HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Matsushita, M. Articles by Fujita, T. Search for Related Content PubMed PubMed Citation Articles by Matsushita, M. Articles by Fujita, T. Pubmed/NCBI databases Gene GEO Profiles HomoloGene Protein UniGene Substance via MeSH

Cutting Edge: Complement-Activating Complex of Ficolin and Mannose-Binding Lectin-Associated Serine Protease¹

Department of Biochemistry, Fukushima Medical University School of Medicine, Fukushima, Japan

Both ficolins and mannose-binding lectin (MBL) are lectins characterized by the presence of collagen-like and carbohydrate-binding domains in a subunit, although their carbohydrate-binding moieties are quite different. A fibrinogen-like domain is in ficolins, and a carbohydrate recognition domain is in MBL. On binding to pathogens, human MBL activates the complement system via the lectin pathway in association with two types of MBL-associated serine proteases (MASP), MASP-1 and MASP-2 and its truncated form, small MBL-associated protein (sMAP, also called MAp19). We report here that ficolin/P35, a human serum ficolin, was found to copurify with MASPs and sMAP. MASPs that were complexed with ficolin/P35 exhibited proteolytic activities against complement components C4, C2, and C3. The ficolin/P35-MASPs-sMAP complex that was bound to Salmonella typhimurium activated complement. These findings indicate that ficolin/P35 is a second collagenous lectin capable of activating the lectin pathway and thus plays a role in innate immunity.

This article has been cited by other articles:

				M. Lacroix, C. Dumestre-Perard, G. Schoehn, G. Houen, J.-Y. Cesbron, G. J. Arlaud, and N. M. Thielens Residue Lys57 in the Collagen-Like Region of Human L-Ficolin and Its Counterpart Lys47 in H-Ficolin Play a Key Role in the Interaction with the Mannan-Binding Lectin-Associated Serine Proteases and the Collectin Receptor Calreticulin J. Immunol., January 1, 2009; 182(1): 456 - 465. [Abstract] [Full Text] [PDF]

				F. Teillet, C. Gaboriaud, M. Lacroix, L. Martin, G. J. Arlaud, and N. M. Thielens Crystal Structure of the CUB1-EGF-CUB2 Domain of Human MASP-1/3 and Identification of Its Interaction Sites with Mannan-binding Lectin and Ficolins J. Biol. Chem., September 12, 2008; 283(37): 25715 - 25724. [Abstract] [Full Text] [PDF]

				D. Liu, J.-Y. Zhu, and Z.-X. Niu Molecular Structure and Expression of Anthropic, Ovine, and Murine Forms of Complement Receptor Type 2 Clin. Vaccine Immunol., June 1, 2008; 15(6): 901 - 910. [Full Text] [PDF]

				E. Shagdarsuren, K. Bidzhekov, Y. Djalali-Talab, E. A. Liehn, M. Hristov, R. A. Matthijsen, W. A. Buurman, A. Zernecke, and C. Weber C1-Esterase Inhibitor Protects Against Neointima Formation After Arterial Injury in Atherosclerosis-Prone Mice Circulation, January 1, 2008; 117(1): 70 - 78. [Abstract] [Full Text] [PDF]

				A. Roos, M. R. Daha, J. van Pelt, and S. P. Berger Mannose-binding lectin and the kidney Nephrol. Dial. Transplant., December 1, 2007; 22(12): 3370 - 3377. [Full Text] [PDF]

				S. Mihai, M. T. Chiriac, K. Takahashi, J. M. Thurman, V. M. Holers, D. Zillikens, M. Botto, and C. Sitaru The Alternative Pathway of Complement Activation Is Critical for Blister Induction in Experimental Epidermolysis Bullosa Acquisita J. Immunol., May 15, 2007; 178(10): 6514 - 6521. [Abstract] [Full Text] [PDF]

				R. Wallis, A. W. Dodds, D. A. Mitchell, R. B. Sim, K. B. M. Reid, and W. J. Schwaeble Molecular Interactions between MASP-2, C4, and C2 and Their Activation Fragments Leading to Complement Activation via the Lectin Pathway J. Biol. Chem., March 16, 2007; 282(11): 7844 - 7851. [Abstract] [Full Text] [PDF]

