The JI
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 QUICK SEARCH:   [advanced]


     
 


This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Request Permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Cerboni, C.
Right arrow Articles by Carbone, E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Cerboni, C.
Right arrow Articles by Carbone, E.
The Journal of Immunology, 2000, 164: 4775-4782.
Copyright © 2000 by The American Association of Immunologists

Human Cytomegalovirus Strain-Dependent Changes in NK Cell Recognition of Infected Fibroblasts1

Cristina Cerboni2,*, Mehrdad Mousavi-Jazi*,{dagger}, Annika Linde{dagger},{ddagger}, Kalle Söderström*, Maria Brytting*,{dagger}, Britta Wahren*,{dagger}, Klas Kärre* and Ennio Carbone*

* Microbiology and Tumor Biology Center, Karolinska Institute, Stockholm, Sweden; {dagger} Department of Virology, Swedish Institute for Infectious Disease Control, Stockholm, Sweden; and {ddagger} Department of Laboratory Medicine, Karolinska Hospital, Stockholm, Sweden

NK cells play a key role in the control of CMV infection in mice, but the mechanism by which NK cells can recognize and kill CMV-infected cells is unclear. In this study, the modulation of NK cell susceptibility of human CMV (hCMV)-infected cells was examined. We used a human lung and a human foreskin fibroblast cell line infected with clinical isolates (4636, 13B, or 109B) or with laboratory strains (AD169, Towne). The results indicate that all three hCMV clinical isolates confer a strong NK resistance, whereas only marginal or variable effects in the NK recognition were found when the laboratory strains were used. The same results were obtained regardless of the conditions of infection, effector cell activation status, cell culture conditions, and/or donor-target cell combinations. The NK cell inhibition did not correlate with HLA class I expression levels on the surface of the target cell and was independent of the leukocyte Ig-like receptor-1, as evaluated in Ab blocking experiments. No relevant changes were detected in the adhesion molecules ICAM-I and LFA-3 expressed on the cell surface of cells infected with hCMV clinical and laboratory strains. We conclude that hCMV possesses other mechanisms, related neither to target cell expression of HLA-I or adhesion molecules nor to NK cell expression of leukocyte Ig-like receptor-1, that confer resistance to NK cell recognition. Such mechanisms may be lost during in vitro passage of the virus. These results emphasize the differences between clinical hCMV isolates compared with laboratory strains.




This article has been cited by other articles:


Home page
Proc. Natl. Acad. Sci. USAHome page
Z. Yang and P. J. Bjorkman
Structure of UL18, a peptide-binding viral MHC mimic, bound to a host inhibitory receptor
PNAS, July 22, 2008; 105(29): 10095 - 10100.
[Abstract] [Full Text] [PDF]


Home page
J. Virol.Home page
R. J. Stanton, B. P. McSharry, C. R. Rickards, E. C. Y. Wang, P. Tomasec, and G. W. G. Wilkinson
Cytomegalovirus Destruction of Focal Adhesions Revealed in a High-Throughput Western Blot Analysis of Cellular Protein Expression
J. Virol., August 1, 2007; 81(15): 7860 - 7872.
[Abstract] [Full Text] [PDF]


Home page
J. Immunol.Home page
V. Prod'homme, C. Griffin, R. J. Aicheler, E. C. Y. Wang, B. P. McSharry, C. R. Rickards, R. J. Stanton, L. K. Borysiewicz, M. Lopez-Botet, G. W. G. Wilkinson, et al.
The Human Cytomegalovirus MHC Class I Homolog UL18 Inhibits LIR-1+ but Activates LIR-1- NK Cells
J. Immunol., April 1, 2007; 178(7): 4473 - 4481.
[Abstract] [Full Text] [PDF]


Home page
J. Virol.Home page
S. E. Chisholm and H. T. Reyburn
Recognition of Vaccinia Virus-Infected Cells by Human Natural Killer Cells Depends on Natural Cytotoxicity Receptors
J. Virol., March 1, 2006; 80(5): 2225 - 2233.
[Abstract] [Full Text] [PDF]


Home page
J. Immunol.Home page
M. R. Wills, O. Ashiru, M. B. Reeves, G. Okecha, J. Trowsdale, P. Tomasec, G. W. G. Wilkinson, J. Sinclair, and J. G. P. Sissons
Human Cytomegalovirus Encodes an MHC Class I-Like Molecule (UL142) That Functions to Inhibit NK Cell Lysis
J. Immunol., December 1, 2005; 175(11): 7457 - 7465.
[Abstract] [Full Text] [PDF]


