The JI Acurri Cytometers
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 QUICK SEARCH:   [advanced]


     
 


This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Oida, T.
Right arrow Articles by Ishikawa, H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Oida, T.
Right arrow Articles by Ishikawa, H.
The Journal of Immunology, 2000, 164: 3616-3626.
Copyright © 2000 by The American Association of Immunologists

Role of Gut Cryptopatches in Early Extrathymic Maturation of Intestinal Intraepithelial T Cells1

Takatoku Oida*,{dagger}, Kenji Suzuki*, Masanobu Nanno{ddagger}, Yutaka Kanamori§, Hisashi Saito, Eiro Kubota, Shingo Kato*, Mamoru Itoh||, Shuichi Kaminogawa{dagger} and Hiromichi Ishikawa2,*

* Department of Microbiology, Keio University School of Medicine, Tokyo, Japan; {dagger} Department of Applied Biological Chemistry, University of Tokyo, Tokyo, Japan; {ddagger} Yakult Central Institute for Microbiological Research, Tokyo, Japan; § Department of Pediatric Surgery, Faculty of Medicine, University of Tokyo, Tokyo, Japan; Second Department of Oral and Maxillofacial Surgery, Kanagawa Dental School, Kanagawa, Japan; and || Central Institute for Experimental Animals, Kanagawa, Japan

Lympho-hemopoietic progenitors residing in murine gut cryptopatches (CP) have been shown to generate intestinal intraepithelial T cells (IEL). To investigate the role of CP in progenitor maturation, we analyzed IEL in male mice with a truncated mutation of common cytokine receptor {gamma}-chain (CR{gamma}-/Y) in which CP were undetectable. IEL-expressing TCR-{gamma}{delta} ({gamma}{delta}-IEL) were absent, and a drastically reduced number of Thy-1highCD4+ and Thy-1highCD8{alpha}ß+ {alpha}ß-IEL were present in CR{gamma}-/Y mice, whereas these {alpha}ß-IEL disappeared from athymic CR{gamma}-/Y littermate mice. Athymic CR{gamma}-/Y mice possessed a small TCR- and {alpha}Eß7 integrin-negative IEL population, characterized by the disappearance of the extrathymic CD8{alpha}{alpha}+ subset, that expressed pre-T{alpha}, RAG-2, and TCR-Cß but not CD3{epsilon} transcripts. These TCR- IEL from athymic CR{gamma}-/Y mice did not undergo Dß-Jß and V{delta}-J{delta} joinings, despite normal rearrangements at the TCR-ß and -{delta} loci in thymocytes from euthymic CR{gamma}-/Y mice. In contrast, athymic severe combined immunodeficient mice in which CP developed normally possessed two major TCR-{alpha}Eß7+ CD8{alpha}{alpha}+ and CD8- IEL populations that expressed pre-T{alpha}, RAG-2, TCR-Cß, and CD3{epsilon} transcripts. These findings underscore the role of gut CP in the early extrathymic maturation of CD8{alpha}{alpha}+ IEL, including cell-surface expression of {alpha}Eß7 integrin, CD3{epsilon} gene transcription, and TCR gene rearrangements.







HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Website Copyright © 2000 by The American Association of Immunologists, Inc. All rights reserved.
All Contents Copyright © 2000 by The American Association of Immunologists, Inc. All rights reserved.