HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Gerli, R. Articles by Lunardi, C. Search for Related Content PubMed PubMed Citation Articles by Gerli, R. Articles by Lunardi, C. Pubmed/NCBI databases Substance via MeSH Medline Plus Health Information Joint Disorders Rheumatoid Arthritis

CD30⁺ T Cells in Rheumatoid Synovitis: Mechanisms of Recruitment and Functional Role

Roberto Gerli^1,*, Costantino Pitzalis^¶, Onelia Bistoni^*, Brunangelo Falini, Vincenzo Costantini, Anna Russano^* and Claudio Lunardi^§

^* Department of Clinical and Experimental Medicine, Section of Internal Medicine and Oncological Sciences, Center for the Study of Rheumatic Diseases, Section of Hematology and Clinical Immunology, and Institute of Internal and Vascular Medicine, University of Perugia, Perugia, Italy; ^§ Department of Clinical and Experimental Medicine, Section of Internal Medicine, University of Verona, Verona, Italy; and ^¶ Department of Rheumatology, Division of Medicine, Guy’s and St. Thomas’ School of Medicine, London, United Kingdom

High serum levels of soluble CD30 (sCD30) have been reported to better predict the response to second line therapy in rheumatoid arthritis (RA). It is believed that sCD30 is released by CD30⁺ T cells present in the RA synovium. However, both the mechanism of recruitment to the joint and the functional role of this T cell subset in the pathogenesis of the disease remain unknown. This study confirmed higher levels of sCD30 in the serum and synovial fluid (SF) of RA patients compared with normal controls. However, analysis of mRNA and cell surface CD30 expression showed that CD30⁺ T cells are detectable in the SF, but not in the synovial membrane. In contrast, T cells expressing the CD30 transcript, but not the surface molecule, were found in the peripheral blood of both RA and normal controls. CD30 surface expression was up-regulated by adhesion and migration through endothelium in vitro and in a delayed-type hypersensitivity model in vivo. Although the great majority of fresh or cloned CD30⁺ T cells from SF produced both IFN- and IL-4, CD30 expression strictly correlated with IL-4 synthesis in synovial T cell clones. In addition, CD30⁺ T cell clones also produced high amounts of the anti-inflammatory cytokine IL-10. On this basis, we would like to propose that synovial CD30⁺ cells may play a role in the control of the inflammatory response. Serum sCD30 may reflect such cell activity and, therefore, explain the previously demonstrated correlation between high sCD30 serum levels and positive response to therapy.

This article has been cited by other articles:

				R. F.S. CARVALHO, A.-K. ULFGREN, M. ENGSTROM, E. a. KLINT, and G. NILSSON CD153 in Rheumatoid Arthritis: Detection of a Soluble Form in Serum and Synovial Fluid, and Expression by Mast Cells in the Rheumatic Synovium J Rheumatol, March 1, 2009; 36(3): 501 - 507. [Abstract] [Full Text] [PDF]

				R. Zeiser, V. H. Nguyen, J.-Z. Hou, A. Beilhack, E. Zambricki, M. Buess, C. H. Contag, and R. S. Negrin Early CD30 signaling is critical for adoptively transferred CD4+CD25+ regulatory T cells in prevention of acute graft-versus-host disease Blood, March 1, 2007; 109(5): 2225 - 2233. [Abstract] [Full Text] [PDF]

				C.-C. Su, H.-H. Chiu, C.-C. Chang, J.-C. Chen, and S.-M. Hsu CD30 Is Involved in Inhibition of T-Cell Proliferation by Hodgkin's Reed-Sternberg Cells1 Cancer Res., March 15, 2004; 64(6): 2148 - 2152. [Abstract] [Full Text] [PDF]

				J. A. Francisco, C. G. Cerveny, D. L. Meyer, B. J. Mixan, K. Klussman, D. F. Chace, S. X. Rejniak, K. A. Gordon, R. DeBlanc, B. E. Toki, et al. cAC10-vcMMAE, an anti-CD30-monomethyl auristatin E conjugate with potent and selective antitumor activity Blood, August 15, 2003; 102(4): 1458 - 1465. [Abstract] [Full Text] [PDF]

				R. Gerli, C. Lunardi, and C. Pitzalis Unmasking the anti-inflammatory cytokine response in rheumatoid synovitis Rheumatology, December 1, 2002; 41(12): 1341 - 1345. [Full Text] [PDF]

				A. F. Wahl, K. Klussman, J. D. Thompson, J. H. Chen, L. V. Francisco, G. Risdon, D. F. Chace, C. B. Siegall, and J. A. Francisco The Anti-CD30 Monoclonal Antibody SGN-30 Promotes Growth Arrest and DNA Fragmentation in Vitro and Affects Antitumor Activity in Models of Hodgkin's Disease Cancer Res., July 1, 2002; 62(13): 3736 - 3742. [Abstract] [Full Text] [PDF]

				J. F. Panus, C. A. Smith, C. A. Ray, T. D. Smith, D. D. Patel, and D. J. Pickup Cowpox virus encodes a fifth member of the tumor necrosis factor receptor family: A soluble, secreted CD30 homologue PNAS, June 11, 2002; 99(12): 8348 - 8353. [Abstract] [Full Text] [PDF]

				C. Susal, S. Pelzl, B. Dohler, and G. Opelz Identification of Highly Responsive Kidney Transplant Recipients Using Pretransplant Soluble CD30 J. Am. Soc. Nephrol., June 1, 2002; 13(6): 1650 - 1656. [Abstract] [Full Text] [PDF]

				A. Cerutti, A. Schaffer, R. G. Goodwin, S. Shah, H. Zan, S. Ely, and P. Casali Engagement of CD153 (CD30 Ligand) by CD30+ T Cells Inhibits Class Switch DNA Recombination and Antibody Production in Human IgD+ IgM+ B Cells J. Immunol., July 15, 2000; 165(2): 786 - 794. [Abstract] [Full Text] [PDF]