The JI
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 QUICK SEARCH:   [advanced]


     
 


This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Request Permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Samandari, T.
Right arrow Articles by Sztein, M. B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Samandari, T.
Right arrow Articles by Sztein, M. B.
The Journal of Immunology, 2000, 164: 2221-2232.
Copyright © 2000 by The American Association of Immunologists

Production of IFN-{gamma} and IL-10 to Shigella Invasins by Mononuclear Cells from Volunteers Orally Inoculated with a Shiga Toxin-Deleted Shigella dysenteriae Type 1 Strain1

Taraz Samandari*, Karen L. Kotloff*, Genevieve A. Losonsky*, William D. Picking{dagger}, Philippe J. Sansonetti{ddagger}, Myron M. Levine* and Marcelo B. Sztein2,*

* Center for Vaccine Development, Departments of Pediatrics and Medicine, University of Maryland School of Medicine, Baltimore, MD 21201; {dagger} Department of Biology, St. Louis University, St. Louis, MO 63103; and {ddagger} Unité de Pathogénie Microbienne Moléculaire, Unité 389, Institut Nationale de la Santé et de la Recherche Médicale, Institut Pasteur, Paris, France

Volunteers were orally administered invasive, non-Shiga toxin-producing Shigella dysenteriae 1 to establish a challenge model to assess vaccine efficacy. In stepwise fashion, four separate groups were given 3 x 102, 7 x 103, 5 x 104, or 7 x 105 CFU. Using PBMC, proliferative responses and cytokine production were measured to S. dysenteriae whole-cell preparations and to purified recombinant invasion plasmid Ags (Ipa) C and IpaD. Anti-LPS and anti-Ipa Abs and Ab-secreting cells were also evaluated. Preinoculation PBMC produced considerable quantities of IL-10 and IFN-{gamma}, probably secreted by monocytes and NK cells, respectively, of the innate immune system. Following inoculation, PBMC from 95 and 87% of volunteers exhibited an increased production of IFN-{gamma} and IL-10, respectively, in response to Shigella Ags. These increases included responses to IpaC and IpaD among those volunteers receiving the lowest inoculum. No IL-4 or IL-5 responses were detected. Whereas there were no Ab or Ab-secreting cell responses in volunteers receiving the lowest inoculum, other dose groups had moderate to strong anti-LPS and anti-Ipa responses. These results suggest that in humans, type 1 responses play an important role in mucosal and systemic immunity to S. dysentariae 1.




This article has been cited by other articles:


Home page
Infect. Immun.Home page
K. L. Kotloff, D. N. Taylor, M. B. Sztein, S. S. Wasserman, G. A. Losonsky, J. P. Nataro, M. Venkatesan, A. Hartman, W. D. Picking, D. E. Katz, et al.
Phase I Evaluation of {Delta}virG Shigella sonnei Live, Attenuated, Oral Vaccine Strain WRSS1 in Healthy Adults
Infect. Immun., April 1, 2002; 70(4): 2016 - 2021.
[Abstract] [Full Text] [PDF]


Home page
Infect. Immun.Home page
M. L. Bernardini, J. Arondel, I. Martini, A. Aidara, and P. J. Sansonetti
Parameters Underlying Successful Protection with Live Attenuated Mutants in Experimental Shigellosis
Infect. Immun., February 1, 2001; 69(2): 1072 - 1083.
[Abstract] [Full Text] [PDF]


Home page
Infect. Immun.Home page
K. R. Turbyfill, A. B. Hartman, and E. V. Oaks
Isolation and Characterization of a Shigella flexneri Invasin Complex Subunit Vaccine
Infect. Immun., December 1, 2000; 68(12): 6624 - 6632.
[Abstract] [Full Text] [PDF]




HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Website Copyright © 2000 by The American Association of Immunologists, Inc. All rights reserved.
All Contents Copyright © 2000 by The American Association of Immunologists, Inc. All rights reserved.