| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Immune Cell Interaction Unit, Mucosal Immunity Section, Laboratory for Clinical Investigation, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892
We investigated the ability of chemoattractants to affect IL-12
production by human monocytes and dendritic cells. We found that
pretreatment of monocytes with macrophage chemoattractant proteins
(MCP-1 to -4), or C5a, but not stromal-derived factor-1, macrophage
inflammatory protein-1
, RANTES, or eotaxin, inhibited IL-12 p70
production in response to stimulation with Staphylococcus
aureus, Cowan strain 1 (SAC), and IFN-
. The production of
TNF- and IL-10, however, was minimally affected by any of the
chemoattractants. The degree of inhibition of IL-12 p70 production by
MCP-1 to -4 was donor dependent and was affected by the autocrine
inhibitory effects of IL-10. In contrast, C5a profoundly suppressed
IL-12 production in an IL-10-independent fashion. Neither TGF-ß1 nor
PGE2 was important for the suppression of IL-12 by any of
the chemoattractants tested. The accumulation of mRNA for both IL-12
p35 and p40 genes was inhibited by chemokine pretreatment.
Interestingly, MCP-1 to -4 and C5a did not suppress IL-12 production by
monocyte-derived dendritic cells (DC) stimulated with CD40 ligand and
IFN- or by SAC and IFN-, suggesting that these factors may act at
the site of inflammation to suppress IL-12 and IFN- production
rather than in the lymph node to affect T cell priming. Despite the
inability of C5a to inhibit IL-12 production by DCs, the receptor for
C5a (CD88) was expressed by these cells, and recombinant C5a induced a
Ca2+ flux. Taken together, these results define a range of
chemoattractant molecules with the ability to suppress IL-12 production
by human monocytes and have broad implications for the regulation of
immune responses in vivo.
This article has been cited by other articles:
|
|
 |
|
  M. Del Corno, A. Michienzi, A. Masotti, L. Da Sacco, G. F. Bottazzo, F. Belardelli, and S. Gessani CC chemokine ligand 2 down-modulation by selected Toll-like receptor agonist combinations contributes to T helper 1 polarization in human dendritic cells Blood, July 23, 2009; 114(4): 796 - 806. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. N. Lalli, M. G. Strainic, F. Lin, M. E. Medof, and P. S. Heeger Decay Accelerating Factor Can Control T Cell Differentiation into IFN-{gamma}-Producing Effector Cells via Regulating Local C5a-Induced IL-12 Production J. Immunol., November 1, 2007; 179(9): 5793 - 5802. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. A. Moulton, M. A. Mashruwala, A. K. Smith, D. R. Lindsey, R. A. Wetsel, D. L. Haviland, R. L. Hunter, and C. Jagannath Complement C5a anaphylatoxin is an innate determinant of dendritic cell-induced Th1 immunity to Mycobacterium bovis BCG infection in mice J. Leukoc. Biol., October 1, 2007; 82(4): 956 - 967. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. A. Byrnes, D.-Y. Li, K. Park, D. Thompson, C. Mocilnikar, P. Mohan, J. P. Molleston, M. Narkewicz, H. Zhou, S. F. Wolf, et al. Modulation of the IL-12/IFN-{gamma} axis by IFN-{alpha} therapy for hepatitis C J. Leukoc. Biol., March 1, 2007; 81(3): 825 - 834. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. A. Melendi, S. J. Hoffman, R. A. Karron, P. M. Irusta, F. R. Laham, A. Humbles, B. Schofield, C.-H. Pan, R. Rabold, B. Thumar, et al. C5 Modulates Airway Hyperreactivity and Pulmonary Eosinophilia during Enhanced Respiratory Syncytial Virus Disease by Decreasing C3a Receptor Expression J. Virol., January 15, 2007; 81(2): 991 - 999. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. O. Flores-Villanueva, J. A. Ruiz-Morales, C.-H. Song, L. M. Flores, E.-K. Jo, M. Montano, P. F. Barnes, M. Selman, and J. Granados A functional promoter polymorphism in monocyte chemoattractant protein-1 is associated with increased susceptibility to pulmonary tuberculosis J. Exp. Med., December 19, 2005; 202(12): 1649 - 1658. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. E. Wenderfer, B. Ke, T. J. Hollmann, R. A. Wetsel, H. Y. Lan, and M. C. Braun C5a Receptor Deficiency Attenuates T Cell Function and Renal Disease in MRLlpr Mice J. Am. Soc. Nephrol., December 1, 2005; 16(12): 3572 - 3582. