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Department of Microbiology, University of Mississippi Medical Center, Jackson, MS 39216
To determine the phenotypes of cytotoxic cells in channel
catfish, clonal alloantigen-dependent leukocyte lines were established
from mixed leukocyte cultures. Each clone was analyzed for expression
of TCR 
and ß genes by RT-PCR and for target cell specificity by
51Cr-release assay. Based on the above criteria, the
following five different cell types were identified among the 19 clones
analyzed: 1) TCR ß+ allospecific cytotoxic cells, 2)
TCR ß+ nonspecific cytotoxic cells, 3) allospecific
TCR ß+ noncytotoxic cells, 4) TCR ß-
nonspecific cytotoxic cells, and 5) TCR ß-
allospecific cytotoxic cells. The demonstration of cloned, TCR
ß+, allospecific cytotoxic effectors provides the
strongest evidence to date for the existence of cytotoxic T cells in
fish.
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