| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
*
Department of Clinical Chemistry, Microbiology and Immunology, University of Ghent, University Hospital of Ghent, Ghent, Belgium; and
Ludwig Institute for Cancer Research, Brussels Branch and Experimental Medicine Unit, Catholic University of Louvain, Brussels, Belgium
Highly purified human CD34+ hemopoietic precursor cells
differentiate into mature T cells when seeded in vitro in isolated
fetal thymic lobes of SCID mice followed by fetal thymus organ culture
(FTOC). Here, this chimeric human-mouse FTOC was used to address the
role of IL-9 and of the 
-chain of the IL-9 receptor (IL-9R) in
early human T cell development. We report that addition of the mAb
AH9R7, which recognizes and blocks selectively the human high affinity
-chain of the IL-9R, results in a profound reduction of the number
of human thymocytes. Analysis of lymphoid subpopulations indicates that
a highly reduced number of cells undergo maturation from
CD34+ precursor cells toward
CD4+CD3-CD8-CD1+
progenitor cells and subsequently toward
CD4+CD8+ double positive (DP) thymocytes.
Addition of IL-9 to the FTOC resulted in an increase in cell number,
without disturbing the frequencies of the different subsets. These data
suggest that IL-9R signaling is critical in early T lymphoid
development.
This article has been cited by other articles:
|
|
 |
|
  J. BURKHARDT, E. PETIT-TEIXEIRA, V. H. TEIXEIRA, H. KIRSTEN, S. GARNIER, S. RUEHLE, C. OESER, G. WOLFRAM, M. SCHOLZ, P. MIGLIORINI, et al. Association of the X-Chromosomal Genes TIMP1 and IL9R with Rheumatoid Arthritis J Rheumatol, October 1, 2009; 36(10): 2149 - 2157. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Q. Jiang, J. Huang, W. Q. Li, T. Cavinato, J. R. Keller, and S. K. Durum Role of the Intracellular Domain of IL-7 Receptor in T Cell Development J. Immunol., January 1, 2007; 178(1): 228 - 234. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Qiu, R. Lai, Q. Lin, E. Lau, D. M. Thomazy, D. Calame, R. J. Ford, L. W. Kwak, R. A. Kirken, and H. M. Amin Autocrine release of interleukin-9 promotes Jak3-dependent survival of ALK+ anaplastic large-cell lymphoma cells Blood, October 1, 2006; 108(7): 2407 - 2415. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E Melen, H Gullsten, M Zucchelli, A Lindstedt, F Nyberg, M Wickman, G Pershagen, and J Kere Sex specific protective effects of interleukin-9 receptor haplotypes on childhood wheezing and sensitisation J. Med. Genet., December 1, 2004; 41(12): e123 - e123. [Full Text] [PDF]
|
|
|
|
|
|
C. Gutierrez-Frias, R. Sacedon, C. Hernandez-Lopez, T. Cejalvo, T. Crompton, A. G. Zapata, A. Varas, and A. Vicente Sonic Hedgehog Regulates Early Human Thymocyte Differentiation by Counteracting the IL-7-Induced Development of CD34+ Precursor Cells J. Immunol., October 15, 2004; 173(8): 5046 - 5053. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Utsuyama, J. Shiraishi, H. Takahashi, M. Kasai, and K. Hirokawa Glia maturation factor produced by thymic epithelial cells plays a role in T cell differentiation in the thymic microenvironment Int. Immunol., May 1, 2003; 15(5): 557 - 564. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Stassen, C. Muller, M. Arnold, L. Hultner, S. Klein-Hessling, C. Neudorfl, T. Reineke, E. Serfling, and E. Schmitt IL-9 and IL-13 Production by Activated Mast Cells Is Strongly Enhanced in the Presence of Lipopolysaccharide: NF-{{kappa}}B Is Decisively Involved in the Expression of IL-9 J. Immunol., April 1, 2001; 166(7): 4391 - 4398. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