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Appel, V. Mirakaj, A. Bringmann, M. M. Weck, F. Grunebach, and P. Brossart PPAR-{gamma} agonists inhibit toll-like receptor-mediated activation of dendritic cells via the MAP kinase and NF-{kappa}B pathways Blood, December 1, 2005; 106(12): 3888 - 3894. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. W. DePaolo, R. Lathan, B. J. Rollins, and W. J. Karpus The Chemokine CCL2 Is Required for Control of Murine Gastric Salmonella enterica Infection Infect. Immun., October 1, 2005; 73(10): 6514 - 6522. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. N. Waggoner, M. W. Cruise, R. Kassel, and Y. S. Hahn gC1q Receptor Ligation Selectively Down-Regulates Human IL-12 Production through Activation of the Phosphoinositide 3-Kinase Pathway J. Immunol., October 1, 2005; 175(7): 4706 - 4714. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. la Sala, M. Gadina, and B. L. Kelsall Gi-Protein-Dependent Inhibition of IL-12 Production Is Mediated by Activation of the Phosphatidylinositol 3-Kinase-Protein 3 Kinase B/Akt Pathway and JNK J. Immunol., September 1, 2005; 175(5): 2994 - 2999. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Elhofy, J. Wang, M. Tani, B. T. Fife, K. J. Kennedy, J. Bennett, D. Huang, R. M. Ransohoff, and W. J. Karpus Transgenic expression of CCL2 in the central nervous system prevents experimental autoimmune encephalomyelitis J. Leukoc. Biol., February 1, 2005; 77(2): 229 - 237. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Antoniazi, H. P. Price, P. Kropf, M. A. Freudenberg, C. Galanos, D. F. Smith, and I. Muller Chemokine Gene Expression in Toll-Like Receptor-Competent and -Deficient Mice Infected with Leishmania major Infect. Immun., September 1, 2004; 72(9): 5168 - 5174. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. H. J. Kim, I. D. Dimitriou, M. C. H. Holland, D. Mastellos, Y. M. Mueller, J. D. Altman, J. D. Lambris, and P. D. Katsikis Complement C5a Receptor Is Essential for the Optimal Generation of Antiviral CD8+ T Cell Responses J. Immunol., August 15, 2004; 173(4): 2524 - 2529. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Zhou, H. Xiong, H. Li, S. E. Plevy, P. D. Walden, M. Sassaroli, G. D. Prestwich, and J. C. Unkeless Microtubule-Associated Serine/Threonine Kinase-205 kDa and Fc{gamma} Receptor Control IL-12 p40 Synthesis and NF-{kappa}B Activation J. Immunol., February 15, 2004; 172(4): 2559 - 2568. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. S. Klein, L. Izikson, T. Means, H. D. Gibson, E. Lin, R. A. Sobel, H. L. Weiner, and A. D. Luster IFN-Inducible Protein 10/CXC Chemokine Ligand 10-Independent Induction of Experimental Autoimmune Encephalomyelitis J. Immunol., January 1, 2004; 172(1): 550 - 559. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Taube, Y.-H. Rha, K. Takeda, J.-W. Park, A. Joetham, A. Balhorn, A. Dakhama, P. C. Giclas, V. M. Holers, and E. W. Gelfand Inhibition of Complement Activation Decreases Airway Inflammation and Hyperresponsiveness Am. J. Respir. Crit. Care Med., December 1, 2003; 168(11): 1333 - 1341. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. L. deSchoolmeester, M. C. Little, B. J. Rollins, and K. J. Else Absence of CC Chemokine Ligand 2 Results in an Altered Th1/Th2 Cytokine Balance and Failure to Expel Trichuris muris Infection J. Immunol., May 1, 2003; 170(9): 4693 - 4700. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Soruri, Z. Kiafard, C. Dettmer, J. Riggert, J. Kohl, and J. Zwirner IL-4 Down-Regulates Anaphylatoxin Receptors in Monocytes and Dendritic Cells and Impairs Anaphylatoxin-Induced Migration In Vivo J. Immunol., March 15, 2003; 170(6): 3306 - 3314. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Zaph and P. Scott Interleukin-12 Regulates Chemokine Gene Expression during the Early Immune Response to Leishmania major Infect. Immun., March 1, 2003; 71(3): 1587 - 1589. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Dalwadi, B. Wei, M. Schrage, T. T. Su, D. J. Rawlings, and J. Braun B Cell Developmental Requirement for the G{alpha}i2 Gene J. Immunol., February 15, 2003; 170(4): 1707 - 1715. