|
|
||||||||


*
Department of Medical Microbiology and Immunology, University of Aarhus, Aarhus, Denmark;
Medical Research Council Immunochemistry Unit, Department of Biochemistry, University of Oxford, Oxford, United Kingdom; and
Immunology and Microbiology, Institute of Medical Biology, University of Southern Denmark, Odense, Denmark
Mannan-binding lectin (MBL) is a serum protein that
activates the complement system after binding to glycoconjugates found
on the surface of microorganisms. By molecular cloning two forms of MBL
have been identified in the mouse (mMBL-A and mMBL-C), but only mMBL-A
has been purified and characterized at the protein level. MBL-C has
been termed the liver form of MBL. The present report describes the
purification and characterization of mMBL-A and mMBL-C from serum. The
two forms of mMBL could be separated both by ion-exchange and
carbohydrate-affinity chromatography. The initial identification by
immunochemical technique was confirmed by N-terminal amino-acid
sequencing. Both proteins give bands corresponding to polypeptide
chains of 28 kDa on SDS-PAGE in the reduced state, but mMBL-A migrated
more rapidly than mMBL-C in acid/urea-PAGE, in accordance with the
calculated pIs. Both forms mediated activation of complement component
C4 in mannan-coated microtiter wells. MBL-A showed a higher affinity
for D-glucose and
-methyl-D-glucose then did
MBL-C. Serum concentrations of mMBL-A in laboratory strains and wild
mice were found to vary from 5 to 80 µg/ml, with wild mice tending to
show higher levels than laboratory strains.
This article has been cited by other articles:
![]() |
D.-M. Zuo, L.-Y. Zhang, X. Lu, Y. Liu, and Z.-L. Chen Protective role of mouse MBL-C on intestinal mucosa during Shigella flexneri invasion Int. Immunol., October 1, 2009; 21(10): 1125 - 1134. [Abstract] [Full Text] [PDF] |
||||
![]() |
K. L. Mershon, A. Vasuthasawat, G. W. Lawson, S. L. Morrison, and D. O. Beenhouwer Role of Complement in Protection against Cryptococcus gattii Infection Infect. Immun., March 1, 2009; 77(3): 1061 - 1070. [Abstract] [Full Text] [PDF] |
||||
![]() |
N. K. Banda, K. Takahashi, A. K. Wood, V. M. Holers, and W. P. Arend Pathogenic Complement Activation in Collagen Antibody- Induced Arthritis in Mice Requires Amplification by the Alternative Pathway J. Immunol., September 15, 2007; 179(6): 4101 - 4109. [Abstract] [Full Text] [PDF] |
||||
![]() |
T. Carter, M. Sumiya, K. Reilly, R. Ahmed, P. Sobieszczuk, J. A. Summerfield, and R. A. Lawrence Mannose-Binding Lectin A-Deficient Mice Have Abrogated Antigen-Specific IgM Responses and Increased Susceptibility to a Nematode Infection J. Immunol., April 15, 2007; 178(8): 5116 - 5123. [Abstract] [Full Text] [PDF] |
||||
![]() |
E. C. Crouch, K. Smith, B. McDonald, D. Briner, B. Linders, J. McDonald, U. Holmskov, J. Head, and K. Hartshorn Species Differences in the Carbohydrate Binding Preferences of Surfactant Protein D Am. J. Respir. Cell Mol. Biol., July 1, 2006; 35(1): 84 - 94. [Abstract] [Full Text] [PDF] |
||||
![]() |
K. Takahashi, L. Shi, L. D. Gowda, and R. A. B. Ezekowitz Relative Roles of Complement Factor 3 and Mannose-Binding Lectin in Host Defense against Infection Infect. Immun., December 1, 2005; 73(12): 8188 - 8193. [Abstract] [Full Text] [PDF] |
||||
![]() |
J. Seyfarth, P. Garred, and H. O. Madsen The 'involution' of mannose-binding lectin Hum. Mol. Genet., October 1, 2005; 14(19): 2859 - 2869. [Abstract] [Full Text] [PDF] |
||||
![]() |
