. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. R. Mott and H. Ghiasi Role of Dendritic Cells in Enhancement of Herpes Simplex Virus Type 1 Latency and Reactivation in Vaccinated Mice Clin. Vaccine Immunol., December 1, 2008; 15(12): 1859 - 1867. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. R. Mott, D. UnderHill, S. L. Wechsler, and H. Ghiasi Lymphoid-Related CD11c+ CD8{alpha}+ Dendritic Cells Are Involved in Enhancing Herpes Simplex Virus Type 1 Latency J. Virol., October 15, 2008; 82(20): 9870 - 9879. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Carreras, S. Turner, V. Paharkova-Vatchkova, A. Mao, C. Dascher, and S. Kovats Estradiol Acts Directly on Bone Marrow Myeloid Progenitors to Differentially Regulate GM-CSF or Flt3 Ligand-Mediated Dendritic Cell Differentiation J. Immunol., January 15, 2008; 180(2): 727 - 738. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. A. Ryan, T. M. Wozniak, E. Shklovskaya, M. A. O'Donnell, B. Fazekas de St. Groth, W. J. Britton, and J. A. Triccas Improved Protection against Disseminated Tuberculosis by Mycobacterium bovis Bacillus Calmette-Guerin Secreting Murine GM-CSF Is Associated with Expansion and Activation of APCs J. Immunol., December 15, 2007; 179(12): 8418 - 8424. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Winter, K. Taut, F. Langer, M. Mack, D. E. Briles, J. C. Paton, R. Maus, M. Srivastava, T. Welte, and U. A. Maus FMS-Like Tyrosine Kinase 3 Ligand Aggravates the Lung Inflammatory Response to Streptococcus pneumoniae Infection in Mice: Role of Dendritic Cells J. Immunol., September 1, 2007; 179(5): 3099 - 3108. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. S. Bandukwala, B. S. Clay, J. Tong, P. D. Mody, J. L. Cannon, R. A. Shilling, J. S. Verbeek, J. V. Weinstock, J. Solway, and A. I. Sperling Signaling through Fc{gamma}RIII is required for optimal T helper type (Th)2 responses and Th2-mediated airway inflammation J. Exp. Med., August 6, 2007; 204(8): 1875 - 1889. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. R. Sheng, L. Li, B. B. Ganesh, C. Vasu, B. S. Prabhakar, and M. N. Meriggioli Suppression of Experimental Autoimmune Myasthenia Gravis by Granulocyte-Macrophage Colony-Stimulating Factor Is Associated with an Expansion of FoxP3+ Regulatory T Cells J. Immunol., October 15, 2006; 177(8): 5296 - 5306. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Chavan, K. A. Marfatia, I. C. An, D. A. Garber, and M. B. Feinberg Expression of CCL20 and Granulocyte-Macrophage Colony-Stimulating Factor, but Not Flt3-L, from Modified Vaccinia Virus Ankara Enhances Antiviral Cellular and Humoral Immune Responses. J. Virol., August 1, 2006; 80(15): 7676 - 7687. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C.W. Cutler and R. Jotwani Dendritic Cells at the Oral Mucosal Interface Journal of Dental Research, August 1, 2006; 85(8): 678 - 689. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Berhanu, J. Huang, S. M. Alber, S. C. Watkins, and W. J. Storkus Combinational FLt3 Ligand and Granulocyte Macrophage Colony-Stimulating Factor Treatment Promotes Enhanced Tumor Infiltration by Dendritic Cells and Antitumor CD8+ T-Cell Cross-priming but Is Ineffective as a Therapy. Cancer Res., May 1, 2006; 66(9): 4895 - 4903. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. I. Slukvin, M. A. Vodyanik, J. A. Thomson, M. E. Gumenyuk, and K.-D. Choi Directed Differentiation of Human Embryonic Stem Cells into Functional Dendritic Cells through the Myeloid Pathway. J. Immunol., March 1, 2006; 176(5): 2924 - 2932. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Wahid, M. J. Cannon, and M. Chow Dendritic Cells and Macrophages Are Productively Infected by Poliovirus J. Virol., January 1, 2005; 79(1): 401 - 409. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Driessens, M. Hamdane, V. Cool, T. Velu, and C. Bruyns Highly Successful Therapeutic Vaccinations Combining Dendritic Cells and Tumor Cells Secreting Granulocyte Macrophage Colony-stimulating Factor Cancer Res., November 15, 2004; 64(22): 8435 - 8442. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. N. Hudson Keenihan and S. A. Robertson Diversity in Phenotype and Steroid Hormone Dependence in Dendritic Cellsand Macrophages in the Mouse Uterus Biol Reprod, June 1, 2004; 70(6): 1562 - 1572. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Peng, S. F. Hussain, and Y. Paterson The Ability of Two Listeria monocytogenes Vaccines Targeting Human Papillomavirus-16 E7 to Induce an Antitumor Response Correlates with Myeloid Dendritic Cell Function J. Immunol., May 15, 2004; 172(10): 6030 - 6038. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Chklovskaia, P. Nowbakht, C. Nissen, A. Gratwohl, M. Bargetzi, and A. Wodnar-Filipowicz Reconstitution of dendritic and natural killer-cell subsets after allogeneic stem cell transplantation: effects of endogenous flt3 ligand Blood, May 15, 2004; 103(10): 3860 - 3868. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. C. Alaniz, S. Sandall, E. K. Thomas, and C. B. Wilson Increased Dendritic Cell Numbers Impair Protective Immunity to Intracellular Bacteria Despite Augmenting Antigen-Specific CD8+ T Lymphocyte Responses J. Immunol., March 15, 2004; 172(6): 3725 - 3735. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Vasu, R.-N. E. Dogan, M. J. Holterman, and B. S. Prabhakar Selective Induction of Dendritic Cells Using Granulocyte Macrophage-Colony Stimulating Factor, But Not fms-Like Tyrosine Kinase Receptor 3-Ligand, Activates Thyroglobulin-Specific CD4+/CD25+ T Cells and Suppresses Experimental Autoimmune Thyroiditis J. Immunol., June 1, 2003; 170(11): 5511 - 5522. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Granucci, S. Feau, V. Angeli, F. Trottein, and P. Ricciardi-Castagnoli Early IL-2 Production by Mouse Dendritic Cells Is the Result of Microbial-Induced Priming J. Immunol., May 15, 2003; 170(10): 5075 - 5081. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Guebre-Xabier, S. A. Hammond, D. E. Epperson, J. Yu, L. Ellingsworth, and G. M. Glenn Immunostimulant Patch Containing Heat-Labile Enterotoxin from Escherichia coli Enhances Immune Responses to Injected Influenza Virus Vaccine through Activation of Skin Dendritic Cells J. Virol., May 1, 2003; 77(9): 5218 - 5225. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Miller, V. G. Pillarisetty, A. B. Shah, S. Lahrs, and R. P. DeMatteo Murine Flt3 Ligand Expands Distinct Dendritic Cells with Both Tolerogenic and Immunogenic Properties J. Immunol., April 1, 2003; 170(7): 3554 - 3564. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R.-N. E. Dogan, C. Vasu, M. J. Holterman, and B. S. Prabhakar Absence of IL-4, and Not Suppression of the Th2 Response, Prevents Development of Experimental Autoimmune Graves' Disease J. Immunol., February 15, 2003; 170(4): 2195 - 2204. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Miller, V. G. Pillarisetty, A. B. Shah, S. Lahrs, Z. Xing, and R. P. DeMatteo Endogenous Granulocyte-Macrophage Colony-Stimulating Factor Overexpression In Vivo Results in the Long-Term Recruitment of a Distinct Dendritic Cell Population with Enhanced Immunostimulatory Function J. Immunol., September 15, 2002; 169(6): 2875 - 2885. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
U. Yrlid and M. J. Wick Antigen Presentation Capacity and Cytokine Production by Murine Splenic Dendritic Cell Subsets upon Salmonella Encounter J. Immunol., July 1, 2002; 169(1): 108 - 116. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. K. Basak, A. Harui, M. Stolina, S. Sharma, K. Mitani, S. M. Dubinett, and M. D. Roth Increased dendritic cell number and function following continuous in vivo infusion of granulocyte macrophage-colony-stimulating factor and interleukin-4 Blood, April 15, 2002; 99(8): 2869 - 2879. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. O'Keeffe, H. Hochrein, D. Vremec, J. Pooley, R. Evans, S. Woulfe, and K. Shortman Effects of administration of progenipoietin 1, Flt-3 ligand, granulocyte colony-stimulating factor, and pegylated granulocyte-macrophage colony-stimulating factor on dendritic cell subsets in mice Blood, March 15, 2002; 99(6): 2122 - 2130. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. F. Lipscomb and B. J. Masten Dendritic Cells: Immune Regulators in Health and Disease Physiol Rev, January 1, 2002; 82(1): 97 - 130. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. W. Cutler, R. Jotwani, and B. Pulendran Dendritic Cells: Immune Saviors or Achilles' Heel? Infect. Immun., August 1, 2001; 69(8): 4703 - 4708. [Full Text] [PDF]
|
|
|
|
|
|
S. Henri, D. Vremec, A. Kamath, J. Waithman, S. Williams, C. Benoist, K. Burnham, S. Saeland, E. Handman, and K. Shortman The Dendritic Cell Populations of Mouse Lymph Nodes J. Immunol., July 15, 2001; 167(2): 741 - 748. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G.B. Toews Cytokines and the lung Eur. Respir. J., July 2, 2001; 18(34_suppl): 3S - 17s. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. C. Kirby, U. Yrlid, M. Svensson, and M. J. Wick Differential Involvement of Dendritic Cell Subsets During Acute Salmonella Infection J. Immunol., June 1, 2001; 166(11): 6802 - 6811. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Bronte, E. Apolloni, A. Cabrelle, R. Ronca, P. Serafini, P. Zamboni, N. P. Restifo, and P. Zanovello Identification of a CD11b+/Gr-1+/CD31+ myeloid progenitor capable of activating or suppressing CD8+ T cells Blood, December 1, 2000; 96(12): 3838 - 3846. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
