| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
*
Department of Immunology, The Weizmann Institute of Science, Rehovot, Israel; and
Department of Tumor Immunology, University Hospital Nijmegen, Nijmegen, The Netherlands
The LFA-1 integrin is crucial for the firm adhesion of circulating leukocytes to ICAM-1-expressing endothelial cells. In the present study, we demonstrate that LFA-1 can arrest unstimulated PBL subsets and lymphoblastoid Jurkat cells on immobilized ICAM-1 under subphysiological shear flow and mediate firm adhesion to ICAM-1 after short static contact. However, LFA-1 expressed in K562 cells failed to support firm adhesion to ICAM-1 but instead mediated K562 cell rolling on the endothelial ligand under physiological shear stress. LFA-1-mediated rolling required an intact LFA-1 I-domain, was enhanced by Mg2+, and was sharply dependent on ICAM-1 density. This is the first indication that LFA-1 can engage in rolling adhesions with ICAM-1 under physiological shear flow. The ability of LFA-1 to support rolling correlates with decreased avidity and impaired time-dependent adhesion strengthening. A ß2 cytoplasmic domain-deletion mutant of LFA-1, with high avidity to immobilized ICAM-1, mediated firm arrests of K562 cells interacting with ICAM-1 under shear flow. Our results suggest that restrictions in LFA-1 clustering mediated by cytoskeletal attachments may lock the integrin into low-avidity states in particular cellular environments. Although low-avidity LFA-1 states fail to undergo adhesion strengthening upon contact with ICAM-1 at stasis, these states are permissive for leukocyte rolling on ICAM-1 under physiological shear flow. Rolling mediated by low-avidity LFA-1 interactions with ICAM-1 may stabilize rolling initiated by specialized vascular rolling receptors and allow the leukocyte to arrest on vascular endothelium upon exposure to stimulatory endothelial signals.
This article has been cited by other articles:
|
|
 |
|
  L. Flaishon, G. Hart, E. Zelman, C. Moussion, V. Grabovsky, G. Lapidot Tal, S. Feigelson, R. Margalit, A. Harmelin, T. Avin-Wittenberg, et al. Anti-inflammatory effects of an inflammatory chemokine: CCL2 inhibits lymphocyte homing by modulation of CCL21-triggered integrin-mediated adhesions Blood, December 15, 2008; 112(13): 5016 - 5025. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. N. Hartmann, V. Grabovsky, R. Pasvolsky, Z. Shulman, E. C. Buss, A. Spiegel, A. Nagler, T. Lapidot, M. Thelen, and R. Alon A crosstalk between intracellular CXCR7 and CXCR4 involved in rapid CXCL12-triggered integrin activation but not in chemokine-triggered motility of human T lymphocytes and CD34+ cells J. Leukoc. Biol., October 1, 2008; 84(4): 1130 - 1140. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X.-Y. Tang, Y.-F. Li, and S.-M. Tan Intercellular Adhesion Molecule-3 Binding of Integrin {alpha}L{beta}2 Requires Both Extension and Opening of the Integrin Headpiece J. Immunol., April 1, 2008; 180(7): 4793 - 4804. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Pasvolsky, V. Grabovsky, C. Giagulli, Z. Shulman, R. Shamri, S. W. Feigelson, C. Laudanna, and R. Alon RhoA Is Involved in LFA-1 Extension Triggered by CXCL12 but Not in a Novel Outside-In LFA-1 Activation Facilitated by CXCL9 J. Immunol., March 1, 2008; 180(5): 2815 - 2823. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Manevich, V. Grabovsky, S. W. Feigelson, and R. Alon Talin 1 and Paxillin Facilitate Distinct Steps in Rapid VLA-4-mediated Adhesion Strengthening to Vascular Cell Adhesion Molecule 1 J. Biol. Chem., August 31, 2007; 282(35): 25338 - 25348. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Salas, M. Shimaoka, U. Phan, M. Kim, and T. A. Springer Transition from Rolling to Firm Adhesion Can Be Mimicked by Extension of Integrin {alpha}Lbeta2 in an Intermediate Affinity State J. Biol. Chem., April 21, 2006; 281(16): 10876 - 10882. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. W. Stief, A. Richter, R. Bunder, B. Maisch, and H. Renz Monitoring of Plasmin and Plasminogen Activator Activity in Blood of Patients under Fibrinolytic Treatment by Reteplase Clinical and Applied Thrombosis/Hemostasis, April 1, 2006; 12(2): 213 - 218. [Abstract] [PDF]
|
|
|
|
|
|
