The JI
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     
 

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Request Permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Banerjee, R.
Right arrow Articles by Fikrig, E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Banerjee, R.
Right arrow Articles by Fikrig, E.
The Journal of Immunology, 2000, 164: 3946-3949.
Copyright © 2000 by The American Association of Immunologists

CUTTING EDGE

Cutting Edge: Infection by the Agent of Human Granulocytic Ehrlichiosis Prevents the Respiratory Burst by Down-Regulating gp91phox1

Rila Banerjee*, Juan Anguita*, Dirk Roos{dagger} and Erol Fikrig2,*

* Section of Rheumatology, Department of Internal Medicine, Yale University School of Medicine, New Haven, CT 06520; and {dagger} Central Laboratory of the Netherlands Blood Transfusion Service and Laboratory for Experimental and Clinical Immunology, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands.

The agent of human granulocytic ehrlichiosis (HGE) is an emerging tick-borne pathogen that resides in neutrophils and can be cultured in a promyelocytic (HL-60) cell line. In response to microbes, polymorphonuclear leukocytes normally activate the NADPH oxidase enzyme complex and generate superoxide anion (O2-). However, HL-60 cells infected with HGE bacteria did not produce O2- upon activation with PMA. RT-PCR demonstrated that HGE organisms inhibited mRNA expression of a single component of NADPH oxidase, gp91phox, and FACS analysis showed that plasma membrane-associated gp91phox protein was reduced on the infected cells. Infection with HGE organisms also decreased gp91phox mRNA levels in splenic neutrophils in a murine model of HGE, demonstrating this phenomenon in vivo. Therefore, HGE bacteria repress the respiratory burst by down-regulating gp91phox, the first direct inhibition of NADPH oxidase by a pathogen.




This article has been cited by other articles:


Home page
 Infect. Immun.Home page
V. Thomas, S. Samanta, and E. Fikrig
Anaplasma phagocytophilum Increases Cathepsin L Activity, Thereby Globally Influencing Neutrophil Function
Infect. Immun., November 1, 2008; 76(11): 4905 - 4912.
[Abstract] [Full Text] [PDF]

Home page
 CVIHome page
D. G. Scorpio, F. D. von Loewenich, H. Gobel, C. Bogdan, and J. S. Dumler
Innate Immune Response to Anaplasma phagocytophilum Contributes to Hepatic Injury.
Clin. Vaccine Immunol., July 1, 2006; 13(7): 806 - 809.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
E. Yager, C. Bitsaktsis, B. Nandi, J. W. McBride, and G. Winslow
Essential Role for Humoral Immunity during Ehrlichia Infection in Immunocompetent Mice
Infect. Immun., December 1, 2005; 73(12): 8009 - 8016.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
B. Sukumaran, J. A. Carlyon, J.-L. Cai, N. Berliner, and E. Fikrig
Early Transcriptional Response of Human Neutrophils to Anaplasma phagocytophilum Infection
Infect. Immun., December 1, 2005; 73(12): 8089 - 8099.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
N.-K. Pham, J. Mouriz, and P. E. Kima
Leishmania pifanoi Amastigotes Avoid Macrophage Production of Superoxide by Inducing Heme Degradation
Infect. Immun., December 1, 2005; 73(12): 8322 - 8333.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
J. A. Carlyon, D. Ryan, K. Archer, and E. Fikrig
Effects of Anaplasma phagocytophilum on Host Cell Ferritin mRNA and Protein Levels
Infect. Immun., November 1, 2005; 73(11): 7629 - 7636.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
J. R. Abbott, G. H. Palmer, K. A. Kegerreis, P. F. Hetrick, C. J. Howard, J. C. Hope, and W. C. Brown
Rapid and Long-Term Disappearance of CD4+ T Lymphocyte Responses Specific for Anaplasma Marginale Major Surface Protein-2 (MSP2) in MSP2 Vaccinates following Challenge with Live A. marginale
J. Immunol., June 1, 2005; 174(11): 6702 - 6715.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
D. L. Borjesson, S. D. Kobayashi, A. R. Whitney, J. M. Voyich, C. M. Argue, and F. R. DeLeo
Insights into Pathogen Immune Evasion Mechanisms: Anaplasma phagocytophilum Fails to Induce an Apoptosis Differentiation Program in Human Neutrophils
J. Immunol., May 15, 2005; 174(10): 6364 - 6372.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
J. W. A. Garyu, K.-s. Choi, D. J. Grab, and J. S. Dumler
Defective Phagocytosis in Anaplasma phagocytophilum- Infected Neutrophils
Infect. Immun., February 1, 2005; 73(2): 1187 - 1190.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
V. Thomas, S. Samanta, C. Wu, N. Berliner, and E. Fikrig
Anaplasma phagocytophilum Modulates gp91phox Gene Expression through Altered Interferon Regulatory Factor 1 and PU.1 Levels and Binding of CCAAT Displacement Protein
Infect. Immun., January 1, 2005; 73(1): 208 - 218.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
J. W. IJdo and A. C. Mueller
Neutrophil NADPH Oxidase Is Reduced at the Anaplasma phagocytophilum Phagosome
Infect. Immun., September 1, 2004; 72(9): 5392 - 5401.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
J. A. Carlyon, D. A. Latif, M. Pypaert, P. Lacy, and E. Fikrig
Anaplasma phagocytophilum Utilizes Multiple Host Evasion Mechanisms To Thwart NADPH Oxidase-Mediated Killing during Neutrophil Infection
Infect. Immun., August 1, 2004; 72(8): 4772 - 4783.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
S. Tsunawaki, L. S. Yoshida, S. Nishida, T. Kobayashi, and T. Shimoyama
Fungal Metabolite Gliotoxin Inhibits Assembly of the Human Respiratory Burst NADPH Oxidase
Infect. Immun., June 1, 2004; 72(6): 3373 - 3382.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
K.-s. Choi, D. J. Grab, and J. S. Dumler
Anaplasma phagocytophilum Infection Induces Protracted Neutrophil Degranulation
Infect. Immun., June 1, 2004; 72(6): 3680 - 3683.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
J. Park, K.-S. Choi, D. J. Grab, and J. S. Dumler
Divergent Interactions of Ehrlichia chaffeensis- and Anaplasma phagocytophilum-Infected Leukocytes with Endothelial Cell Barriers
Infect. Immun., December 1, 2003; 71(12): 6728 - 6733.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
J. A. Carlyon, M. Akkoyunlu, L. Xia, T. Yago, T. Wang, R. D. Cummings, R. P. McEver, and E. Fikrig
Murine neutrophils require {alpha}1,3-fucosylation but not PSGL-1 for productive infection with Anaplasma phagocytophilum
Blood, November 1, 2003; 102(9): 3387 - 3395.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
M. Lin and Y. Rikihisa
Ehrlichia chaffeensis and Anaplasma phagocytophilum Lack Genes for Lipid A Biosynthesis and Incorporate Cholesterol for Their Survival
Infect. Immun., September 1, 2003; 71(9): 5324 - 5331.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
K.-S. Choi, J. Garyu, J. Park, and J. S. Dumler
Diminished Adhesion of Anaplasma phagocytophilum-Infected Neutrophils to Endothelial Cells Is Associated with Reduced Expression of Leukocyte Surface Selectin
Infect. Immun., August 1, 2003; 71(8): 4586 - 4594.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
H. Scaife, Z. Woldehiwet, C. A. Hart, and S. W. Edwards
Anaplasma phagocytophilum Reduces Neutrophil Apoptosis In Vivo
Infect. Immun., April 1, 2003; 71(4): 1995 - 2001.
[Abstract] [Full Text]

