| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
-Chain
Laboratory of Immunology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892
To investigate the role of protein tyrosine phosphatases in
IL-4R
-chain expression and signaling, we first established that
SHP-1, but not SHP-2, coimmunoprecipitated with anti-IL-4R chain
Abs in extracts prepared from resting lymphocytes. We further observed
that the protein tyrosine phosphatase inhibitors
Na3VO4 and pervanadate
blocked the striking induction of IL-4R-chain expression that is
mediated by IL-4. However,
Na3VO4 did not diminish
IL-4-induced Stat6 phosphorylation nor did it block the IL-4-mediated
increase in IL-4R-chain mRNA. The striking inhibition in total
cellular IL-4R-chain and in cell surface IL-4 receptors was
associated with an inhibition of biosynthetic labeling of
IL-4R-chain after a 30- min pulse with [35S]
methionine, indicating that reduction of IL-4R-chain protein
resulted from either a diminished production of the receptor or a rapid
degradation, possibly as a result of phosphorylation of the receptor in
an early biosynthetic cellular compartment. Control of newly
synthesized IL-4R-chain protein expression by phosphatase may
provide a novel means to regulate IL-4 responsiveness.
This article has been cited by other articles:
|
|
 |
|
  P. Pouliot, P. Camateros, D. Radzioch, B. N. Lambrecht, and M. Olivier Protein Tyrosine Phosphatases Regulate Asthma Development in a Murine Asthma Model J. Immunol., February 1, 2009; 182(3): 1334 - 1340. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Wang, R. Alvarez, G. Roderiquez, E. Guan, Q. Caldwell, J. Wang, M. Phelan, and M. A. Norcross CpG-Independent Synergistic Induction of {beta}-Chemokines and a Dendritic Cell Phenotype by Orthophosphorothioate Oligodeoxynucleotides and Granulocyte-Macrophage Colony-Stimulating Factor in Elutriated Human Primary Monocytes J. Immunol., May 15, 2005; 174(10): 6113 - 6121. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X. Lu, H. Nechushtan, F. Ding, M. F. Rosado, R. Singal, A. A. Alizadeh, and I. S. Lossos Distinct IL-4-induced gene expression, proliferation, and intracellular signaling in germinal center B-cell-like and activated B-cell-like diffuse large-cell lymphomas Blood, April 1, 2005; 105(7): 2924 - 2932. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z. Huang, J. Xin, J. Coleman, and H. Huang IFN-{gamma} Suppresses STAT6 Phosphorylation by Inhibiting Its Recruitment to the IL-4 Receptor J. Immunol., February 1, 2005; 174(3): 1332 - 1337. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W.-M. Yu, S. Wang, A. D. Keegan, M. S. Williams, and C.-K. Qu Abnormal Th1 Cell Differentiation and IFN-{gamma} Production in T Lymphocytes from Motheaten Viable Mice Mutant for Src Homology 2 Domain-Containing Protein Tyrosine Phosphatase-1 J. Immunol., January 15, 2005; 174(2): 1013 - 1019. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Zamorano, M. D. Rivas, A. Garcia-Trinidad, C.-K. Qu, and A. D. Keegan Phosphatidylcholine-Specific Phospholipase C Activity Is Necessary for the Activation of STAT6 J. Immunol., October 15, 2003; 171(8): 4203 - 4209. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. M. Hanson, H. Dickensheets, C.-K. Qu, R. P. Donnelly, and A. D. Keegan Regulation of the Dephosphorylation of Stat6. PARTICIPATION OF TYR-713 IN THE INTERLEUKIN-4 RECEPTOR alpha , THE TYROSINE PHOSPHATASE SHP-1, AND THE PROTEASOME J. Biol. Chem., January 31, 2003; 278(6): 3903 - 3911. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. J. Hirst, M. P. Hallsworth, Q. Peng, and T. H. Lee Selective Induction of Eotaxin Release by Interleukin-13 or Interleukin-4 in Human Airway Smooth Muscle Cells Is Synergistic with Interleukin-1beta and Is Mediated by the Interleukin-4 Receptor alpha -Chain Am. J. Respir. Crit. Care Med., April 15, 2002; 165(8): 1161 - 1171. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Perez-G., M. Melo, A. D. Keegan, and J. Zamorano Aspirin and Salicylates Inhibit the IL-4- and IL-13-Induced Activation of STAT6 J. Immunol., February 1, 2002; 168(3): 1428 - 1434. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. L. Wurster, D. J. Withers, T. Uchida, M. F. White, and M. J. Grusby Stat6 and IRS-2 Cooperate in Interleukin 4 (IL-4)-Induced Proliferation and Differentiation but Are Dispensable for IL-4-Dependent Rescue from Apoptosis Mol. Cell. Biol., January 1, 2002; 22(1): 117 - 126. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Kashiwada, C. C. Giallourakis, P.-Y. Pan, and P. B. Rothman Immunoreceptor Tyrosine-Based Inhibitory Motif of the IL-4 Receptor Associates with SH2-Containing Phosphatases and Regulates IL-4-Induced Proliferation J. Immunol., December 1, 2001; 167(11): 6382 - 6387. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. D. White, R. P. Andrews, and G. K. K. Hershey Sulfhydryl-2 domain-containing protein tyrosine phosphatase-1 is not a negative regulator of interleukin-4 signaling in murine mast cells J. Leukoc. Biol., May 1, 2001; 69(5): 825 - 830. [Abstract] [Full Text]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
