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The Journal of Immunology, 2000, 164: 2971-2977.
Copyright © 2000 by The American Association of Immunologists

Development and Analysis of Various Clonal Alloantigen- Dependent Cytotoxic Cell Lines from Channel Catfish1 ,2

Tor B. Stuge, Melanie R. Wilson, He Zhou, Katherine S. Barker, Eva Bengtén, Greg Chinchar, Norman W. Miller and L. William Clem3

Department of Microbiology, University of Mississippi Medical Center, Jackson, MS 39216

To determine the phenotypes of cytotoxic cells in channel catfish, clonal alloantigen-dependent leukocyte lines were established from mixed leukocyte cultures. Each clone was analyzed for expression of TCR {alpha} and ß genes by RT-PCR and for target cell specificity by 51Cr-release assay. Based on the above criteria, the following five different cell types were identified among the 19 clones analyzed: 1) TCR {alpha}ß+ allospecific cytotoxic cells, 2) TCR {alpha}ß+ nonspecific cytotoxic cells, 3) allospecific TCR {alpha}ß+ noncytotoxic cells, 4) TCR {alpha}ß- nonspecific cytotoxic cells, and 5) TCR {alpha}ß- allospecific cytotoxic cells. The demonstration of cloned, TCR {alpha}ß+, allospecific cytotoxic effectors provides the strongest evidence to date for the existence of cytotoxic T cells in fish.




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