HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Saifuddin, M. Articles by Spear, G. T. Search for Related Content PubMed PubMed Citation Articles by Saifuddin, M. Articles by Spear, G. T.

Cutting Edge: Activation of HIV-1 Transcription by the MHC Class II Transactivator¹

Mohammed Saifuddin^2,*, Kenneth A. Roebuck^*, Cheong-hee Chang, Jenny P. Y. Ting and Gregory T. Spear^*

^* Department of Immunology/Microbiology, Rush University, Chicago, IL 60612; Department of Microbiology and Immunology, University of Michigan, Ann Arbor, MI 48109; and Department of Microbiology and Immunology, Lineberger Comprehensive Cancer Center, University of North Carolina, Chapel Hill, NC 27514

Both macrophages and activated CD4⁺ T cells can be productively infected by HIV-1, and both cell types express MHC class II molecules. Expression of MHC class II proteins in these cells is regulated by a specific transcriptional coactivator, the class II transactivator (CIITA). In this study, we report for the first time that CIITA expression profoundly influences HIV-1 replication. Stable expression of CIITA in Jurkat cells markedly increased 1) HIV-1 replication as assessed by the p24 Ag production and 2) luciferase expression after transfection with full-length provirus or long terminal repeat constructs. Similarly, transient expression of CIITA increased provirus expression as well as long terminal repeat promoter activity in 293 and HeLa-T4 cells. In contrast, mutant forms of CIITA did not increase HIV-1 expression. This study shows that expression of CIITA increases HIV-1 replication through a transcriptional mechanism.

This article has been cited by other articles:

				T. M. Holling, E. Schooten, A. W. Langerak, and P. J. van den Elsen Regulation of MHC class II expression in human T-cell malignancies Blood, February 15, 2004; 103(4): 1438 - 1444. [Abstract] [Full Text] [PDF]

				T. S. Gourley, D. R. Patel, K. Nickerson, S.-C. Hong, and C.-H. Chang Aberrant Expression of Fas Ligand in Mice Deficient for the MHC Class II Transactivator J. Immunol., May 1, 2002; 168(9): 4414 - 4419. [Abstract] [Full Text] [PDF]

				T. M. Holling, N. van der Stoep, E. Quinten, and P. J. van den Elsen Activated Human T Cells Accomplish MHC Class II Expression Through T Cell-Specific Occupation of Class II Transactivator Promoter III J. Immunol., January 15, 2002; 168(2): 763 - 770. [Abstract] [Full Text] [PDF]

				J. A. Harton and J. P.-Y. Ting Class II Transactivator: Mastering the Art of Major Histocompatibility Complex Expression Mol. Cell. Biol., September 1, 2000; 20(17): 6185 - 6194. [Full Text]