| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
CUTTING EDGE |
*
Division of Immune Regulation, La Jolla Institute for Allergy and Immunology, San Diego, CA 92121; and
Department of Microbiology and Molecular Genetics, University of California, Los Angeles, CA 90095
The choice of which determinants of a whole Ag will be presented on cell surface MHC class II molecules after uptake and processing by APC is the result of the interplay between structural characteristics of the Ag and the processing machinery of the APC. In this study, we demonstrate that introduction of a dibasic motif adjacent to a subdominant determinant enhances the presentation of this determinant from the whole molecule. This is the first report showing that a single amino acid substitution in a whole Ag, designed to introduce an endopeptidase recognition site, enhances display of class II-restricted determinants, most likely by creating a peptide chain cleavage in the antigenic molecule. Our findings have important implications for the understanding of immunodominance and for vaccine design.
This article has been cited by other articles:
|
|
 |
|
  J. M. Weaver, C. A. Lazarski, K. A. Richards, F. A. Chaves, S. A. Jenks, P. R. Menges, and A. J. Sant Immunodominance of CD4 T Cells to Foreign Antigens Is Peptide Intrinsic and Independent of Molecular Context: Implications for Vaccine Design J. Immunol., September 1, 2008; 181(5): 3039 - 3048. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Zhu, K. Liu, J. Cerny, T. Imoto, and K. D. Moudgil Insertion of the Dibasic Motif in the Flanking Region of a Cryptic Self-Determinant Leads to Activation of the Epitope-Specific T Cells J. Immunol., August 15, 2005; 175(4): 2252 - 2260. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Thai, G. Moine, M. Desmadril, D. Servent, J.-L. Tarride, A. Menez, and M. Leonetti Antigen Stability Controls Antigen Presentation J. Biol. Chem., November 26, 2004; 279(48): 50257 - 50266. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. P. Yeung, J. Chang, J. Miller, C. Barnett, M. Stickler, and F. A. Harding Elimination of an Immunodominant CD4+ T Cell Epitope in Human IFN-{beta} Does Not Result in an In Vivo Response Directed at the Subdominant Epitope J. Immunol., June 1, 2004; 172(11): 6658 - 6665. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. A. Braciak, B. Pedersen, J. Chin, C. Hsiao, E. S. Ward, I. Maricic, A. Jahng, F. L. Graham, J. Gauldie, E. E. Sercarz, et al. Protection Against Experimental Autoimmune Encephalomyelitis Generated by a Recombinant Adenovirus Vector Expressing the V{beta}8.2 TCR Is Disrupted by Coadministration with Vectors Expressing Either IL-4 or -10 J. Immunol., January 15, 2003; 170(2): 765 - 774. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Li, M. A. Haque, and J. S. Blum Role of Disulfide Bonds in Regulating Antigen Processing and Epitope Selection J. Immunol., September 1, 2002; 169(5): 2444 - 2450. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. A. Haque, P. Li, S. K. Jackson, H. M. Zarour, J. W. Hawes, U. T. Phan, M. Maric, P. Cresswell, and J. S. Blum Absence of {gamma}-Interferon-inducible Lysosomal Thiol Reductase in Melanomas Disrupts T Cell Recognition of Select Immunodominant Epitopes J. Exp. Med., May 20, 2002; 195(10): 1267 - 1277. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. J. Godkin, K. J. Smith, A. Willis, M. V. Tejada-Simon, J. Zhang, T. Elliott, and A. V. S. Hill Naturally Processed HLA Class II Peptides Reveal Highly Conserved Immunogenic Flanking Region Sequence Preferences That Reflect Antigen Processing Rather Than Peptide-MHC Interactions J. Immunol., June 1, 2001; 166(11): 6720 - 6727. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Carmicle, G. Dai, N. K. Steede, and S. J. Landry Proteolytic Sensitivity and Helper T-cell Epitope Immunodominance Associated with the Mobile Loop in Hsp10s J. Biol. Chem., January 4, 2002; 277(1): 155 - 160. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
