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Cell Death-Associated Translocation of Plasma Membrane Components Induced by CTL¹

Yukishige Kawasaki^*, Takako Saito^*, Yoshiko Shirota-Someya, Yuko Ikegami, Hajime Komano, Mi-Heon Lee^2,, Christopher J. Froelich^§, Nobukata Shinohara^3, and Hajime Takayama^4,

^* Section for Bioimages, Division of Fundamental Research, Section for Cellular Immunology, Division of Pioneering Research, Project No.12, Project Research Center, Mitsubishi Kasei Institute of Life Sciences, Tokyo, Japan; and ^§ Department of Medicine, Evanston Northwestern Research Institute, Evanston, IL 60201

In the very early stages of target cell apoptosis induced by CTL, we found that fluorescence of labeling probes of the target plasma membrane, such as N-(3-triethylammoniumpropyl)-4-(p-dibutylaminostyryl)pyridinium dibromide (FM1-43), was translocated into intracellular membrane structures including nuclear envelope and mitochondria. This translocation was associated with the execution of CTL-mediated killing, because neither the CTL-target conjugation alone nor the binding of noncytotoxic Th2 clone with target cell was sufficient to provoke the process. Although FM1-43 translocation was observed in perforin-mediated cytotoxicity, examinations with several other dyes failed to detect the evidence for membrane damages that may cause influx of the dye. Moreover, the translocation was also observed in Fas-dependent apoptosis. These data indicate that the translocation precedes the damage of plasma membrane and intracellular organella in the course of apoptotic cell death and may represent the existence of a membrane trafficking that mediates the translocation of plasma membrane components in the early onset of apoptotic cell death.

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