| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
*
Virginia Mason Research Center, Seattle, WA 98101;
R. H. Williams Laboratory and Molecular and Cellular Biology Program and
Department of Immunology, University of Washington, Seattle, WA 98195; and
§
Virology Division, Harborview Medical Center, Seattle, WA 98104
Ag-specific CD4+ T cells are present in peripheral blood in low frequency, where they undergo recruitment and expansion during immune responses and in the pathogenesis of numerous autoimmune diseases. MHC tetramers, which constitute a labeled MHC-peptide ligand suitable for binding to the Ag-specific receptor on T cells, provide a novel approach for the detection and characterization of such rare cells. In this study, we utilized this technology to identify HLA DQ-restricted Ag-specific T cells in the peripheral blood of human subjects and to identify immunodominant epitopes associated with viral infection. Peptides representing potential epitope regions of the VP16 protein from HSV-2 were loaded onto recombinant DQ0602 molecules to generate a panel of Ag-specific DQ0602 tetramers. VP16 Ag-specific DQ-restricted T cells were identified and expanded from the peripheral blood of HSV-2-infected individuals, representing two predominant epitope specificities. Although the VP16 369–380 peptide has a lower binding affinity for DQ0602 molecules than the VP16 33–52 peptide, T cells that recognized the VP16 369–380 peptide occurred at a much higher frequency than those that were specific for the VP16 33–52 peptide.
This article has been cited by other articles:
|
|
 |
|
  E. A. James, J. Bui, D. Berger, L. Huston, M. Roti, and W. W. Kwok Tetramer-guided epitope mapping reveals broad, individualized repertoires of tetanus toxin-specific CD4+ T cells and suggests HLA-based differences in epitope recognition Int. Immunol., November 1, 2007; 19(11): 1291 - 1301. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Bioley, C. Jandus, S. Tuyaerts, D. Rimoldi, W. W. Kwok, D. E. Speiser, J.-M. Tiercy, K. Thielemans, J.-C. Cerottini, and P. Romero Melan-A/MART-1-Specific CD4 T Cells in Melanoma Patients: Identification of New Epitopes and Ex Vivo Visualization of Specific T Cells by MHC Class II Tetramers J. Immunol., November 15, 2006; 177(10): 6769 - 6779. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. M. Koelle, J. Huang, M. T. Hensel, and C. L. McClurkan Innate Immune Responses to Herpes Simplex Virus Type 2 Influence Skin Homing Molecule Expression by Memory CD4+ Lymphocytes J. Virol., March 15, 2006; 80(6): 2863 - 2872. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Kinnunen, W. W. Kwok, A. Narvanen, M. Rytkonen-Nissinen, A. Immonen, S. Saarelainen, A. Taivainen, and T. Virtanen Immunomodulatory potential of heteroclitic analogs of the dominant T-cell epitope of lipocalin allergen Bos d 2 on specific T cells Int. Immunol., December 1, 2005; 17(12): 1573 - 1581. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Giannoni, J. Barnett, K. Bi, R. Samodal, P. Lanza, P. Marchese, R. Billetta, R. Vita, M. R. Klein, B. Prakken, et al. Clustering of T Cell Ligands on Artificial APC Membranes Influences T Cell Activation and Protein Kinase C {theta} Translocation to the T Cell Plasma Membrane J. Immunol., March 15, 2005; 174(6): 3204 - 3211. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. M. S. Huang, P. Borszcz, S. Sidobre, M. Kronenberg, and K. P. Kane CD1d1 Displayed on Cell Size Beads Identifies and Enriches an NK Cell Population Negatively Regulated by CD1d1 J. Immunol., May 1, 2004; 172(9): 5304 - 5312. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Landais, X. Saulquin, E. Scotet, L. Trautmann, M.-A. Peyrat, J. L. Yates, W. W. Kwok, M. Bonneville, and E. Houssaint Direct killing of Epstein-Barr virus (EBV)-infected B cells by CD4 T cells directed against the EBV lytic protein BHRF1 Blood, February 15, 2004; 103(4): 1408 - 1416. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. A. Danke and W. W. Kwok HLA Class II-Restricted CD4+ T Cell Responses Directed Against Influenza Viral Antigens Postinfluenza Vaccination J. Immunol., September 15, 2003; 171(6): 3163 - 3169. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
