HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Coyle, J. H. Articles by Lebman, D. A. Search for Related Content PubMed PubMed Citation Articles by Coyle, J. H. Articles by Lebman, D. A.

Correct Immunoglobulin mRNA Processing Depends on Specific Sequence in the C3-M Intron¹

Department of Microbiology and Immunology, Virginia Commonwealth University, Richmond, VA 23298.

The maturation of IgM-expressing B cells to IgM-secreting plasma cells is associated with both an increase in µ mRNA and the ratio of secreted to membrane forms of µ mRNA which differ at the 3' termini. In contrast, both in vitro and in vivo the secreted form of mRNA is predominant at all stages in the development of a secretory IgA response. Previous studies demonstrated that preferential usage of the s poly(A) site does not result from transcription termination and is independent of either the poly(A) sites or the 3' splice site associated with the exon encoding the membrane exon of IgA (M). The present study demonstrates that a 349-bp region located 774 bp 3' to the s poly(A) site is required for the preferential usage of the s terminus. This region, which is the first isotype-specific cis-acting regulatory sequence not immediately adjacent to a secretory poly(A) site to be identified, contains regulatory elements that increase the efficiency of polyadenylation/cleavage. A ubiquitous, 58-kDa RNA-binding protein interacts specifically with this regulatory region. These studies support the premise that cis-acting elements unique to each C_H gene can impinge upon a common mechanism regulating Ig mRNA processing.

This article has been cited by other articles:

				M. L. Peterson, S. Bertolino, and F. Davis An RNA Polymerase Pause Site Is Associated with the Immunoglobulin {micro}s Poly(A) Site Mol. Cell. Biol., August 1, 2002; 22(15): 5606 - 5615. [Abstract] [Full Text] [PDF]

				M. L. Lundqvist, D. L. Middleton, S. Hazard, and G. W. Warr The Immunoglobulin Heavy Chain Locus of the Duck. GENOMIC ORGANIZATION AND EXPRESSION OF D, J, AND C REGION GENES J. Biol. Chem., December 7, 2001; 276(50): 46729 - 46736. [Abstract] [Full Text] [PDF]