Home page
 J. Immunol.Home page
J. A. Carlyon, W.-T. Chan, J. Galan, D. Roos, and E. Fikrig
Repression of rac2 mRNA Expression by Anaplasma phagocytophila Is Essential to the Inhibition of Superoxide Production and Bacterial Proliferation
J. Immunol., December 15, 2002; 169(12): 7009 - 7018.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
J. W. IJdo, C. Wu, S. R. Telford III, and E. Fikrig
Differential Expression of the p44 Gene Family in the Agent of Human Granulocytic Ehrlichiosis
Infect. Immun., September 1, 2002; 70(9): 5295 - 5298.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
J. Mott, Y. Rikihisa, and S. Tsunawaki
Effects of Anaplasma phagocytophila on NADPH Oxidase Components in Human Neutrophils and HL-60 Cells
Infect. Immun., March 1, 2002; 70(3): 1359 - 1366.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
R. R. Ganta, M. J. Wilkerson, C. Cheng, A. M. Rokey, and S. K. Chapes
Persistent Ehrlichia chaffeensis Infection Occurs in the Absence of Functional Major Histocompatibility Complex Class II Genes
Infect. Immun., January 1, 2002; 70(1): 380 - 388.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
M. Akkoyunlu, S. E. Malawista, J. Anguita, and E. Fikrig
Exploitation of Interleukin-8-Induced Neutrophil Chemotaxis by the Agent of Human Granulocytic Ehrlichiosis
Infect. Immun., September 1, 2001; 69(9): 5577 - 5588.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
J. Mott and Y. Rikihisa
Human Granulocytic Ehrlichiosis Agent Inhibits Superoxide Anion Generation by Human Neutrophils
Infect. Immun., December 1, 2000; 68(12): 6697 - 6703.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
R. Banerjee, J. Anguita, and E. Fikrig
Granulocytic Ehrlichiosis in Mice Deficient in Phagocyte Oxidase or Inducible Nitric Oxide Synthase
Infect. Immun., July 1, 2000; 68(7): 4361 - 4362.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Website Copyright © 2000 by The American Association of Immunologists, Inc. All rights reserved.
All Contents Copyright © 2000 by The American Association of Immunologists, Inc. All rights reserved.