| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
,
*
Department of Biochemistry, McGill University, Montreal, Canada;
Department of Biochemistry and Pediatrics, University of Ottawa, Ottawa, Canada; and
Apoptogen, Inc., Ottawa, Ontario, Canada
Legionella pneumophila is an intracellular pathogen that causes Legionnaires’ disease in humans. Inbred mouse strains are uniformly resistant to L. pneumophila infection with the notable exception of A/J, where the chromosome 13 locus Lgn1 renders A/J macrophages permissive to L. pneumophila replication. The mouse Lgn1 region is syntenic with the spinal muscular atrophy (SMA) locus on human chromosome 5 and includes several copies of the neuronal apoptosis inhibitory protein (Naip) gene. We have analyzed a possible link among Lgn1, Naip, and macrophage function. RNA expression studies show that Naip (mostly copy 2) mRNA transcripts are expressed in macrophage-rich tissues, such as spleen, lung, and liver and are abundant in primary macrophages. Immunoblotting and immunoprecipitation analyses identify Naip protein expression in mouse macrophages and in macrophage cell lines RAW 264.7 and J774A. Interestingly, macrophages from permissive A/J mice express significantly less Naip protein than their nonpermissive C57BL/6J counterpart. Naip protein expression is increased after phagocytic events. Naip protein levels during infection with either virulent or avirulent strains of L. pneumophila increase during the first 6 h postinfection and remain elevated during the 48-h observation period. This enhanced expression is also observed in macrophages infected with Salmonella typhimurium. Likewise, an increase in Naip protein levels in macrophages is observed 24 h after phagocytosis of Latex beads. The cosegregation of Lgn1 and Naip together with the detected Naip protein expression in host macrophages as well as its modulation after phagocytic events and during intracellular infection make it an attractive candidate for the Lgn1 locus.
This article has been cited by other articles:
|
|
 |
|
  A. Hebb, C. Moore, V Bhan, T Campbell, J. Fisk, H. Robertson, M Thorne, E Lacasse, M Holcik, J Gillard, et al. Expression of the inhibitor of apoptosis protein family in multiple sclerosis reveals a potential immunomodulatory role during autoimmune mediated demyelination Multiple Sclerosis, June 1, 2008; 14(5): 577 - 594. [Abstract] [PDF]
|
|
|
|
 |
|
 J. K.X. Maier, S. Balabanian, C. R. Coffill, A. Stewart, L. Pelletier, D. J. Franks, N. H. Gendron, and A. E. MacKenzie Distribution of Neuronal Apoptosis Inhibitory Protein in Human Tissues J. Histochem. Cytochem., September 1, 2007; 55(9): 911 - 923. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Asare, M. Santic, I. Gobin, M. Doric, J. Suttles, J. E. Graham, C. D. Price, and Y. Abu Kwaik Genetic Susceptibility and Caspase Activation in Mouse and Human Macrophages Are Distinct for Legionella longbeachae and L. pneumophila Infect. Immun., April 1, 2007; 75(4): 1933 - 1945. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. A. Archer and C. R. Roy MyD88-Dependent Responses Involving Toll-Like Receptor 2 Are Important for Protection and Clearance of Legionella pneumophila in a Mouse Model of Legionnaires' Disease. Infect. Immun., June 1, 2006; 74(6): 3325 - 3333. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Fortier, G. Min-Oo, J. Forbes, S. Lam-Yuk-Tseung, and P. Gros Single gene effects in mouse models of host: pathogen interactions J. Leukoc. Biol., June 1, 2005; 77(6): 868 - 877. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Watarai, I. Derre, J. Kirby, J. D. Growney, W. F. Dietrich, and R. R. Isberg Legionella pneumophila Is Internalized by a Macropinocytotic Uptake Pathway Controlled by the Dot/Icm System and the Mouse Lgn1 Locus J. Exp. Med., October 15, 2001; 194(8): 1081 - 1096. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. F. Dietrich Using Mouse Genetics to Understand Infectious Disease Pathogenesis Genome Res., March 1, 2001; 11(3): 325 - 331. [Full Text]
|
|
|
|
 |
|
 L. Aravind, V. M. Dixit, and E. V. Koonin Apoptotic Molecular Machinery: Vastly Increased Complexity in Vertebrates Revealed by Genome Comparisons Science, February 16, 2001; 291(5507): 1279 - 1284. [Abstract] [Full Text]
|
|
|
|
|
|
M. G. Endrizzi, V. Hadinoto, J. D. Growney, W. Miller, and W. F. Dietrich Genomic Sequence Analysis of the Mouse Naip Gene Array Genome Res., August 1, 2000; 10(8): 1095 - 1102. [Abstract] [Full Text]
|
|
|
|
|
|
J. D. Growney and W. F. Dietrich High-resolution Genetic and Physical Map of the Lgn1 Interval in C57BL/6J Implicates Naip2 or Naip5 in Legionella pneumophila Pathogenesis Genome Res., August 1, 2000; 10(8): 1158 - 1171. [Abstract] [Full Text]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
