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Enhancers Indicates That Different Elements Within the Enhancer May Mediate Activation of Transcription and Recombination1
Unité de Génétique et Biochimie du Développement, Unité de Recherche Associée 1960, Centre National de la Recherche Scientifique, Département d’Immunologie, Institut Pasteur, Paris, France
The intronic Ig 
-light chain enhancer (Ei) has been
implicated in regulation of transcription and V-J recombination
at the locus. To identify sequences within the Ei enhancer which
are involved in control of recombination, we have made use of the
finding that the Ei element from the rabbit b9 locus is capable
of inducing rearrangement, but not transcription of genes in mouse
lymphoid cells. We have therefore compared the binding of murine
nuclear proteins to the mouse and rabbit Ei elements. DNase I
footprinting and gel mobility shift assays indicate that only the B,
E1, and E2 sites of the rabbit enhancer are able to interact with
murine trans-acting factors. Moreover, although the
rabbit B site binds murine NF-B p50/p50 and p50/p65 complexes
with high affinity, this site is not capable of mediating
transcriptional activation of transient transfection reporter
constructs in mouse B lineage cells. These results therefore suggest
that, in contrast to the maintenance of enhancer transcription
which requires all of the Ei sites, only the B, E1, and E2
sites may be necessary for the recombinational activity of the
enhancer. Furthermore, NF-B-mediated effects on transcription and
recombination appear to involve separate downstream activation
pathways.
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