| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Departments of
*
Anesthesiology,
Pathology,
Surgery, and
§
Medicine, Washington University School of Medicine, St. Louis, MO 63110
Sepsis induces extensive apoptosis of lymphocytes, which may be responsible for the profound immune suppression of the disorder. Two potential pathways of sepsis-induced lymphocyte apoptosis, Fas and p53, were investigated. Lymphocyte apoptosis was evaluated 20–22 h after sepsis by annexin V or DNA nick-end labeling. Fas receptor-deficient mice had no protection against sepsis-induced apoptosis in thymocytes or splenocytes. p53 knockout mice (p53-/-) had complete protection against thymocyte apoptosis but, surprisingly, had no protection in splenocytes. p53-/- mice had no improvement in sepsis survival compared with appropriately matched control mice with sepsis. We conclude that both p53-dependent and p53-independent pathways of cell death exist in sepsis. This differential apoptotic response of thymocytes vs splenocytes in p53-/- mice suggests that either the cellular response or the death-inducing signal is cell-type specific in sepsis. The fact that p53-/- lymphocytes of an identical subtype (CD8-CD4+) were protected in thymi but not in spleens indicates that cell susceptibility to apoptosis differs depending upon other unidentified factors.
This article has been cited by other articles:
|
|
 |
|
  O. M. Peck-Palmer, J. Unsinger, K. C. Chang, C. G. Davis, J. E. McDunn, and R. S. Hotchkiss Deletion of MyD88 markedly attenuates sepsis-induced T and B lymphocyte apoptosis but worsens survival J. Leukoc. Biol., April 1, 2008; 83(4): 1009 - 1018. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. C. Chang, J. Unsinger, C. G. Davis, S. J. Schwulst, J. T. Muenzer, A. Strasser, and R. S. Hotchkiss Multiple triggers of cell death in sepsis: death receptor and mitochondrial-mediated apoptosis FASEB J, March 1, 2007; 21(3): 708 - 719. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. O. Scumpia, M. J. Delano, K. M. Kelly, K. A. O'Malley, P. A. Efron, P. F. McAuliffe, T. Brusko, R. Ungaro, T. Barker, J. L. Wynn, et al. Increased Natural CD4+CD25+ Regulatory T Cells and Their Suppressor Activity Do Not Contribute to Mortality in Murine Polymicrobial Sepsis J. Immunol., December 1, 2006; 177(11): 7943 - 7949. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. J. Schwulst, M. H. Grayson, P. J. DiPasco, C. G. Davis, T. S. Brahmbhatt, T. A. Ferguson, and R. S. Hotchkiss Agonistic Monoclonal Antibody Against CD40 Receptor Decreases Lymphocyte Apoptosis and Improves Survival in Sepsis J. Immunol., July 1, 2006; 177(1): 557 - 565. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Suzuki, V. Kapoor, A. S. Jassar, L. R. Kaiser, and S. M. Albelda Gemcitabine Selectively Eliminates Splenic Gr-1+/CD11b+ Myeloid Suppressor Cells in Tumor-Bearing Animals and Enhances Antitumor Immune Activity Clin. Cancer Res., September 15, 2005; 11(18): 6713 - 6721. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. E. Wesche, J. L. Lomas-Neira, M. Perl, C.-S. Chung, and A. Ayala Leukocyte apoptosis and its significance in sepsis and shock J. Leukoc. Biol., August 1, 2005; 78(2): 325 - 337. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. S. Hotchkiss, S. B. Osmon, K. C. Chang, T. H. Wagner, C. M. Coopersmith, and I. E. Karl Accelerated Lymphocyte Death in Sepsis Occurs by both the Death Receptor and Mitochondrial Pathways J. Immunol., April 15, 2005; 174(8): 5110 - 5118. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. G. R. WEAVER, M. S. ROUSE, J. M. STECKELBERG, and A. D. BADLEY Improved survival in experimental sepsis with an orally administered inhibitor of apoptosis FASEB J, August 1, 2004; 18(11): 1185 - 1191. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
U. Bommhardt, K. C. Chang, P. E. Swanson, T. H. Wagner, K. W. Tinsley, I. E. Karl, and R. S. Hotchkiss Akt Decreases Lymphocyte Apoptosis and Improves Survival in Sepsis J. Immunol., June 15, 2004; 172(12): 7583 - 7591. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. W. Tinsley, M. H. Grayson, P. E. Swanson, A. M. Drewry, K. C. Chang, I. E. Karl, and R. S. Hotchkiss Sepsis Induces Apoptosis and Profound Depletion of Splenic Interdigitating and Follicular Dendritic Cells J. Immunol., July 15, 2003; 171(2): 909 - 914. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. ADRIE, M. BACHELET, M. VAYSSIER-TAUSSAT, F. RUSSO-MARIE, I. BOUCHAERT, M. ADIB-CONQUY, J.-M. CAVAILLON, M. R. PINSKY, J.-F. DHAINAUT, and B. S. POLLA Mitochondrial Membrane Potential and Apoptosis Peripheral Blood Monocytes in Severe Human Sepsis Am. J. Respir. Crit. Care Med., August 1, 2001; 164(3): 389 - 395. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. M. Grayson, J. G. Lanier, J. D. Altman, and R. Ahmed The Role of p53 in Regulating Antiviral T Cell Responses J. Immunol., August 1, 2001; 167(3): 1333 - 1337. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. S. Hotchkiss, K. W. Tinsley, P. E. Swanson, R. E. Schmieg Jr., J. J. Hui, K. C. Chang, D. F. Osborne, B. D. Freeman, J. P. Cobb, T. G. Buchman, et al. Sepsis-Induced Apoptosis Causes Progressive Profound Depletion of B and CD4+ T Lymphocytes in Humans J. Immunol., June 1, 2001; 166(11): 6952 - 6963. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C.-S. Chung, W. Wang, I. H. Chaudry, and A. Ayala Increased apoptosis in lamina propria B cells during polymicrobial sepsis is FasL but not endotoxin mediated Am J Physiol Gastrointest Liver Physiol, May 1, 2001; 280(5): G812 - G818. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