M. Hirano, B. Y. Ma, N. Kawasaki, K. Okimura, M. Baba, T. Nakagawa, K. Miwa, N. Kawasaki, S. Oka, and T. Kawasaki Mannan-Binding Protein Blocks the Activation of Metalloproteases Meprin {alpha} and {beta} J. Immunol., September 1, 2005; 175(5): 3177 - 3185. [Abstract] [Full Text] [PDF] |
||||
![]() |
M. Windbichler, B. Echtenacher, T. Hehlgans, J. C. Jensenius, W. Schwaeble, and D. N. Mannel Involvement of the Lectin Pathway of Complement Activation in Antimicrobial Immune Defense during Experimental Septic Peritonitis Infect. Immun., September 1, 2004; 72(9): 5247 - 5252. [Abstract] [Full Text] [PDF] |
||||
![]() |
B. T. MacDonald, M. Adamska, and M. H. Meisler Hypomorphic expression of Dkk1 in the doubleridge mouse: dose dependence and compensatory interactions with Lrp6 Development, June 1, 2004; 131(11): 2543 - 2552. [Abstract] [Full Text] [PDF] |
||||
![]() |
L. Shi, K. Takahashi, J. Dundee, S. Shahroor-Karni, S. Thiel, J. C. Jensenius, F. Gad, M. R. Hamblin, K. N. Sastry, and R. A. B. Ezekowitz Mannose-binding Lectin-deficient Mice Are Susceptible to Infection with Staphylococcus aureus J. Exp. Med., May 17, 2004; 199(10): 1379 - 1390. [Abstract] [Full Text] [PDF] |
||||
![]() |
F. Larsen, H. O. Madsen, R. B. Sim, C. Koch, and P. Garred Disease-associated Mutations in Human Mannose-binding Lectin Compromise Oligomerization and Activity of the Final Protein J. Biol. Chem., May 14, 2004; 279(20): 21302 - 21311. [Abstract] [Full Text] [PDF] |
||||
![]() |
C. M. Hogaboam, K. Takahashi, R. A. B. Ezekowitz, S. L. Kunkel, and J. M. Schuh Mannose-binding lectin deficiency alters the development of fungal asthma: effects on airway response, inflammation, and cytokine profile J. Leukoc. Biol., May 1, 2004; 75(5): 805 - 814. [Abstract] [Full Text] [PDF] |
||||
![]() |
S. Wagner, N. J. Lynch, W. Walter, W. J. Schwaeble, and M. Loos Differential Expression of the Murine Mannose-Binding Lectins A and C in Lymphoid and Nonlymphoid Organs and Tissues J. Immunol., February 1, 2003; 170(3): 1462 - 1465. [Abstract] [Full Text] [PDF] |
||||
![]() |
K. Uemura, M. Saka, T. Nakagawa, N. Kawasaki, S. Thiel, J. C. Jensenius, and T. Kawasaki L-MBP Is Expressed in Epithelial Cells of Mouse Small Intestine J. Immunol., December 15, 2002; 169(12): 6945 - 6950. [Abstract] [Full Text] [PDF] |
||||
![]() |
S. J. Lee, G. Gonzalez-Aseguinolaza, and M. C. Nussenzweig Disseminated Candidiasis and Hepatic Malarial Infection in Mannose-Binding-Lectin-A-Deficient Mice Mol. Cell. Biol., December 1, 2002; 22(23): 8199 - 8203. [Abstract] [Full Text] [PDF] |
||||
![]() |
S. Hansen, D. Holm, V. Moeller, L. Vitved, C. Bendixen, K. B. M. Reid, K. Skjoedt, and U. Holmskov CL-46, a Novel Collectin Highly Expressed in Bovine Thymus and Liver J. Immunol., November 15, 2002; 169(10): 5726 - 5734. [Abstract] [Full Text] [PDF] |
||||
![]() |
T. A. Baldwin and H. L. Ostergaard Developmentally Regulated Changes in Glucosidase II Association with, and Carbohydrate Content of, the Protein Tyrosine Phosphatase CD45 J. Immunol., October 1, 2001; 167(7): 3829 - 3835. [Abstract] [Full Text] [PDF] |
||||
![]() |
J. E. Jordan, M. C. Montalto, and G. L. Stahl Inhibition of Mannose-Binding Lectin Reduces Postischemic Myocardial Reperfusion Injury Circulation, September 18, 2001; 104(12): 1413 - 1418. [Abstract] [Full Text] [PDF] |
||||
![]() |
P. Opanasopit, K. Shirashi, M. Nishikawa, F. Yamashita, Y. Takakura, and M. Hashida In vivo recognition of mannosylated proteins by hepatic mannose receptors and mannan-binding protein Am J Physiol Gastrointest Liver Physiol, May 1, 2001; 280(5): G879 - G889. [Abstract] [Full Text] [PDF] |
||||
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |