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Stat6 Regulation of In Vivo IL-4 Responses¹

Fred D. Finkelman^2,*, Suzanne C. Morris^*, Tatyana Orekhova^*, Masaaki Mori^3,*, Debra Donaldson, Steven L. Reiner, Nancy L. Reilly, Lisa Schopf^§ and Joseph F. Urban, Jr.^§

^* Division of Immunology, University of Cincinnati College of Medicine, Cincinnati, OH 45267, and Cincinnati Veterans Administration Medical Center, Cincinnati, OH 45220; Genetics Institute, Cambridge, MA 02140; University of Pennsylvania School of Medicine, Philadelphia, PA 19104; and ^§ U.S. Department of Agriculture, Beltsville, MD 20705

Although in vitro development of a Th2 response from naive CD4⁺ T cells is Stat6 dependent, mice immunized with a goat Ab to mouse IgD have been reported to produce a normal primary IL-4 response in Stat6-deficient mice. Experiments have now been performed with mice immunized with more conventional Ags or inoculated with nematode parasites to account for this apparent discrepancy. The ability of an immunogen to induce a primary in vivo IL-4 response in Stat6-deficient mice was found to vary directly with its ability to induce a strong type 2 cytokine-biased response in normal mice. Even immunogens, however, that induce strong primary IL-4 responses in Stat6-deficient mice induce poor memory IL-4 responses in these mice. Consistent with this, Stat6-deficient CD4⁺ T cells make relatively normal IL-4 responses when stimulated in vitro for 3 days with anti-CD3 and anti-CD28, but poor IL-4 responses if they are later restimulated with anti-CD3. Thus, Stat6 signaling enhances primary IL-4 responses that are made as part of a type 0 cytokine response (mixed type 1 and type 2) and is required for normal development or survival of Th2 memory cells.