| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Up-Regulate Substance P Receptor Expression in Murine Peritoneal Macrophages1
Department of Biology, University of North Carolina, Charlotte, NC 28223
While the ability of macrophages to express authentic substance P
receptors (i.e., NK-1 receptors) has been inferred from radioreceptor
binding assays and functional assays and, most recently, by
identification of NK-1 receptor mRNA expression, we know little about
NK-1 expression at the protein level or what host factors might
up-regulate expression of this receptor. In the present study we
demonstrate that the cytokines IL-4 and IFN-
can increase the
expression of NK-1 receptors on murine peritoneal macrophages.
Specifically, we show that IL-4 and IFN- can elicit increases in the
level of mRNA encoding the NK-1 receptor by up to 12- and 13-fold,
respectively. Furthermore, these cytokines can significantly increase
the expression of the NK-1 receptor protein as measured by Western blot
and FACS analysis using specific Abs developed in our laboratory. In
addition, we have demonstrated the ability of both IL-4 and IFN- to
enhance the ability of macrophages to bind substance P as measured by
radiolabeled binding assay. The observation that the level of
expression of this receptor protein can be enhanced by cytokines that
promote either cell-mediated (Th1) or humoral (Th2) immune responses
supports the idea that this receptor can be induced during either type
of immune response. As such, these results may point to a more
ubiquitous role for substance P in the generation of optimal immune
responses than previously appreciated.
This article has been cited by other articles:
|
|
 |
|
  N. J. Stevenson, M. R. Addley, E. J. Ryan, C. R. Boyd, H. P. Carroll, V. Paunovic, C. A. Bursill, H. C. Miller, K. M. Channon, A. E. McClurg, et al. CCL11 blocks IL-4 and GM-CSF signaling in hematopoietic cells and hinders dendritic cell differentiation via suppressor of cytokine signaling expression J. Leukoc. Biol., February 1, 2009; 85(2): 289 - 297. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Blum, T. Setiawan, L. Hang, K. Stoyanoff, and J. V. Weinstock Interleukin-12 (IL-12) and IL-23 Induction of Substance P Synthesis in Murine T Cells and Macrophages Is Subject to IL-10 and Transforming Growth Factor {beta} Regulation Infect. Immun., August 1, 2008; 76(8): 3651 - 3656. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Sun, R. D. Ramnath, L. Zhi, R. Tamizhselvi, and M. Bhatia Substance P enhances NF-{kappa}B transactivation and chemokine response in murine macrophages via ERK1/2 and p38 MAPK signaling pathways Am J Physiol Cell Physiol, June 1, 2008; 294(6): C1586 - C1596. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. A. Rettew, Y. M. Huet-Hudson, and I. Marriott Testosterone Reduces Macrophage Expression in the Mouse of Toll-Like Receptor 4, a Trigger for Inflammation and Innate Immunity Biol Reprod, March 1, 2008; 78(3): 432 - 437. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. D. Hazlett, S. A. McClellan, R. P. Barrett, J. Liu, Y. Zhang, and S. Lighvani Spantide I Decreases Type I Cytokines, Enhances IL-10, and Reduces Corneal Perforation in Susceptible Mice after Pseudomonas aeruginosa Infection Invest. Ophthalmol. Vis. Sci., February 1, 2007; 48(2): 797 - 807. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J.-P. Lai, W. Z. Ho, L. E. Kilpatrick, X. Wang, F. Tuluc, H. M. Korchak, and S. D. Douglas Full-length and truncated neurokinin-1 receptor expression and function during monocyte/macrophage differentiation PNAS, May 16, 2006; 103(20): 7771 - 7776. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Metwali, A. M. Blum, D. E. Elliott, T. Setiawan, and J. V. Weinstock Cutting Edge: Hemokinin Has Substance P-Like Function and Expression in Inflammation J. Immunol., June 1, 2004; 172(11): 6528 - 6532. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Rasley, I. Marriott, C. R. Halberstadt, K. L. Bost, and J. Anguita Substance P Augments Borrelia burgdorferi-Induced Prostaglandin E2 Production by Murine Microglia J. Immunol., May 1, 2004; 172(9): 5707 - 5713. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Bang, M. Biburger, W. L. Neuhuber, and G. Tiegs Neurokinin-1 Receptor Antagonists Protect Mice from CD95- and Tumor Necrosis Factor-{alpha}-Mediated Apoptotic Liver Damage J. Pharmacol. Exp. Ther., March 1, 2004; 308(3): 1174 - 1180. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. V. Weinstock, A. Blum, A. Metwali, D. Elliott, N. Bunnett, and R. Arsenescu Substance P Regulates Th1-Type Colitis in IL-10 Knockout Mice J. Immunol., October 1, 2003; 171(7): 3762 - 3767. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. R. Madrazo, S. L. Tranguch, and I. Marriott Signaling via Toll-Like Receptor 5 Can Initiate Inflammatory Mediator Production by Murine Osteoblasts Infect. Immun., September 1, 2003; 71(9): 5418 - 5421. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. P. M. van der Kleij, D. Ma, F. A. M. Redegeld, A. D. Kraneveld, F. P. Nijkamp, and J. Bienenstock Functional Expression of Neurokinin 1 Receptors on Mast Cells Induced by IL-4 and Stem Cell Factor J. Immunol., August 15, 2003; 171(4): 2074 - 2079. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Bang, G. Sass, A. K. Kiemer, A. M. Vollmar, W. L. Neuhuber, and G. Tiegs Neurokinin-1 Receptor Antagonists CP-96,345 and L-733,060 Protect Mice from Cytokine-Mediated Liver Injury J. Pharmacol. Exp. Ther., April 1, 2003; 305(1): 31 - 39. [Abstract] [Full Text]
|
|
|
|
|
|
S. F. Elsawa, W. Taylor, C. C. Petty, I. Marriott, J. V. Weinstock, and K. L. Bost Reduced CTL Response and Increased Viral Burden in Substance P Receptor-Deficient Mice Infected with Murine {gamma}-Herpesvirus 68 J. Immunol., March 1, 2003; 170(5): 2605 - 2612. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. A. McConnell, R. S. Yunus, S. A. Gross, K. L. Bost, M. G. Clemens, and F. M. Hughes Jr. Water Permeability of an Ovarian Antral Follicle Is Predominantly Transcellular and Mediated by Aquaporins Endocrinology, August 1, 2002; 143(8): 2905 - 2912. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Rameshwar, D. D. Joshi, P. Yadav, J. Qian, P. Gascon, V. T. Chang, D. Anjaria, J. S. Harrison, and X. Song Mimicry between neurokinin-1 and fibronectin may explain the transport and stability of increased substance P immunoreactivity in patients with bone marrow fibrosis Blood, May 15, 2001; 97(10): 3025 - 3031. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. M. BLUM, A. METWALI, C. CRAWFORD, J. LI, K. QADIR, D. E. ELLIOTT, and J. V. WEINSTOCK Interleukin 12 and antigen independently induce substance P receptor expression in T cells in murine schistosomiasis mansoni FASEB J, April 1, 2001; 15(6): 950 - 957. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
