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Departments of Pathology and Cytometry, Cancer Research and Treatment Center, University of New Mexico Health Sciences Center, Albuquerque, NM 87131
The selective interaction of neutrophils with E-selectin and
eosinophils with P-selectin has been previously reported, but the
relevance of selectin site density and fluid shear has not been studied
in detail. We have developed a new approach to examine these
interactions in cell suspensions that integrates an on-line cone-plate
viscometer with a flow cytometer. We find that eosinophils and
neutrophils both use P-selectin glycoprotein ligand-1 to form stable
conjugates with P-selectin Chinese hamster ovary cell transfectants,
with a preferential adhesion of eosinophils. Further, the difference in
cell adhesion between neutrophils and eosinophils is magnified at
P-selectin expression levels below 
20 sites/µm2, a
range likely to be relevant to endothelial cell expression levels in
conditions associated with eosinophilia. The unique behavior is
retained over shear rates ranging from 100 to 1500/s but is magnified
at low shear. Results from parallel-plate flow chamber assays suggest
that preferential eosinophil adhesion reflects an enhanced efficiency
of initial PSGL-1 bond formation with P-selectin rather than a unique
ability of eosinophils to mediate rolling interactions of longer
duration on low-density P-selectin substrates. These differences may
account in part for the increase in eosinophil accumulation in allergic
diseases.
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