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G551D Cystic Fibrosis Mice Exhibit Abnormal Regulation of Inflammation in Lungs and Macrophages¹

Gordon R. Thomas^*, Elaine A. Costelloe^*,, Dominic P. Lunn^*, Katryn J. Stacey^*,, Steven J. Delaney^*, Robert Passey^*,, Edwina C. McGlinn^*, Brendan J. McMorran^*, Azita Ahadizadeh^*, Carolyn L. Geczy, Brandon J. Wainwright^* and David A. Hume^2,*,

^* Centre for Molecular and Cellular Biology and Department of Biochemistry and Microbiology, University of Queensland, St. Lucia, Australia; and Cytokine Research Unit, School of Pathology, University of New South Wales, Kensington, Australia

The major cause of death in cystic fibrosis (CF) is chronic lung disease associated with persistent infection by the bacterium, Pseudomonas aeruginosa. S100A8, an S-100 calcium-binding protein with chemotactic activity, is constitutively expressed in the lungs and serum of CF patients. Levels of S100A8 mRNA were found to be three to four times higher in the lungs of mice carrying the G551D mutation in CF transmembrane conductance regulator compared with littermate controls. Intravenous injection of bacterial LPS induced S100A8 mRNA in the lung to a greater extent in G551D mice than in wild-type littermates. Localization of S100A8 mRNA and protein in the lung indicate that it is a marker for neutrophil accumulation. Bone marrow-derived macrophages from G551D mice were shown to also exhibit hypersensitivity to LPS, measured by induction of TNF-. These results provide evidence that the pathology of CF relates to abnormal regulation of the immune system.

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