| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Department of Microbiology and Immunology, Emory University School of Medicine, Atlanta, GA 30322
Inhibition of class II trans-activator (CIITA)
expression prevents embryonic trophoblast cells from up-regulating MHC
class II genes in response to IFN-
. This is thought to be one
mechanism of maternal tolerance to the fetal allograft. The CIITA gene
is regulated by four distinct promoters; promoter III directs
constitutive (B cell) expression, and promoter IV regulates
IFN--inducible expression. Using in vivo genomic footprinting,
promoter-reporter analysis, Southern blot analysis, and RT-PCR, we have
examined the cause of CIITA silencing in a trophoblast-derived cell
line. We report here that methylation of promoter IV DNA at CpG sites
in Jar cells prevents promoter occupancy and IFN--inducible
transcription. The inhibition of CpG methylation in Jar cells by
treatment with 5-aza-2'-deoxycytidine restores IFN- inducibility to
CIITA. This is the first description of an epigenetic mechanism
involved in regulation of CIITA and MHC class II gene
expression.
This article has been cited by other articles:
|
|
 |
|
  A. De Lerma Barbaro, A. De Ambrosis, B. Banelli, G. L. Pira, O. Aresu, M. Romani, S. Ferrini, and R. S. Accolla Methylation of CIITA promoter IV causes loss of HLA-II inducibility by IFN-{gamma} in promyelocytic cells Int. Immunol., November 1, 2008; 20(11): 1457 - 1466. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. M. Holling, M. W. T. Bergevoet, L. Wilson, M. C. J. A. Van Eggermond, E. Schooten, R. D. M. Steenbergen, P. J. F. Snijders, M. J. Jager, and P. J. Van den Elsen A Role for EZH2 in Silencing of IFN-{gamma} Inducible MHC2TA Transcription in Uveal Melanoma J. Immunol., October 15, 2007; 179(8): 5317 - 5325. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S.-D. Chou, A. N. H. Khan, W. J. Magner, and T. B. Tomasi Histone acetylation regulates the cell type specific CIITA promoters, MHC class II expression and antigen presentation in tumor cells Int. Immunol., November 1, 2005; 17(11): 1483 - 1494. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
U. M. Nagarajan, D. M. Ojcius, L. Stahl, R. G. Rank, and T. Darville Chlamydia trachomatis Induces Expression of IFN-{gamma}-Inducible Protein 10 and IFN-{beta} Independent of TLR2 and TLR4, but Largely Dependent on MyD88 J. Immunol., July 1, 2005; 175(1): 450 - 460. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Radosevich, Z. Song, J. C. Gorga, B. Ksander, and S. J. Ono Epigenetic Silencing of the CIITA Gene and Posttranscriptional Regulation of Class II MHC Genes in Ocular Melanoma Cells Invest. Ophthalmol. Vis. Sci., September 1, 2004; 45(9): 3185 - 3195. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
U. M. Nagarajan, A. B. Long, M. T. Harreman, A. H. Corbett, and J. M. Boss A Hierarchy of Nuclear Localization Signals Governs the Import of the Regulatory Factor X Complex Subunits and MHC Class II Expression J. Immunol., July 1, 2004; 173(1): 410 - 419. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. M. Holling, E. Schooten, A. W. Langerak, and P. J. van den Elsen Regulation of MHC class II expression in human T-cell malignancies Blood, February 15, 2004; 103(4): 1438 - 1444. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Holtz, J. C. Choi, M. G. Petroff, J. F. Piskurich, and S. P. Murphy Class II Transactivator (CIITA) Promoter Methylation Does Not Correlate with Silencing of CIITA Transcription in Trophoblasts Biol Reprod, September 1, 2003; 69(3): 915 - 924. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. K. Pai, M. Convery, T. A. Hamilton, W. H. Boom, and C. V. Harding Inhibition of IFN-{gamma}-Induced Class II Transactivator Expression by a 19-kDa Lipoprotein from Mycobacterium tuberculosis: A Potential Mechanism for Immune Evasion J. Immunol., July 1, 2003; 171(1): 175 - 184. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. J. van den Elsen, N. van der Stoep, and T. Yazawa Class II Transactivator (CIITA) Deficiency in Tumor Cells: Complicated Mechanisms or Not? Am. J. Pathol., July 1, 2003; 163(1): 373 - 376. [Full Text] [PDF]
|
|
|
|
|
|
T. Kanaseki, H. Ikeda, Y. Takamura, M. Toyota, Y. Hirohashi, T. Tokino, T. Himi, and N. Sato Histone Deacetylation, But Not Hypermethylation, Modifies Class II Transactivator and MHC Class II Gene Expression in Squamous Cell Carcinomas J. Immunol., May 15, 2003; 170(10): 4980 - 4985. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Moreau, G. Mouillot, P. Rousseau, C. Marcou, J. Dausset, and E. D. Carosella HLA-G gene repression is reversed by demethylation PNAS, February 4, 2003; 100(3): 1191 - 1196. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. van der Stoep, E. Quinten, and P. J. van den Elsen Transcriptional Regulation of the MHC Class II Trans-Activator (CIITA) Promoter III: Identification of a Novel Regulatory Region in the 5'-Untranslated Region and an Important Role for cAMP-Responsive Element Binding Protein 1 and Activating Transcription Factor-1 in CIITA-Promoter III Transcriptional Activation in B Lymphocytes J. Immunol., November 1, 2002; 169(9): 5061 - 5071. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. P. Murphy, R. Holtz, N. Lewandowski, T. B. Tomasi, and H. Fuji DNA Alkylating Agents Alleviate Silencing of Class II Transactivator Gene Expression in L1210 Lymphoma Cells J. Immunol., September 15, 2002; 169(6): 3085 - 3093. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. K. Pai, D. Askew, W. H. Boom, and C. V. Harding Regulation of Class II MHC Expression in APCs: Roles of Types I, III, and IV Class II Transactivator J. Immunol., August 1, 2002; 169(3): 1326 - 1333. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. C. Morris, G. W. Beresford, M. R. Mooney, and J. M. Boss Kinetics of a Gamma Interferon Response: Expression and Assembly of CIITA Promoter IV and Inhibition by Methylation Mol. Cell. Biol., July 1, 2002; 22(13): 4781 - 4791. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Naves, A. M. Lennon, G. Barbieri, L. Reyes, G. Puga, L. Salas, V. Deffrennes, M. Rosemblatt, M. Fellous, D. Charron, et al. MHC class II-deficient tumor cell lines with a defective expression of the class II transactivator Int. Immunol., May 1, 2002; 14(5): 481 - 491. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J.-M. Waldburger, T. Suter, A. Fontana, H. Acha-Orbea, and W. Reith Selective Abrogation of Major Histocompatibility Complex Class II Expression on Extrahematopoietic Cells in Mice Lacking Promoter IV of the Class II Transactivator Gene J. Exp. Med., August 20, 2001; 194(4): 393 - 406. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. J. Magner, A. L. Kazim, C. Stewart, M. A. Romano, G. Catalano, C. Grande, N. Keiser, F. Santaniello, and T. B. Tomasi Activation of MHC Class I, II, and CD40 Gene Expression by Histone Deacetylase Inhibitors J. Immunol., December 15, 2000; 165(12): 7017 - 7024. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