				M. Tanio, S. Kondo, S. Sugio, and T. Kohno Trivalent Recognition Unit of Innate Immunity System: CRYSTAL STRUCTURE OF TRIMERIC HUMAN M-FICOLIN FIBRINOGEN-LIKE DOMAIN J. Biol. Chem., February 9, 2007; 282(6): 3889 - 3895. [Abstract] [Full Text] [PDF]

				A. Roos, M. P. Rastaldi, N. Calvaresi, B. D. Oortwijn, N. Schlagwein, D. J. van Gijlswijk-Janssen, G. L. Stahl, M. Matsushita, T. Fujita, C. van Kooten, et al. Glomerular Activation of the Lectin Pathway of Complement in IgA Nephropathy Is Associated with More Severe Renal Disease J. Am. Soc. Nephrol., June 1, 2006; 17(6): 1724 - 1734. [Abstract] [Full Text] [PDF]

				J. M. Thurman and V. M. Holers The Central Role of the Alternative Complement Pathway in Human Disease J. Immunol., February 1, 2006; 176(3): 1305 - 1310. [Abstract] [Full Text] [PDF]

				V. Rossi, F. Teillet, N. M. Thielens, I. Bally, and G. J. Arlaud Functional Characterization of Complement Proteases C1s/Mannan-binding Lectin-associated Serine Protease-2 (MASP-2) Chimeras Reveals the Higher C4 Recognition Efficacy of the MASP-2 Complement Control Protein Modules J. Biol. Chem., December 23, 2005; 280(51): 41811 - 41818. [Abstract] [Full Text] [PDF]

				Y. Liu, Y. Endo, D. Iwaki, M. Nakata, M. Matsushita, I. Wada, K. Inoue, M. Munakata, and T. Fujita Human M-Ficolin Is a Secretory Protein That Activates the Lectin Complement Pathway J. Immunol., September 1, 2005; 175(5): 3150 - 3156. [Abstract] [Full Text] [PDF]

				T. Hummelshoj, L. Munthe-Fog, H. O. Madsen, T. Fujita, M. Matsushita, and P. Garred Polymorphisms in the FCN2 gene determine serum variation and function of Ficolin-2 Hum. Mol. Genet., June 15, 2005; 14(12): 1651 - 1658. [Abstract] [Full Text] [PDF]

				N. J. Lynch, S.-u.-H. Khan, C. M. Stover, S. M. Sandrini, D. Marston, J. S. Presanis, and W. J. Schwaeble Composition of the Lectin Pathway of Complement in Gallus gallus: Absence of Mannan-Binding Lectin-Associated Serine Protease-1 in Birds J. Immunol., April 15, 2005; 174(8): 4998 - 5006. [Abstract] [Full Text] [PDF]

				A. Krarup, U. B. S. Sorensen, M. Matsushita, J. C. Jensenius, and S. Thiel Effect of Capsulation of Opportunistic Pathogenic Bacteria on Binding of the Pattern Recognition Molecules Mannan-Binding Lectin, L-Ficolin, and H-Ficolin Infect. Immun., February 1, 2005; 73(2): 1052 - 1060. [Abstract] [Full Text] [PDF]

				Y. Aoyagi, E. E. Adderson, J. G. Min, M. Matsushita, T. Fujita, S. Takahashi, Y. Okuwaki, and J. F. Bohnsack Role of L-Ficolin/Mannose-Binding Lectin-Associated Serine Protease Complexes in the Opsonophagocytosis of Type III Group B Streptococci J. Immunol., January 1, 2005; 174(1): 418 - 425. [Abstract] [Full Text] [PDF]

				A. Krarup, S. Thiel, A. Hansen, T. Fujita, and J. C. Jensenius L-ficolin Is a Pattern Recognition Molecule Specific for Acetyl Groups J. Biol. Chem., November 12, 2004; 279(46): 47513 - 47519. [Abstract] [Full Text] [PDF]

				M. Windbichler, B. Echtenacher, T. Hehlgans, J. C. Jensenius, W. Schwaeble, and D. N. Mannel Involvement of the Lectin Pathway of Complement Activation in Antimicrobial Immune Defense during Experimental Septic Peritonitis Infect. Immun., September 1, 2004; 72(9): 5247 - 5252. [Abstract] [Full Text] [PDF]

				L. A. Gregory, N. M. Thielens, M. Matsushita, R. Sorensen, G. J. Arlaud, J. C. Fontecilla-Camps, and C. Gaboriaud The X-ray Structure of Human Mannan-binding Lectin-associated Protein 19 (MAp19) and Its Interaction Site with Mannan-binding Lectin and L-ficolin J. Biol. Chem., July 9, 2004; 279(28): 29391 - 29397. [Abstract] [Full Text] [PDF]

