| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
RIIIB-to-CR3 Proximity on Neutrophils1
*
Department of Biological Sciences, Wayne State University, Detroit, MI 48202; and
Howard Hughes Medical Institute and Department of Internal Medicine and Biological Chemistry, and
Department of Internal Medicine, University of Michigan School of Medicine, Ann Arbor, MI 48109
Previous studies have shown that Ebola virus’ secretory
glycoprotein (sGP) binds to Fc
RIIIB (CD16b) and inhibits L-selectin
shedding. In this study, we test the hypothesis that sGP interferes
with the physical linkage between CR3 and FcRIIIB. Neutrophils were
stained with rhodamine-conjugated anti-CD16b mAb (which does not
inhibit sGP binding) and fluorescein-conjugated anti-CR3 mAb
reagents and then incubated in media with or without sGP. Physical
proximity between fluorochrome-labeled CR3 and FcRIIIB on individual
cells was measured by resonance energy transfer (RET) imaging,
quantitative RET microfluorometry, and single-cell imaging
spectrophotometry. Cells incubated with control supernatants displayed
a significant RET signal, indicative of physical proximity (<7 nm)
between CR3 and FcRIIIB. In contrast, cells exposed to sGP showed a
significant reduction in the CR3-FcRIIIB RET signal using these
methods. Interestingly, colocalization and cocapping of CR3 and
FcRIIIB were not affected, suggesting that the proximity of these
two receptors is reduced without triggering dissociation. Thus, sGP
alters the physical linkage between FcRIIIB and
CR3.
This article has been cited by other articles:
|
|
 |
|
  V. M. Wahl-Jensen, T. A. Afanasieva, J. Seebach, U. Stroher, H. Feldmann, and H.-J. Schnittler Effects of Ebola Virus Glycoproteins on Endothelial Cell Activation and Barrier Function J. Virol., August 15, 2005; 79(16): 10442 - 10450. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Ortiz-Stern and C. Rosales Fc{gamma}RIIIB stimulation promotes {beta}1 integrin activation in human neutrophils J. Leukoc. Biol., May 1, 2005; 77(5): 787 - 799. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. J. Sullivan, M. Peterson, Z.-y. Yang, W.-p. Kong, H. Duckers, E. Nabel, and G. J. Nabel Ebola Virus Glycoprotein Toxicity Is Mediated by a Dynamin-Dependent Protein-Trafficking Pathway J. Virol., January 1, 2005; 79(1): 547 - 553. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. L. Kindzelskii, T. Ueki, H. Michibata, T. Chaiworapongsa, R. Romero, and H. R. Petty 6-Phosphogluconate Dehydrogenase and Glucose-6-Phosphate Dehydrogenase Form a Supramolecular Complex in Human Neutrophils That Undergoes Retrograde Trafficking during Pregnancy J. Immunol., May 15, 2004; 172(10): 6373 - 6381. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Sullivan, Z.-Y. Yang, and G. J. Nabel Ebola Virus Pathogenesis: Implications for Vaccines and Therapies J. Virol., September 15, 2003; 77(18): 9733 - 9737. [Full Text] [PDF]
|
|
|
|
 |
|
 V. V. Artym, A. L. Kindzelskii, W.-T. Chen, and H. R. Petty Molecular proximity of seprase and the urokinase-type plasminogen activator receptor on malignant melanoma cell membranes: dependence on {beta}1 integrins and the cytoskeleton Carcinogenesis, October 1, 2002; 23(10): 1593 - 1602. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Mahalingam, J. Meanger, P. S. Foster, and B. A. Lidbury The viral manipulation of the host cellular and immune environments to enhance propagation and survival: a focus on RNA viruses J. Leukoc. Biol., September 1, 2002; 72(3): 429 - 439. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Feldmann, V. E. Volchkov, V. A. Volchkova, U. Stroher, and H.-D. Klenk Biosynthesis and role of filoviral glycoproteins J. Gen. Virol., December 1, 2001; 82(12): 2839 - 2848. [Full Text] [PDF]
|
|
|
|
|
|
R. G. Sitrin, P. M. Pan, R. A. Blackwood, J. Huang, and H. R. Petty Cutting Edge: Evidence for a Signaling Partnership Between Urokinase Receptors (CD87) and L-Selectin (CD62L) in Human Polymorphonuclear Neutrophils J. Immunol., April 15, 2001; 166(8): 4822 - 4825. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. HORINO, A. L. KINDEZELSKII, V. M. ELNER, B. A. HUGHES, and H. R. PETTY Tumor cell invasion of model 3-dimensional matrices: demonstration of migratory pathways, collagen disruption, and intercellular cooperation FASEB J, April 1, 2001; 15(6): 932 - 939. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Q. Jiang, S. Akashi, K. Miyake, and H. R. Petty Cutting Edge: Lipopolysaccharide Induces Physical Proximity Between CD14 and Toll-Like Receptor 4 (TLR4) Prior to Nuclear Translocation of NF-{kappa}B J. Immunol., October 1, 2000; 165(7): 3541 - 3544. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
