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Departments of
*
General and Experimental Pathology,
Pediatrics, and
Blood Group Serology, University of Vienna, Vienna, Austria; and
§
Children’s Hospital, Virchow Clinic, Humboldt University, Berlin, Germany
The prevalence of type I allergy to Hevea
brasiliensis latex is particularly high among individuals with
frequent exposure such as health care workers and patients with spina
bifida (SB). Due to a birth defect of the spinal canal and the
resulting neurological and orthopedic defects, these patients require
multiple surgeries during childhood. SB patients display a unique
pattern of sensitization: IgE-reactivity is preferentially directed
against Hev b 3 and Hev b 1, two latex allergens with high sequence
similarity. In this study, we analyzed the T cell response to Hev b 3
in latex-allergic SB patients using poly-, oligo-, and monoclonal T
lymphocyte cultures. All T cell clones (TCC) were CD3/CD4-positive and
expressed the 
ß TCR. According to their cytokine production
pattern (IL-4 vs IFN-
), 12 of 21 TCC were classified as Th2-like, 2
of 21 were Th1-like, and 7 of 21 belonged to a Th0-like subset. Using
11 T cell lines and 21 TCC, nine T cell stimulating fragments were
determined out of 52 overlapping 12-mer peptides representing the
complete amino acid sequence of Hev b 3. Ag presentation of one
dominant T cell epitope could be associated with a four-amino acid
binding motif (YSTS, position 11–13) in the ß1 chain of HLA-DR
molecules expressed by the respective patients. No reactivity was
observed when Hev b 3-reactive T cell lines or TCC were incubated with
peptides representing homologous parts of the Hev b 1 molecule, i.e.,
no cross-reactivity between Hev b 3 and Hev b 1 at the T cell level was
evident.
This article has been cited by other articles:
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B. Bohle, L. Orel, D. Kraft, and C. Ebner Oligodeoxynucleotides Containing CpG Motifs Induce Low Levels of TNF-{{alpha}} in Human B Lymphocytes: Possible Adjuvants for Th1 Responses J. Immunol., March 15, 2001; 166(6): 3743 - 3748. [Abstract] [Full Text] [PDF]
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