C. E. Green, U. Y. Schaff, M. R. Sarantos, A. F. H. Lum, D. E. Staunton, and S. I. Simon Dynamic shifts in LFA-1 affinity regulate neutrophil rolling, arrest, and transmigration on inflamed endothelium Blood, March 1, 2006; 107(5): 2101 - 2111. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Alon, S. W. Feigelson, E. Manevich, D. M. Rose, J. Schmitz, D. R. Overby, E. Winter, V. Grabovsky, V. Shinder, B. D. Matthews, et al. {alpha}4{beta}1-dependent adhesion strengthening under mechanical strain is regulated by paxillin association with the {alpha}4-cytoplasmic domain J. Cell Biol., December 19, 2005; 171(6): 1073 - 1084. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. R. Sarantos, S. Raychaudhuri, A. F. H. Lum, D. E. Staunton, and S. I. Simon Leukocyte Function-associated Antigen 1-mediated Adhesion Stability Is Dynamically Regulated through Affinity and Valency during Bond Formation with Intercellular Adhesion Molecule-1 J. Biol. Chem., August 5, 2005; 280(31): 28290 - 28298. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. A. Rosenthal-Allieri, M. Ticchioni, J. P. Breittmayer, Y. Shimizu, and A. Bernard Influence of {beta}1 Integrin Intracytoplasmic Domains in the Regulation of VLA-4-Mediated Adhesion of Human T Cells to VCAM-1 under Flow Conditions J. Immunol., July 15, 2005; 175(2): 1214 - 1223. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Huang, K. Matthews, T. J. Siahaan, and C. G. Kevil {alpha}L-Integrin I domain cyclic peptide antagonist selectively inhibits T cell adhesion to pancreatic islet microvascular endothelium Am J Physiol Gastrointest Liver Physiol, January 1, 2005; 288(1): G67 - G73. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. P. Burns and N. DePaola Flow-conditioned HUVECs support clustered leukocyte adhesion by coexpressing ICAM-1 and E-selectin Am J Physiol Heart Circ Physiol, January 1, 2005; 288(1): H194 - H204. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. W. Feigelson, V. Grabovsky, R. Shamri, S. Levy, and R. Alon The CD81 Tetraspanin Facilitates Instantaneous Leukocyte VLA-4 Adhesion Strengthening to Vascular Cell Adhesion Molecule 1 (VCAM-1) under Shear Flow J. Biol. Chem., December 19, 2003; 278(51): 51203 - 51212. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. van Wetering, N. van den Berk, J. D. van Buul, F. P. J. Mul, I. Lommerse, R. Mous, J.-P. t. Klooster, J.-J. Zwaginga, and P. L. Hordijk VCAM-1-mediated Rac signaling controls endothelial cell-cell contacts and leukocyte transmigration Am J Physiol Cell Physiol, August 1, 2003; 285(2): C343 - C352. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. G. Kevil, J. H. Chidlow, D. C. Bullard, and D. F. Kucik High-temporal-resolution analysis demonstrates that ICAM-1 stabilizes WEHI 274.1 monocytic cell rolling on endothelium Am J Physiol Cell Physiol, July 1, 2003; 285(1): C112 - C118. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Salas, M. Shimaoka, S. Chen, C. V. Carman, and T. Springer Transition From Rolling to Firm Adhesion Is Regulated by the Conformation of the I Domain of the Integrin Lymphocyte Function-associated Antigen-1 J. Biol. Chem., December 20, 2002; 277(52): 50255 - 50262. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Kadono, G. M. Venturi, D. A. Steeber, and T. F. Tedder Leukocyte Rolling Velocities and Migration Are Optimized by Cooperative L-Selectin and Intercellular Adhesion Molecule-1 Functions J. Immunol., October 15, 2002; 169(8): 4542 - 4550. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Shamri, V. Grabovsky, S. W. Feigelson, O. Dwir, Y. van Kooyk, and R. Alon Chemokine Stimulation of Lymphocyte alpha 4 Integrin Avidity but Not of Leukocyte Function-associated Antigen-1 Avidity to Endothelial Ligands under Shear Flow Requires Cholesterol Membrane Rafts J. Biol. Chem., October 11, 2002; 277(42): 40027 - 40035. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. W. de Rossi, N. A. Horn, W. Buhre, F. Gass, G. Hutschenreuter, and R. Rossaint The Effect of Isoflurane on Neutrophil Selectin and {beta}2-Integrin Activation In Vitro Anesth. Analg., September 1, 2002; 95(3): 583 - 587. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. F. H. Lum, C. E. Green, G. R. Lee, D. E. Staunton, and S. I. Simon Dynamic Regulation of LFA-1 Activation and Neutrophil Arrest on Intercellular Adhesion Molecule 1 (ICAM-1) in Shear Flow J. Biol. Chem., May 31, 2002; 277(23): 20660 - 20670. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. D. Becker, K. Garman, S. M. Whitcup, S. R. Planck, and J. T. Rosenbaum Inhibition of Leukocyte Sticking and Infiltration, but Not Rolling, by Antibodies to ICAM-1 and LFA-1 in Murine Endotoxin-Induced Uveitis Invest. Ophthalmol. Vis. Sci., October 1, 2001; 42(11): 2563 - 2566. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. B. Henderson, L. H.K. Lim, P. A. Tessier, F. N.E. Gavins, M. Mathies, M. Perretti, and N. Hogg The Use of Lymphocyte Function-Associated Antigen (Lfa)-1-Deficient Mice to Determine the Role of Lfa-1, Mac-1, and {alpha}4 Integrin in the Inflammatory Response of Neutrophils J. Exp. Med., July 16, 2001; 194(2): 219 - 226. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
