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The Journal of Immunology, 2000, 164: 1862-1872.
Copyright © 2000 by The American Association of Immunologists

Single-Cytokine-Producing CD4 Memory Cells Predominate in Type 1 and Type 2 Immunity1

Alexey Y. Karulin, Maike D. Hesse, Magdalena Tary-Lehmann and Paul V. Lehmann2

Department of Pathology, Case Western Reserve University, Cleveland, OH 44106

The patterns of Ag-induced cytokine coexpression in normal, in vivo-primed CD4 memory T cells has remained controversial because the low frequency at which these cells occur has effectively prevented direct ex vivo measurements. We have overcome this limitation by using two-color cytokine enzyme-linked immunospot assays and computer-assisted image analysis. We found CD4 memory cells that simultaneously expressed IL-2, IL-3, IL-4, IL-5, and IFN-{gamma} to be rare (0–10%). This cytokine segregation was seen in adjuvant-induced type 1, type 2, and mixed immunity to OVA, in Leishmania infection regardless of the Ag dose used or how long after immunization the assay was performed. The data suggest that type 1 and type 2 immunity in vivo is not mediated by classic Th1 or Th2 cells but by single-cytokine-producing memory cells.







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