| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Department of Biomedical Engineering, University of Virginia Health Sciences Center, Charlottesville, VA 22908
Leukocyte rolling along the walls of inflamed venules precedes
their adhesion during inflammation. Rolling leukocytes are thought to
arrest by engaging ß2 integrins following cellular
activation. In vitro studies suggest that chemoattractants may
instantaneously activate and arrest rolling leukocytes. However, how
leukocytes stop rolling and become adherent in inflamed venules in vivo
has remained rather mysterious. In this paper we use a novel method of
tracking individual leukocytes through the microcirculation to show
that rolling neutrophils become progressively activated while rolling
down the venular tree. On average, leukocytes in wild-type mice roll
for 86 s (and cover 270 µm) before becoming adherent with an
efficiency around 90%. These rolling leukocytes exhibit a gradual
ß2 integrin-dependent decrease in rolling velocity that
correlates with an increase in intracellular free calcium concentration
before arrest. Similar tracking analyses in gene-targeted mice
demonstrate that the arrest of rolling leukocytes is very rare when
ß2 integrins are absent or blocked by a mAb. Arrest is
50% less efficient in the absence of E-selectin. These data suggest
a model of leukocyte recruitment in which ß2 integrins
play a critical role in stabilizing leukocyte rolling during a
protracted cellular activation period before arrest and firm
adhesion.
This article has been cited by other articles:
|
|
 |
|
  A. Zarbock, H. Muller, Y. Kuwano, and K. Ley PSGL-1-dependent myeloid leukocyte activation J. Leukoc. Biol., November 1, 2009; 86(5): 1119 - 1124. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Petri, M. Phillipson, and P. Kubes The Physiology of Leukocyte Recruitment: An In Vivo Perspective J. Immunol., May 15, 2008; 180(10): 6439 - 6446. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. L. Nolan, N. Kalia, G. B. Nash, D. Kamel, P. Heeringa, and C. O.S. Savage Mechanisms of ANCA-Mediated Leukocyte-Endothelial Cell Interactions In Vivo J. Am. Soc. Nephrol., May 1, 2008; 19(5): 973 - 984. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Zarbock, T. L. Deem, T. L. Burcin, and K. Ley G{alpha}i2 is required for chemokine-induced neutrophil arrest Blood, November 15, 2007; 110(10): 3773 - 3779. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Sarraj, K. Ludanyi, T. T. Glant, A. Finnegan, and K. Mikecz Expression of CD44 and L-Selectin in the Innate Immune System Is Required for Severe Joint Inflammation in the Proteoglycan-Induced Murine Model of Rheumatoid Arthritis J. Immunol., August 1, 2006; 177(3): 1932 - 1940. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. Carriere, R. Colisson, C. Jiguet-Jiglaire, E. Bellard, G. Bouche, T. Al Saati, F. Amalric, J.-P. Girard, and C. M'Rini Cancer Cells Regulate Lymphocyte Recruitment and Leukocyte-Endothelium Interactions in the Tumor-Draining Lymph Node Cancer Res., December 15, 2005; 65(24): 11639 - 11648. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. C. Schafer, D. N. Sehrt, M. Kamler, H. Jakob, and H.-A. Lehr Paradoxical attenuation of leukocyte rolling in response to ischemia- reperfusion and extracorporeal blood circulation in inflamed tissue Am J Physiol Heart Circ Physiol, July 1, 2005; 289(1): H330 - H335. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. W. Saeed, S. Varma, T. Peng-Nemeroff, B. Sherry, D. Balakhaneh, J. Huston, K. J. Tracey, Y. Al-Abed, and C. N. Metz Cholinergic stimulation blocks endothelial cell activation and leukocyte recruitment during inflammation J. Exp. Med., April 4, 2005; 201(7): 1113 - 1123. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. P. Burns and N. DePaola Flow-conditioned HUVECs support clustered leukocyte adhesion by coexpressing ICAM-1 and E-selectin Am J Physiol Heart Circ Physiol, January 1, 2005; 288(1): H194 - H204. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. H. Embury, N. M. Matsui, S. Ramanujam, T. N. Mayadas, C. T. Noguchi, B. A. Diwan, N. Mohandas, and A. T. W. Cheung The contribution of endothelial cell P-selectin to the microvascular flow of mouse sickle erythrocytes in vivo Blood, November 15, 2004; 104(10): 3378 - 3385. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. L. Smith, T. S. Olson, and K. Ley CXCR2- and E-Selectin-induced Neutrophil Arrest during Inflammation In Vivo J. Exp. Med., October 4, 2004; 200(7): 935 - 939. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. Ramachandran, M. Williams, T. Yago, D. W. Schmidtke, and R. P. McEver Dynamic alterations of membrane tethers stabilize leukocyte rolling on P-selectin PNAS, September 14, 2004; 101(37): 13519 - 13524. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Xu, A. Manivannan, H.-R. Jiang, J. Liversidge, P. F. Sharp, J. V. Forrester, and I. J. Crane Recruitment of IFN-{gamma}-Producing (Th1-Like) Cells into the Inflamed Retina In Vivo Is Preferentially Regulated by P-Selectin Glycoprotein Ligand 1:P/E-Selectin Interactions J. Immunol., March 1, 2004; 172(5): 3215 - 3224. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Zhang, A. Chen, D. De Leon, H. Li, E. Noiri, V. T. Moy, and M. S. Goligorsky Atomic force microscopy measurement of leukocyte-endothelial interaction Am J Physiol Heart Circ Physiol, January 1, 2004; 286(1): H359 - H367. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. L. Dunne, R. G. Collins, A. L. Beaudet, C. M. Ballantyne, and K. Ley Mac-1, but Not LFA-1, Uses Intercellular Adhesion Molecule-1 to Mediate Slow Leukocyte Rolling in TNF-{alpha}-Induced Inflammation J. Immunol., December 1, 2003; 171(11): 6105 - 6111. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Ley Sulfated Sugars for Rolling Lymphocytes J. Exp. Med., November 3, 2003; 198(9): 1285 - 1288. [Full Text] [PDF]
