HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Currie, A. J. Articles by McWilliam, A. S. Search for Related Content PubMed PubMed Citation Articles by Currie, A. J. Articles by McWilliam, A. S.

Alveolar Macrophages Bind and Phagocytose Allergen- Containing Pollen Starch Granules Via C-Type Lectin and Integrin Receptors: Implications for Airway Inflammatory Disease¹

Andrew J. Currie^*,, Geoffrey A. Stewart^* and Andrew S. McWilliam^2,*,

^* Department of Microbiology, University of Western Australia, Nedlands, Western Australia; and Division of Cell Biology, TVW Telethon Institute for Child Health Research, West Perth, Western Australia

Recent studies suggest that IgE-independent mechanisms of airway inflammation contribute significantly to the pathophysiology of allergic airway inflammatory diseases such as asthma. Such mechanisms may involve direct interactions between inhaled allergens and cells of the respiratory tract such as macrophages, dendritic cells, and epithelial cells. In this study, we investigated receptor-mediated interactions occurring between alveolar macrophages and allergen-containing pollen starch granules (PSG). We report here that PSG are released from a range of grass species and are rapidly bound and phagocytosed by alveolar macrophages. Human monocyte-derived dendritic cells also bound PSG but no internalization was observed. Phagocytosis of PSG was dependent on Mg²⁺ and Ca²⁺ and was inhibited by neo-glycoproteins such as galactose-BSA and N-acetylgalactose-BSA. Partial inhibition of phagocytosis was also seen with the Arg-Gly-Asp-Ser (RGDS) motif and with an anti-CD18 mAb (OX42). The combination of both neo-glycoprotein and anti-CD18 achieved the greatest degree of inhibition (>90%). Together, these data suggest a role for both C-type lectins and ß₂-integrins in the binding and internalization of PSG. The consequences of this interaction included a rapid up-regulation of inducible NO synthase mRNA and subsequent release of NO by alveolar macrophages. Thus, receptor-mediated recognition of inhaled allergenic particles by alveolar macrophages may represent a potential mechanism for modulating the inflammatory response associated with allergic airway diseases such as asthma.

This article has been cited by other articles:

				J.-S. Shin, C. P. Shelburne, C. Jin, E. A. LeFurgey, and S. N. Abraham Harboring of Particulate Allergens within Secretory Compartments by Mast Cells following IgE/Fc{epsilon}RI-Lipid Raft-Mediated Phagocytosis J. Immunol., November 1, 2006; 177(9): 5791 - 5800. [Abstract] [Full Text] [PDF]

				D. C. Malherbe, V. J. Erpenbeck, S. N. Abraham, E. C. Crouch, J. M. Hohlfeld, and J. R. Wright Surfactant protein D decreases pollen-induced IgE-dependent mast cell degranulation Am J Physiol Lung Cell Mol Physiol, November 1, 2005; 289(5): L856 - L866. [Abstract] [Full Text] [PDF]

				E. Agea, A. Russano, O. Bistoni, R. Mannucci, I. Nicoletti, L. Corazzi, A. D. Postle, G. De Libero, S. A. Porcelli, and F. Spinozzi Human CD1-restricted T cell recognition of lipids from pollens J. Exp. Med., July 18, 2005; 202(2): 295 - 308. [Abstract] [Full Text] [PDF]

				V. J. Erpenbeck, D. C. Malherbe, S. Sommer, A. Schmiedl, W. Steinhilber, A. J. Ghio, N. Krug, J. R. Wright, and J. M. Hohlfeld Surfactant protein D increases phagocytosis and aggregation of pollen-allergen starch granules Am J Physiol Lung Cell Mol Physiol, April 1, 2005; 288(4): L692 - L698. [Abstract] [Full Text] [PDF]

				C.-L. Chen, C.-T. Lee, Y.-C. Liu, J.-Y. Wang, H.-Y. Lei, and C.-K. Yu House Dust Mite Dermatophagoides farinae Augments Proinflammatory Mediator Productions and Accessory Function of Alveolar Macrophages: Implications for Allergic Sensitization and Inflammation J. Immunol., January 1, 2003; 170(1): 528 - 536. [Abstract] [Full Text] [PDF]

				A. Kamachi, Y. Nasuhara, M. Nishimura, T. Takahashi, Y. Homma, Y. Ohtsuka, and M. Munakata Dissociation between airway responsiveness to methacholine and responsiveness to antigen Eur. Respir. J., January 1, 2002; 19(1): 76 - 83. [Abstract] [Full Text] [PDF]

				R. Fernandez, V. Sarma, E. Younkin, R. B. Hirschl, P. A. Ward, and J. G. Younger Exposure to perflubron is associated with decreased Syk phosphorylation in human neutrophils J Appl Physiol, November 1, 2001; 91(5): 1941 - 1947. [Abstract] [Full Text] [PDF]