				M. Matsushita, A. Matsushita, Y. Endo, M. Nakata, N. Kojima, T. Mizuochi, and T. Fujita Origin of the classical complement pathway: Lamprey orthologue of mammalian C1q acts as a lectin PNAS, July 6, 2004; 101(27): 10127 - 10131. [Abstract] [Full Text] [PDF]

				Y. G. Ma, M. Y. Cho, M. Zhao, J. W. Park, M. Matsushita, T. Fujita, and B. L. Lee Human Mannose-binding Lectin and L-Ficolin Function as Specific Pattern Recognition Proteins in the Lectin Activation Pathway of Complement J. Biol. Chem., June 11, 2004; 279(24): 25307 - 25312. [Abstract] [Full Text] [PDF]

				R. Wallis, J. M. Shaw, J. Uitdehaag, C.-B. Chen, D. Torgersen, and K. Drickamer Localization of the Serine Protease-binding Sites in the Collagen-like Domain of Mannose-binding Protein: INDIRECT EFFECTS OF NATURALLY OCCURRING MUTATIONS ON PROTEASE BINDING AND ACTIVATION J. Biol. Chem., April 2, 2004; 279(14): 14065 - 14073. [Abstract] [Full Text] [PDF]

				S. Zundel, S. Cseh, M. Lacroix, M. R. Dahl, M. Matsushita, J.-P. Andrieu, W. J. Schwaeble, J. C. Jensenius, T. Fujita, G. J. Arlaud, et al. Characterization of Recombinant Mannan-Binding Lectin-Associated Serine Protease (MASP)-3 Suggests an Activation Mechanism Different from That of MASP-1 and MASP-2 J. Immunol., April 1, 2004; 172(7): 4342 - 4350. [Abstract] [Full Text] [PDF]

				T. Ohashi and H. P. Erickson The Disulfide Bonding Pattern in Ficolin Multimers J. Biol. Chem., February 20, 2004; 279(8): 6534 - 6539. [Abstract] [Full Text] [PDF]

				N. J. Lynch, S. Roscher, T. Hartung, S. Morath, M. Matsushita, D. N. Maennel, M. Kuraya, T. Fujita, and W. J. Schwaeble L-Ficolin Specifically Binds to Lipoteichoic Acid, a Cell Wall Constituent of Gram-Positive Bacteria, and Activates the Lectin Pathway of Complement J. Immunol., January 15, 2004; 172(2): 1198 - 1202. [Abstract] [Full Text] [PDF]

				Y. Endo, M. Nonaka, H. Saiga, Y. Kakinuma, A. Matsushita, M. Takahashi, M. Matsushita, and T. Fujita Origin of Mannose-Binding Lectin-Associated Serine Protease (MASP)-1 and MASP-3 Involved in the Lectin Complement Pathway Traced Back to the Invertebrate, Amphioxus J. Immunol., May 1, 2003; 170(9): 4701 - 4707. [Abstract] [Full Text] [PDF]

				M. S. Quesenberry, H. Ahmed, M. T. Elola, N. O'Leary, and G. R. Vasta Diverse Lectin Repertoires in Tunicates Mediate Broad Recognition and Effector Innate Immune Responses Integr. Comp. Biol., April 1, 2003; 43(2): 323 - 330. [Abstract] [Full Text] [PDF]

				M. Kuraya, M. Matsushita, Y. Endo, S. Thiel, and T. Fujita Expression of H-ficolin/Hakata antigen, mannose-binding lectin-associated serine protease (MASP)-1 and MASP-3 by human glioma cell line T98G Int. Immunol., January 1, 2003; 15(1): 109 - 117. [Abstract] [Full Text] [PDF]

				S. Cseh, L. Vera, M. Matsushita, T. Fujita, G. J. Arlaud, and N. M. Thielens Characterization of the Interaction Between L-Ficolin/P35 and Mannan-Binding Lectin-Associated Serine Proteases-1 and -2 J. Immunol., November 15, 2002; 169(10): 5735 - 5743. [Abstract] [Full Text] [PDF]