U. Holzer, W. W. Kwok, G. T. Nepom, and J. H. Buckner Differential Antigen Sensitivity and Costimulatory Requirements in Human Th1 and Th2 Antigen-Specific CD4+ Cells with Similar TCR Avidity J. Immunol., February 1, 2003; 170(3): 1218 - 1223. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Reddy, E. Bettelli, L. Nicholson, H. Waldner, M.-H. Jang, K. W. Wucherpfennig, and V. K. Kuchroo Detection of Autoreactive Myelin Proteolipid Protein 139-151-Specific T Cells by Using MHC II (IAs) Tetramers J. Immunol., January 15, 2003; 170(2): 870 - 877. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. M. Koelle and L. Corey Recent Progress in Herpes Simplex Virus Immunobiology and Vaccine Research Clin. Microbiol. Rev., January 1, 2003; 16(1): 96 - 113. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. I. Mallet-Designe, T. Stratmann, D. Homann, F. Carbone, M. B. A. Oldstone, and L. Teyton Detection of Low-Avidity CD4+ T Cells Using Recombinant Artificial APC: Following the Antiovalbumin Immune Response J. Immunol., January 1, 2003; 170(1): 123 - 131. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Sidney, M.-F. del Guercio, S. Southwood, and A. Sette The HLA Molecules DQA1*0501/B1*0201 and DQA1*0301/B1*0302 Share an Extensive Overlap in Peptide Binding Specificity J. Immunol., November 1, 2002; 169(9): 5098 - 5108. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Schepers, M. Toebes, G. Sotthewes, F. A. Vyth-Dreese, T. A. M. Dellemijn, C. J. M. Melief, F. Ossendorp, and T. N. M. Schumacher Differential Kinetics of Antigen-Specific CD4+ and CD8+ T Cell Responses in the Regression of Retrovirus-Induced Sarcomas J. Immunol., September 15, 2002; 169(6): 3191 - 3199. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Reijonen, E. J. Novak, S. Kochik, A. Heninger, A. W. Liu, W. W. Kwok, and G. T. Nepom Detection of GAD65-Specific T-Cells by Major Histocompatibility Complex Class II Tetramers in Type 1 Diabetic Patients and At-Risk Subjects Diabetes, May 1, 2002; 51(5): 1375 - 1382. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. H. J. Vogt, J. W. van den Muijsenberg, E. Goulmy, E. Spierings, P. Kluck, M. G. Kester, R. A. van Soest, J. W. Drijfhout, R. Willemze, and J. H. F. Falkenburg The DBY gene codes for an HLA-DQ5-restricted human male-specific minor histocompatibility antigen involved in graft-versus-host disease Blood, April 15, 2002; 99(8): 3027 - 3032. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. F. Greten and J. P. Schneck Development and Use of Multimeric Major Histocompatibility Complex Molecules Clin. Vaccine Immunol., March 1, 2002; 9(2): 216 - 220. [Full Text] [PDF]
|
|
|
|
|
|
H. Quarsten, S. N. McAdam, T. Jensen, H. Arentz-Hansen, K. E. A. Lundin, and L. M. Sollid Staining of Celiac Disease-Relevant T Cells by Peptide-DQ2 Multimers J. Immunol., November 1, 2001; 167(9): 4861 - 4868. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. J. Novak, S. A. Masewicz, A. W. Liu, A. Lernmark, W. W. Kwok, and G. T. Nepom Activated human epitope-specific T cells identified by class II tetramers reside within a CD4high, proliferating subset Int. Immunol., June 1, 2001; 13(6): 799 - 806. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. J. Novak, A. W. Liu, J. A. Gebe, B. A. Falk, G. T. Nepom, D. M. Koelle, and W. W. Kwok Tetramer-Guided Epitope Mapping: Rapid Identification and Characterization of Immunodominant CD4+ T Cell Epitopes from Complex Antigens J. Immunol., June 1, 2001; 166(11): 6665 - 6670. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Y. Braun, I. Grandjean, P. Feunou, L. Duban, R. Kiss, M. Goldman, and O. Lantz Acute Rejection in the Absence of Cognate Recognition of Allograft by T Cells J. Immunol., April 15, 2001; 166(8): 4879 - 4883. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. O. Cameron, J. R. Cochran, B. Yassine-Diab, R.-P. Sekaly, and L. J. Stern Cutting Edge: Detection of Antigen-Specific CD4+ T Cells by HLA-DR1 Oligomers Is Dependent on the T Cell Activation State J. Immunol., January 15, 2001; 166(2): 741 - 745. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Reichstetter, R. A. Ettinger, A. W. Liu, J. A. Gebe, G. T. Nepom, and W. W. Kwok Distinct T Cell Interactions with HLA Class II Tetramers Characterize a Spectrum of TCR Affinities in the Human Antigen-Specific T Cell Response J. Immunol., December 15, 2000; 165(12): 6994 - 6998. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. M. Koelle, M. Schomogyi, C. McClurkan, S. N. Reymond, and H. B. Chen CD4 T-Cell Responses to Herpes Simplex Virus Type 2 Major Capsid Protein VP5: Comparison with Responses to Tegument and Envelope Glycoproteins J. Virol., December 1, 2000; 74(23): 11422 - 11425. [Abstract] [Full Text]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
