| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
*
Department of Pathology, Massachusetts General Hospital, and
Physiology Program, Harvard School of Public Health, Boston, MA 02129
Bone marrow-derived dendritic cell (DC) precursors migrate via the blood stream to peripheral tissues to adopt their sentinel function. To identify factors facilitating their emigration to the lung, mutant mice deficient in E-selectin, P-selectin, E/P-selectin, ICAM-1, or CD18 and their respective controls were examined. DCs and monocytes/macrophages were immunolabeled with M5/114 and MOMA-2 mAbs, respectively, and quantified morphometrically. Of these genotypes, the numbers of DC and MOMA-2+ cells were significantly less only in the lungs of CD18-/- mice by 68 and 35% in alveolar walls and by 28 and 26% in venous walls, respectively. DCs were reduced by 30 and 41% around large and small airways, respectively, but the number of MOMA-2+ cells in these locations was not significantly different from controls. Ablation of a single gene may be associated with augmented expression of other, related gene products. Therefore, we examined the expression of VCAM-1. Increased numbers of arteries exhibited continuous luminal VCAM-1 staining in both CD18-/- and ICAM-1-/- mutants. VCAM-1 expression was absent in pulmonary capillaries and unchanged in veins. These data suggest that under nonperturbing conditions, CD18-mediated adhesion is required for the full complement of DC precursors to accumulate in the lungs. However, the defect in CD18-/- mice is partial, suggesting that CD18-independent adhesion occurs. The alternative pathway may involve VLA-4/VCAM-1 in arteries and venules but not in capillaries. The smaller defect in ICAM-1-/- mice suggests that the CD11/CD18 complex recognizes ligands other than ICAM-1 at some sites.
This article has been cited by other articles:
|
|
 |
|
  J. McGill, N. Van Rooijen, and K. L. Legge Protective influenza-specific CD8 T cell responses require interactions with dendritic cells in the lungs J. Exp. Med., July 7, 2008; 205(7): 1635 - 1646. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Ichiyasu, J. M. McCormack, K. M. McCarthy, D. Dombkowski, F. I. Preffer, and E. E. Schneeberger Matrix Metalloproteinase-9-Deficient Dendritic Cells Have Impaired Migration through Tracheal Epithelial Tight Junctions Am. J. Respir. Cell Mol. Biol., June 1, 2004; 30(6): 761 - 770. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. T. Pribila, A. A. Itano, K. L. Mueller, and Y. Shimizu The {alpha}1{beta}1 and {alpha}E{beta}7 Integrins Define a Subset of Dendritic Cells in Peripheral Lymph Nodes with Unique Adhesive and Antigen Uptake Properties J. Immunol., January 1, 2004; 172(1): 282 - 291. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Y. Vermaelen, D. Cataldo, K. Tournoy, T. Maes, A. Dhulst, R. Louis, J.-M. Foidart, A. Noel, and R. Pauwels Matrix Metalloproteinase-9-Mediated Dendritic Cell Recruitment into the Airways Is a Critical Step in a Mouse Model of Asthma J. Immunol., July 15, 2003; 171(2): 1016 - 1022. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Sakurai, H. Taguchi, A. Anand, B. K. Ambati, E. S. Gragoudas, J. W. Miller, A. P. Adamis, and J. Ambati Targeted Disruption of the CD18 or ICAM-1 Gene Inhibits Choroidal Neovascularization Invest. Ophthalmol. Vis. Sci., June 1, 2003; 44(6): 2743 - 2749. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Fiorini, W. Vermi, F. Facchetti, D. Moratto, G. Alessandri, L. Notarangelo, A. Caruso, P. Grigolato, A. G. Ugazio, L. D. Notarangelo, et al. Defective migration of monocyte-derived dendritic cells in LAD-1 immunodeficiency J. Leukoc. Biol., October 1, 2002; 72(4): 650 - 656. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Gironella, M. Molla, A. Salas, A. Soriano, M. Sans, D. Closa, P. Engel, A. Salas, J. M. Pique, and J. Panes The role of P-selectin in experimental colitis as determined by antibody immunoblockade and genetically deficient mice J. Leukoc. Biol., July 1, 2002; 72(1): 56 - 64. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 U. MAUS, J. HUWE, L. ERMERT, M. ERMERT, W. SEEGER, and J. LOHMEYER Molecular Pathways of Monocyte Emigration into the Alveolar Air Space of Intact Mice Am. J. Respir. Crit. Care Med., January 1, 2002; 165(1): 95 - 100. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Bouvard, C. Brakebusch, E. Gustafsson, A. Aszodi, T. Bengtsson, A. Berna, and R. Fassler Functional Consequences of Integrin Gene Mutations in Mice Circ. Res., July 30, 2001; 89(3): 211 - 223. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
