The JI
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     
 

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Munshi, N.
Right arrow Articles by Ganju, R. K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Munshi, N.
Right arrow Articles by Ganju, R. K.
Right arrowPubmed/NCBI databases
*Compound via MeSH
*Substance via MeSH
Medline Plus Health Information
*Kaposi's Sarcoma
Hazardous Substances DB
*L-TYROSINE
The Journal of Immunology, 2000, 164: 1169-1174.
Copyright © 2000 by The American Association of Immunologists

c-Src Mediates Mitogenic Signals and Associates with Cytoskeletal Proteins upon Vascular Endothelial Growth Factor Stimulation in Kaposi’s Sarcoma Cells1

Neru Munshi*, Jerome E. Groopman*, Parkash S. Gill{dagger} and Ramesh K. Ganju2,*

* Robert Mapplethorpe Laboratory, Divisions of Experimental Medicine and Hematology/Oncology, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02115; and {dagger} Division of Hematology/Oncology, Norris Cancer Center, University of Southern California, Los Angeles, CA 90033

Vascular endothelial growth factor (VEGF) appears to be a critical cytokine modulating the growth and spread of Kaposi’s sarcoma (KS). Furthermore, infection with the KS herpes virus results in up-regulation of VEGF and triggering of VEGF receptor activation. The molecular mechanisms regulating such cytokine-driven proliferation of KS cells are not well characterized. We investigated the role of Src-related tyrosine kinases in VEGF-mediated signaling in model KS 38 tumor cells. VEGF stimulation specifically activated c-Src kinase activity but not that of other related Src kinases such as Lyn, Fyn, or Hck in KS cells. Pyrazolopyrimidine, a selective inhibitor of Src family tyrosine kinases, significantly blocked the VEGF-induced growth of KS cells. Further studies using mutants of c-Src kinase revealed that Src mediates mitogen-activated protein kinase activation induced by VEGF. We also observed that VEGF stimulation resulted in increased tyrosine phosphorylation of the focal adhesion components paxillin and p130cas. Furthermore, VEGF induction enhanced the complex formation between Src kinase and paxillin. Src kinase appears to play an important functional role in VEGF-induced signaling in KS cells and may act to link pathways from the VEGF receptor to mitogen-activated protein kinase and cytoskeletal components, thereby effecting tumor proliferation and migration.




This article has been cited by other articles:


Home page
 IOVSHome page
W. Chen, D. B. Jump, W. J. Esselman, and J. V. Busik
Inhibition of Cytokine Signaling in Human Retinal Endothelial Cells through Modification of Caveolae/Lipid Rafts by Docosahexaenoic Acid
Invest. Ophthalmol. Vis. Sci., January 1, 2007; 48(1): 18 - 26.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
Y. Wu, S. Singh, M.-M. Georgescu, and R. B. Birge
A role for Mer tyrosine kinase in {alpha}v{beta}5 integrin-mediated phagocytosis of apoptotic cells
J. Cell Sci., February 1, 2005; 118(3): 539 - 553.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
Y. S. Cho, V. M. Chennathukuzhi, M. A. Handel, J. Eppig, and N. B. Hecht
The Relative Levels of Translin-associated Factor X (TRAX) and Testis Brain RNA-binding Protein Determine Their Nucleocytoplasmic Distribution in Male Germ Cells
J. Biol. Chem., July 23, 2004; 279(30): 31514 - 31523.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
K. Henriksen, M. Karsdal, J.-M. Delaisse, and M. T. Engsig
RANKL and Vascular Endothelial Growth Factor (VEGF) Induce Osteoclast Chemotaxis through an ERK1/2-dependent Mechanism
J. Biol. Chem., December 5, 2003; 278(49): 48745 - 48753.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
T. Matsumoto, J. Jiang, K. Kiguchi, L. Ruffino, S. Carbajal, L. Beltran, D. K. Bol, M. P. Rosenberg, and J. DiGiovanni
Targeted Expression of c-Src in Epidermal Basal Cells Leads to Enhanced Skin Tumor Promotion, Malignant Progression, and Metastasis
Cancer Res., August 15, 2003; 63(16): 4819 - 4828.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. Z. Fernandis, R. P. Cherla, and R. K. Ganju
Differential Regulation of CXCR4-mediated T-cell Chemotaxis and Mitogen-activated Protein Kinase Activation by the Membrane Tyrosine Phosphatase, CD45
J. Biol. Chem., March 7, 2003; 278(11): 9536 - 9543.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
Y. Aoki, M. Narazaki, T. Kishimoto, and G. Tosato
Receptor engagement by viral interleukin-6 encoded by Kaposi sarcoma-associated herpesvirus
Blood, November 15, 2001; 98(10): 3042 - 3049.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
Y. Aoki, R. Yarchoan, K. Wyvill, S.-i. Okamoto, R. F. Little, and G. Tosato
Detection of viral interleukin-6 in Kaposi sarcoma-associated herpesvirus-linked disorders
Blood, April 1, 2001; 97(7): 2173 - 2176.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Website Copyright © 2000 by The American Association of Immunologists, Inc. All rights reserved.
All Contents Copyright © 2000 by The American Association of Immunologists, Inc. All rights reserved.