				O. Neth, D. L. Jack, M. Johnson, N. J. Klein, and M. W. Turner Enhancement of Complement Activation and Opsonophagocytosis by Complexes of Mannose-Binding Lectin with Mannose-Binding Lectin-Associated Serine Protease After Binding to Staphylococcus aureus J. Immunol., October 15, 2002; 169(8): 4430 - 4436. [Abstract] [Full Text] [PDF]

				Y. Endo, M. Takahashi, M. Kuraya, M. Matsushita, C. M. Stover, W. J. Schwaeble, and T. Fujita Functional characterization of human mannose-binding lectin-associated serine protease (MASP)-1/3 and MASP-2 promoters, and comparison with the C1s promoter Int. Immunol., October 1, 2002; 14(10): 1193 - 1201. [Abstract] [Full Text] [PDF]

				G. Dimopoulos, G. K. Christophides, S. Meister, J. Schultz, K. P. White, C. Barillas-Mury, and F. C. Kafatos Genome expression analysis of Anopheles gambiae: Responses to injury, bacterial challenge, and malaria infection PNAS, June 25, 2002; 99(13): 8814 - 8819. [Abstract] [Full Text] [PDF]

				S. Gulati, K. Sastry, J. C. Jensenius, P. A. Rice, and S. Ram Regulation of the Mannan-Binding Lectin Pathway of Complement on Neisseria gonorrhoeae by C1-Inhibitor and {alpha}2-Macroglobulin J. Immunol., April 15, 2002; 168(8): 4078 - 4086. [Abstract] [Full Text] [PDF]

				M. Matsushita, M. Kuraya, N. Hamasaki, M. Tsujimura, H. Shiraki, and T. Fujita Activation of the Lectin Complement Pathway by H-Ficolin (Hakata Antigen) J. Immunol., April 1, 2002; 168(7): 3502 - 3506. [Abstract] [Full Text] [PDF]

				I. Celik, C. Stover, M. Botto, S. Thiel, S. Tzima, D. Kunkel, M. Walport, W. Lorenz, and W. Schwaeble Role of the Classical Pathway of Complement Activation in Experimentally Induced Polymicrobial Peritonitis Infect. Immun., December 1, 2001; 69(12): 7304 - 7309. [Abstract] [Full Text] [PDF]

				H. Sekine, A. Kenjo, K. Azumi, G. Ohi, M. Takahashi, R. Kasukawa, N. Ichikawa, M. Nakata, T. Mizuochi, M. Matsushita, et al. An Ancient Lectin-Dependent Complement System in an Ascidian: Novel Lectin Isolated from the Plasma of the Solitary Ascidian, Halocynthia roretzi J. Immunol., October 15, 2001; 167(8): 4504 - 4510. [Abstract] [Full Text] [PDF]

				M. J. Walport Complement- Second of Two Parts N. Engl. J. Med., April 12, 2001; 344(15): 1140 - 1144. [Full Text] [PDF]

				M. Matsushita, S. Thiel, J. C. Jensenius, I. Terai, and T. Fujita Proteolytic Activities of Two Types of Mannose-Binding Lectin-Associated Serine Protease J. Immunol., September 1, 2000; 165(5): 2637 - 2642. [Abstract] [Full Text] [PDF]

				S. Thiel, S. V. Petersen, T. Vorup-Jensen, M. Matsushita, T. Fujita, C. M. Stover, W. J. Schwaeble, and J. C. Jensenius Interaction of C1q and Mannan-Binding Lectin (MBL) with C1r, C1s, MBL-Associated Serine Proteases 1 and 2, and the MBL-Associated Protein MAp19 J. Immunol., July 15, 2000; 165(2): 878 - 887. [Abstract] [Full Text] [PDF]

				A. Kenjo, M. Takahashi, M. Matsushita, Y. Endo, M. Nakata, T. Mizuochi, and T. Fujita Cloning and Characterization of Novel Ficolins from the Solitary Ascidian, Halocynthia roretzi J. Biol. Chem., June 1, 2001; 276(23): 19959 - 19965. [Abstract] [Full Text] [PDF]

				S. Tsuji, J. Uehori, M. Matsumoto, Y. Suzuki, A. Matsuhisa, K. Toyoshima, and T. Seya Human Intelectin Is a Novel Soluble Lectin That Recognizes Galactofuranose in Carbohydrate Chains of Bacterial Cell Wall J. Biol. Chem., June 22, 2001; 276(26): 23456 - 23463. [Abstract] [Full Text] [PDF]