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. M. Drouin, D. B. Corry, T. J. Hollman, J. Kildsgaard, and R. A. Wetsel Absence of the Complement Anaphylatoxin C3a Receptor Suppresses Th2 Effector Functions in a Murine Model of Pulmonary Allergy J. Immunol., November 15, 2002; 169(10): 5926 - 5933. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Omata, M. Yasutomi, A. Yamada, H. Iwasaki, M. Mayumi, and Y. Ohshima Monocyte Chemoattractant Protein-1 Selectively Inhibits the Acquisition of CD40 Ligand-Dependent IL-12-Producing Capacity of Monocyte-Derived Dendritic Cells and Modulates Th1 Immune Response J. Immunol., November 1, 2002; 169(9): 4861 - 4866. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Locati, U. Deuschle, M. L. Massardi, F. O. Martinez, M. Sironi, S. Sozzani, T. Bartfai, and A. Mantovani Analysis of the Gene Expression Profile Activated by the CC Chemokine Ligand 5/RANTES and by Lipopolysaccharide in Human Monocytes J. Immunol., April 1, 2002; 168(7): 3557 - 3562. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. F. Lipscomb and B. J. Masten Dendritic Cells: Immune Regulators in Health and Disease Physiol Rev, January 1, 2002; 82(1): 97 - 130. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. KRUG, T. TSCHERNIG, V. J. ERPENBECK, J. M. HOHLFELD, and J. KOHL Complement Factors C3a and C5a Are Increased in Bronchoalveolar Lavage Fluid after Segmental Allergen Provocation in Subjects with Asthma Am. J. Respir. Crit. Care Med., November 15, 2001; 164(10): 1841 - 1843. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Q. Huang, D. Liu, P. Majewski, L. C. Schulte, J. M. Korn, R. A. Young, E. S. Lander, and N. Hacohen The Plasticity of Dendritic Cell Responses to Pathogens and Their Components Science, October 26, 2001; 294(5543): 870 - 875. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. M. Drouin, D. B. Corry, J. Kildsgaard, and R. A. Wetsel Cutting Edge: The Absence of C3 Demonstrates a Role for Complement in Th2 Effector Functions in a Murine Model of Pulmonary Allergy J. Immunol., October 15, 2001; 167(8): 4141 - 4145. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. S. Gerber and D. M. Mosser Reversing Lipopolysaccharide Toxicity by Ligating the Macrophage Fc{{gamma}} Receptors J. Immunol., June 1, 2001; 166(11): 6861 - 6868. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. C. Braun, J. M. Wang, E. Lahey, R. L. Rabin, and B. L. Kelsall Activation of the formyl peptide receptor by the HIV-derived peptide T-20 suppresses interleukin-12 p70 production by human monocytes Blood, June 1, 2001; 97(11): 3531 - 3536. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R.-F. Guo, A. B. Lentsch, R. L. Warner, M. Huber-Lang, J. V. Sarma, T. Hlaing, M. M. Shi, N. W. Lukacs, and P. A. Ward Regulatory Effects of Eotaxin on Acute Lung Inflammatory Injury J. Immunol., April 15, 2001; 166(8): 5208 - 5218. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Corinti, C. Albanesi, A. la Sala, S. Pastore, and G. Girolomoni Regulatory Activity of Autocrine IL-10 on Dendritic Cell Functions J. Immunol., April 1, 2001; 166(7): 4312 - 4318. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Matsunaga, T. W. Klein, C. Newton, H. Friedman, and Y. Yamamoto Legionella pneumophila Suppresses Interleukin-12 Production by Macrophages Infect. Immun., March 1, 2001; 69(3): 1929 - 1933. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Nakachi, K. Matsunaga, T. W. Klein, H. Friedman, and Y. Yamamoto Differential Effects of Virulent versus Avirulent Legionella pneumophila on Chemokine Gene Expression in Murine Alveolar Macrophages Determined by cDNA Expression Array Technique Infect. Immun., October 1, 2000; 68(10): 6069 - 6072. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. He, S. Gurunathan, A. Iwasaki, B. Ash-Shaheed, and B. L. Kelsall Primary Role for GI Protein Signaling in the Regulation of Interleukin 12 Production and the Induction of T Helper Cell Type 1 Responses J. Exp. Med., May 1, 2000; 191(9): 1605 - 1610. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
