| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Department of Experimental and Diagnostic Medicine, Section of General Pathology, and Center of Biotechnology, University of Ferrara, Ferrara, Italy
Endotoxin-dependent release of IL-1ß from mouse microglial cells is a very inefficient process, as it is slow and leads to accumulation of a modest amount of extracellular cytokine. Furthermore, secreted IL-1ß is mostly in the procytokine unprocessed form. Addition of extracellular ATP to LPS-primed microglia caused a burst of release of a large amount of processed IL-1ß. ATP had no effect on the accumulation of intracellular pro-IL-1ß in the absence of LPS. In LPS-treated cells, ATP slightly increased the synthesis of pro-IL-1ß. Optimal ATP concentration for IL-1ß secretion was between 3 and 5 mM, but significant release could be observed at concentrations as low as 1 mM. At all ATP concentrations IL-1ß release could be inhibited by increasing the extracellular K+ concentration. ATP-dependent IL-1ß release was also inhibited by 90 and 60% by the caspase inhibitors YVAD and DEVD, respectively. Accordingly, in ATP-stimulated microglia, the p20 proteolytic fragment derived from activation of the IL-1-ß-converting enzyme could be detected by immunoblot analysis. These experiments show that in mouse microglial cells extracellular ATP triggers fast maturation and release of intracellularly accumulated IL-ß by activating the IL-1-ß-converting enzyme/caspase 1.
This article has been cited by other articles:
|
|
 |
|
  N. Liu, A. A. Belperron, C. J. Booth, and L. K. Bockenstedt The Caspase 1 Inflammasome Is Not Required for Control of Murine Lyme Borreliosis Infect. Immun., August 1, 2009; 77(8): 3320 - 3327. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C.-W. Chiao, R. C. Tostes, and R. C. Webb P2X7 Receptor Activation Amplifies Lipopolysaccharide-Induced Vascular Hyporeactivity via Interleukin-1{beta} Release J. Pharmacol. Exp. Ther., September 1, 2008; 326(3): 864 - 870. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Myrtek, T. Muller, V. Geyer, N. Derr, D. Ferrari, G. Zissel, T. Durk, S. Sorichter, W. Luttmann, M. Kuepper, et al. Activation of Human Alveolar Macrophages via P2 Receptors: Coupling to Intracellular Ca2+ Increases and Cytokine Secretion J. Immunol., August 1, 2008; 181(3): 2181 - 2188. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Takenouchi, Y. Iwamaru, S. Sugama, M. Sato, M. Hashimoto, and H. Kitani Lysophospholipids and ATP Mutually Suppress Maturation and Release of IL-1{beta} in Mouse Microglial Cells Using a Rho-Dependent Pathway J. Immunol., June 15, 2008; 180(12): 7827 - 7839. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Franchi, T.-D. Kanneganti, G. R. Dubyak, and G. Nunez Differential Requirement of P2X7 Receptor and Intracellular K+ for Caspase-1 Activation Induced by Intracellular and Extracellular Bacteria J. Biol. Chem., June 29, 2007; 282(26): 18810 - 18818. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Burnstock Physiology and Pathophysiology of Purinergic Neurotransmission Physiol Rev, April 1, 2007; 87(2): 659 - 797. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Stock, T. Schilling, A. Schwab, and C. Eder Lysophosphatidylcholine Stimulates IL-1beta Release from Microglia via a P2X7 Receptor-Independent Mechanism J. Immunol., December 15, 2006; 177(12): 8560 - 8568. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Ferrari, C. Pizzirani, E. Adinolfi, R. M. Lemoli, A. Curti, M. Idzko, E. Panther, and F. Di Virgilio The P2X7 Receptor: A Key Player in IL-1 Processing and Release J. Immunol., April 1, 2006; 176(7): 3877 - 3883. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Burnstock Pathophysiology and therapeutic potential of purinergic signaling. Pharmacol. Rev., March 1, 2006; 58(1): 58 - 86. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. A. Verhoef, S. B. Kertesy, K. Lundberg, J. M. Kahlenberg, and G. R. Dubyak Inhibitory Effects of Chloride on the Activation of Caspase-1, IL-1{beta} Secretion, and Cytolysis by the P2X7 Receptor J. Immunol., December 1, 2005; 175(11): 7623 - 7634. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Sherwin and R. Fern Acute Lipopolysaccharide-Mediated Injury in Neonatal White Matter Glia: Role of TNF-{alpha}, IL-1{beta}, and Calcium J. Immunol., July 1, 2005; 175(1): 155 - 161. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Bianco, E. Pravettoni, A. Colombo, U. Schenk, T. Moller, M. Matteoli, and C. Verderio Astrocyte-Derived ATP Induces Vesicle Shedding and IL-1{beta} Release from Microglia J. Immunol., June 1, 2005; 174(11): 7268 - 7277. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Innocenti, S. Pfeiffer, E. Zrenner, K. Kohler, and E. Guenther ATP-Induced Non-Neuronal Cell Permeabilization in the Rat Inner Retina J. Neurosci., September 29, 2004; 24(39): 8577 - 8583. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Schilling and C. Eder A novel physiological mechanism of glycine-induced immunomodulation: Na+-coupled amino acid transporter currents in cultured brain macrophages J. Physiol., August 15, 2004; 559(1): 35 - 40. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. L. Wilson, S. E. Francis, S. K. Dower, and D. C. Crossman Secretion of Intracellular IL-1 Receptor Antagonist (Type 1) Is Dependent on P2X7 Receptor Activation J. Immunol., July 15, 2004; 173(2): 1202 - 1208. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Elssner, M. Duncan, M. Gavrilin, and M. D. Wewers A Novel P2X7 Receptor Activator, the Human Cathelicidin-Derived Peptide LL37, Induces IL-1{beta} Processing and Release J. Immunol., April 15, 2004; 172(8): 4987 - 4994. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Suzuki, I. Hide, K. Ido, S. Kohsaka, K. Inoue, and Y. Nakata Production and Release of Neuroprotective Tumor Necrosis Factor by P2X7 Receptor-Activated Microglia J. Neurosci., January 7, 2004; 24(1): 1 - 7. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Lee, S. Cha, M.-S. Lee, G. J. Cho, W. S. Choi, and K. Suk Role of Antiproliferative B Cell Translocation Gene-1 as an Apoptotic Sensitizer in Activation-Induced Cell Death of Brain Microglia J. Immunol., December 1, 2003; 171(11): 5802 - 5811. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Gudipaty, J. Munetz, P. A. Verhoef, and G. R. Dubyak Essential role for Ca2+ in regulation of IL-1{beta} secretion by P2X7 nucleotide receptor in monocytes, macrophages, and HEK-293 cells Am J Physiol Cell Physiol, August 1, 2003; 285(2): C286 - C299. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Morelli, P. Chiozzi, A. Chiesa, D. Ferrari, J. M. Sanz, S. Falzoni, P. Pinton, R. Rizzuto, M. F. Olson, and F. Di Virgilio Extracellular ATP Causes ROCK I-dependent Bleb Formation in P2X7-transfected HEK293 Cells Mol. Biol. Cell, July 1, 2003; 14(7): 2655 - 2664. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. K. Parvathenani, S. Tertyshnikova, C. R. Greco, S. B. Roberts, B. Robertson, and R. Posmantur P2X7 Mediates Superoxide Production in Primary Microglia and Is Up-regulated in a Transgenic Mouse Model of Alzheimer's Disease J. Biol. Chem., April 4, 2003; 278(15): 13309 - 13317. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. M. Labasi, N. Petrushova, C. Donovan, S. McCurdy, P. Lira, M. M. Payette, W. Brissette, J. R. Wicks, L. Audoly, and C. A. Gabel Absence of the P2X7 Receptor Alters Leukocyte Function and Attenuates an Inflammatory Response J. Immunol., June 15, 2002; 168(12): 6436 - 6445. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Chakfe, R. Seguin, J. P. Antel, C. Morissette, D. Malo, D. Henderson, and P. Seguela ADP and AMP Induce Interleukin-1beta Release from Microglial Cells through Activation of ATP-Primed P2X7 Receptor Channels J. Neurosci., April 15, 2002; 22(8): 3061 - 3069. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. A. Le Feuvre, D. Brough, Y. Iwakura, K. Takeda, and N. J. Rothwell Priming of Macrophages with Lipopolysaccharide Potentiates P2X7-mediated Cell Death via a Caspase-1-dependent Mechanism, Independently of Cytokine Production J. Biol. Chem., January 25, 2002; 277(5): 3210 - 3218. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Verderio and M. Matteoli ATP Mediates Calcium Signaling Between Astrocytes and Microglial Cells: Modulation by IFN-{{gamma}} J. Immunol., May 15, 2001; 166(10): 6383 - 6391. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Di Virgilio, P. Chiozzi, D. Ferrari, S. Falzoni, J. M. Sanz, A. Morelli, M. Torboli, G. Bolognesi, and O. R. Baricordi Nucleotide receptors: an emerging family of regulatory molecules in blood cells Blood, February 1, 2001; 97(3): 587 - 600. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Solle, J. Labasi, D. G. Perregaux, E. Stam, N. Petrushova, B. H. Koller, R. J. Griffiths, and C. A. Gabel Altered Cytokine Production in Mice Lacking P2X7 Receptors J. Biol. Chem., January 5, 2001; 276(1): 125 - 132. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. B. Mehta, J. Hart, and M. D. Wewers ATP-stimulated Release of Interleukin (IL)-1beta and IL-18 Requires Priming by Lipopolysaccharide and Is Independent of Caspase-1 Cleavage J. Biol. Chem., February 2, 2001; 276(6): 3820 - 3826. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Lee, J. Hur, P. Lee, J. Y. Kim, N. Cho, S. Y. Kim, H. Kim, M.-S. Lee, and K. Suk Dual Role of Inflammatory Stimuli in Activation-induced Cell Death of Mouse Microglial Cells. INITIATION OF TWO SEPARATE APOPTOTIC PATHWAYS VIA INDUCTION OF INTERFERON REGULATORY FACTOR-1 AND CASPASE-11 J. Biol. Chem., August 24, 2001; 276(35): 32956 - 32965. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