|
|
|
|
|
|
M. Sperandio, M. L. Smith, S. B. Forlow, T. S. Olson, L. Xia, R. P. McEver, and K. Ley P-selectin Glycoprotein Ligand-1 Mediates L-Selectin-dependent Leukocyte Rolling in Venules J. Exp. Med., May 19, 2003; 197(10): 1355 - 1363. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. E. R. Hicks, S. L. Nolan, V. C. Ridger, P. G. Hellewell, and K. E. Norman Recombinant P-selectin glycoprotein ligand-1 directly inhibits leukocyte rolling by all 3 selectins in vivo: complete inhibition of rolling is not required for anti-inflammatory effect Blood, April 15, 2003; 101(8): 3249 - 3256. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. AMON, M. D. MENGER, and B. VOLLMAR Heme oxygenase and nitric oxide synthase mediate cooling-associated protection against TNF-{alpha}-induced microcirculatory dysfunction and apoptotic cell death FASEB J, February 1, 2003; 17(2): 175 - 185. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. J. Prorock, A. Hafezi-Moghadam, V. E. Laubach, J. K. Liao, and K. Ley Vascular protection by estrogen in ischemia-reperfusion injury requires endothelial nitric oxide synthase Am J Physiol Heart Circ Physiol, January 1, 2003; 284(1): H133 - H140. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Kadono, G. M. Venturi, D. A. Steeber, and T. F. Tedder Leukocyte Rolling Velocities and Migration Are Optimized by Cooperative L-Selectin and Intercellular Adhesion Molecule-1 Functions J. Immunol., October 15, 2002; 169(8): 4542 - 4550. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. B. FORLOW, P. L. FOLEY, and K. LEY Severely reduced neutrophil adhesion and impaired host defense against fecal and commensal bacteria in CD18-/-P-selectin-/- double null mice FASEB J, October 1, 2002; 16(12): 1488 - 1496. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. S. Olson and K. Ley Chemokines and chemokine receptors in leukocyte trafficking Am J Physiol Regulatory Integrative Comp Physiol, July 1, 2002; 283(1): R7 - R28. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. F. H. Lum, C. E. Green, G. R. Lee, D. E. Staunton, and S. I. Simon Dynamic Regulation of LFA-1 Activation and Neutrophil Arrest on Intercellular Adhesion Molecule 1 (ICAM-1) in Shear Flow J. Biol. Chem., May 31, 2002; 277(23): 20660 - 20670. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. L. Dunne, C. M. Ballantyne, A. L. Beaudet, and K. Ley Control of leukocyte rolling velocity in TNF-alpha -induced inflammation by LFA-1 and Mac-1 Blood, January 1, 2002; 99(1): 336 - 341. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. A. DiVietro, M. J. Smith, B. R. E. Smith, L. Petruzzelli, R. S. Larson, and M. B. Lawrence Immobilized IL-8 Triggers Progressive Activation of Neutrophils Rolling In Vitro on P-Selectin and Intercellular Adhesion Molecule-1 J. Immunol., October 1, 2001; 167(7): 4017 - 4025. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. J. Cotter, K. E. Norman, P. G. Hellewell, and V. C. Ridger A Novel Method for Isolation of Neutrophils from Murine Blood Using Negative Immunomagnetic Separation Am. J. Pathol., August 1, 2001; 159(2): 473 - 481. [Abstract] [Full Text]
|
|
|
|
|
|
R. B. Henderson, L. H.K. Lim, P. A. Tessier, F. N.E. Gavins, M. Mathies, M. Perretti, and N. Hogg The Use of Lymphocyte Function-Associated Antigen (Lfa)-1-Deficient Mice to Determine the Role of Lfa-1, Mac-1, and {alpha}4 Integrin in the Inflammatory Response of Neutrophils J. Exp. Med., July 16, 2001; 194(2): 219 - 226. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Singbartl, J. Thatte, M. L. Smith, K. Wethmar, K. Day, and K. Ley A CD2-Green Fluorescence Protein-Transgenic Mouse Reveals Very Late Antigen-4-Dependent CD8+ Lymphocyte Rolling in Inflamed Venules J. Immunol., June 15, 2001; 166(12): 7520 - 7526. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. E. ERIKSSON, X. XIE, J. WERR, P. THOREN, and L. LINDBOM Direct viewing of atherosclerosis in vivo: plaque invasion by leukocytes is initiated by the endothelial selectins FASEB J, May 1, 2001; 15(7): 1149 - 1157. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. C. Ridger, B. E. Wagner, W. A. H. Wallace, and P. G. Hellewell Differential Effects of CD18, CD29, and CD49 Integrin Subunit Inhibition on Neutrophil Migration in Pulmonary Inflammation J. Immunol., March 1, 2001; 166(5): 3484 - 3490. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. M. Mariscalco, W. Vergara, J. Mei, E. O'B. Smith, and C. W. Smith Mechanisms of decreased leukocyte localization in the developing host Am J Physiol Heart Circ Physiol, February 1, 2002; 282(2): H636 - H644. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