Home page
J. Immunol.Home page
A.-C. Iversen, P. S. Norris, C. F. Ware, and C. A. Benedict
Human NK Cells Inhibit Cytomegalovirus Replication through a Noncytolytic Mechanism Involving Lymphotoxin-Dependent Induction of IFN-{beta}
J. Immunol., December 1, 2005; 175(11): 7568 - 7574.
[Abstract] [Full Text] [PDF]


Home page
J. Immunol.Home page
D. Saverino, F. Ghiotto, A. Merlo, S. Bruno, L. Battini, M. Occhino, M. Maffei, C. Tenca, S. Pileri, L. Baldi, et al.
Specific Recognition of the Viral Protein UL18 by CD85j/LIR-1/ILT2 on CD8+ T Cells Mediates the Non-MHC-Restricted Lysis of Human Cytomegalovirus-Infected Cells
J. Immunol., May 1, 2004; 172(9): 5629 - 5637.
[Abstract] [Full Text] [PDF]


Home page
J. Immunol.Home page
M. T. Barel, M. Ressing, N. Pizzato, D. van Leeuwen, P. Le Bouteiller, F. Lenfant, and E. J. H. J. Wiertz
Human Cytomegalovirus-Encoded US2 Differentially Affects Surface Expression of MHC Class I Locus Products and Targets Membrane-Bound, but Not Soluble HLA-G1 for Degradation
J. Immunol., December 15, 2003; 171(12): 6757 - 6765.
[Abstract] [Full Text] [PDF]


Home page
J. Immunol.Home page
A. Rolle, M. Mousavi-Jazi, M. Eriksson, J. Odeberg, C. Soderberg-Naucler, D. Cosman, K. Karre, and C. Cerboni
Effects of Human Cytomegalovirus Infection on Ligands for the Activating NKG2D Receptor of NK Cells: Up-Regulation of UL16-Binding Protein (ULBP)1 and ULBP2 Is Counteracted by the Viral UL16 Protein
J. Immunol., July 15, 2003; 171(2): 902 - 908.
[Abstract] [Full Text] [PDF]


Home page
J. Virol.Home page
J. Odeberg, H. Browne, S. Metkar, C. J. Froelich, L. Branden, D. Cosman, and C. Soderberg-Naucler
The Human Cytomegalovirus Protein UL16 Mediates Increased Resistance to Natural Killer Cell Cytotoxicity through Resistance to Cytolytic Proteins
J. Virol., April 15, 2003; 77(8): 4539 - 4545.
[Abstract] [Full Text] [PDF]


Home page
J. Virol.Home page
L. Rasmussen, A. Geissler, C. Cowan, A. Chase, and M. Winters
The Genes Encoding the gCIII Complex of Human Cytomegalovirus Exist in Highly Diverse Combinations in Clinical Isolates
J. Virol., October 2, 2002; 76(21): 10841 - 10848.
[Abstract] [Full Text] [PDF]


Home page
J. Immunol.Home page
C. S. Falk, M. Mach, D. J. Schendel, E. H. Weiss, I. Hilgert, and G. Hahn
NK Cell Activity During Human Cytomegalovirus Infection Is Dominated by US2-11-Mediated HLA Class I Down-Regulation
J. Immunol., September 15, 2002; 169(6): 3257 - 3266.
[Abstract] [Full Text] [PDF]


Home page
Proc. Natl. Acad. Sci. USAHome page
E. C. Y. Wang, B. McSharry, C. Retiere, P. Tomasec, S. Williams, L. K. Borysiewicz, V. M. Braud, and G. W. G. Wilkinson
From the Cover: UL40-mediated NK evasion during productive infection with human cytomegalovirus
PNAS, May 28, 2002; 99(11): 7570 - 7575.
[Abstract] [Full Text] [PDF]


Home page
J. Virol.Home page
J. Odeberg and C. Söderberg-Nauclér
Reduced Expression of HLA Class II Molecules and Interleukin-10- and Transforming Growth Factor {beta}1-Independent Suppression of T-Cell Proliferation in Human Cytomegalovirus-Infected Macrophage Cultures
J. Virol., June 1, 2001; 75(11): 5174 - 5181.
[Abstract] [Full Text]




HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Website Copyright © 2000 by The American Association of Immunologists, Inc. All rights reserved.
All Contents Copyright © 2000 by The American Association of Immunologists, Inc. All rights reserved.