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*
Laboratory of Cellular and Molecular Immunology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892;
National Creative Research Initiative Center for Genetic Reprograming,
Institute for Molecular Biology and Genetics, and
§
Department of Molecular Biology, Seoul National University, Seoul, Korea; and
¶
Department of Oral Pathology, College of Dentistry, Kangreung National University, Kangneung, Korea
The gene LamC2 encoding the 
2 chain
of laminin 5, an epithelial cell-specific extracellular matrix protein,
was identified in a PCR-based subtracted cDNA library from mouse thymic
stromal cells. The mRNA existed in two alternative forms (5.1 and 2.4
kb). The full-length message was highly expressed in SCID thymus and in
a nurse cell line, but not in other thymic epithelial cell lines, while
the short form was more widely expressed. In situ
hybridization and immunohistochemical staining revealed laminin 5
expression mostly in the subcapsular region of the adult thymus.
Addition to fetal thymic organ cultures of a cell adhesion-blocking mAb
to the 
3 chain of laminin 5 interrupted T cell
development. There was a 40% reduction in the total yield of
thymocytes, and the most profound decrease (75–90%) was seen in the
CD25+CD44+ and
CD25+CD44-subsets of the
CD4-CD8- double negative fraction. Most of
the surviving double negative thymocytes expressed Sca-1, and there
were significant increases in the number of cells with CD69 expression
and in the fraction of annexin V-stained cells. None of these changes
were observed with a nonblocking anti-laminin 3
chain mAb. These results suggest that the interaction between double
negative thymoctyes and laminin 5 made by subcapsular epithelial cells
is required for the survival and differentiation of mouse
thymocytes.
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