| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Departments of
*
Medicine,
Pathology, and
Microbiology and Molecular Genetics, Channing Laboratory, Brigham and Women’s Hospital, and
Division of Infectious Disease, Dana-Farber Cancer Institute, Harvard Medical School, Boston, MA 02115
The large-molecular-sized zwitterionic capsular polysaccharide of the anaerobe Bacteroides fragilis NCTC 9343, designated polysaccharide (PS) A, stimulates T cell proliferation in vitro and induces T cell-dependent protection against abscess formation in vivo. In the present study, we utilized a modification of a recently developed ozonolytic method for depolymerizing polysaccharides to examine the influence of the molecular size of PS A on cell-mediated immunity. Ozonolysis successfully depolymerized PS A into structurally intact fragments. PS A with average molecular sizes of 129.0 (native), 77.8, 46.9, and 17.1 kDa stimulated CD4+-cell proliferation in vitro to the same degree, whereas the 5.0-kDa fragment was much less stimulatory than the control 129.0-kDa PS A. Rats treated with 129.0-kDa, 46.9-kDa, and 17.1-kDa PS A molecules, but not those treated with the 5.0-kDa molecule, were protected against intraabdominal abscesses induced by challenge with viable B. fragilis. These results demonstrate that a zwitterionic polysaccharide as small as 22 repeating units (88 monosaccharides) elicits a T cell-dependent immune response. These findings clearly distinguish zwitterionic T cell-dependent polysaccharides from T cell-independent polysaccharides and give evidence of the existence of a novel mechanism for a polysaccharide-induced immune response.
This article has been cited by other articles:
|
|
 |
|
  S. Gallorini, F. Berti, G. Mancuso, R. Cozzi, M. Tortoli, G. Volpini, J. L. Telford, C. Beninati, D. Maione, and A. Wack Toll-like receptor 2 dependent immunogenicity of glycoconjugate vaccines containing chemically derived zwitterionic polysaccharides PNAS, October 13, 2009; 106(41): 17481 - 17486. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Groneck, D. Schrama, M. Fabri, T. L. Stephen, F. Harms, S. Meemboor, H. Hafke, M. Bessler, J. C. Becker, and W. M. Kalka-Moll Oligoclonal CD4+ T Cells Promote Host Memory Immune Responses to Zwitterionic Polysaccharide of Streptococcus pneumoniae Infect. Immun., September 1, 2009; 77(9): 3705 - 3712. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Moens, A. Jeurissen, S. Nierkens, L. Boon, L. Van Kaer, A. Kasran, G. Wuyts, J. L. Ceuppens, and X. Bossuyt Generation of Antibody Responses to Pneumococcal Capsular Polysaccharides Is Independent of CD1 Expression in Mice Infect. Immun., May 1, 2009; 77(5): 1976 - 1980. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Gallorini, F. Berti, P. Parente, R. Baronio, S. Aprea, U. D'Oro, M. Pizza, J. L. Telford, and A. Wack Introduction of Zwitterionic Motifs into Bacterial Polysaccharides Generates TLR2 Agonists Able to Activate APCs J. Immunol., December 15, 2007; 179(12): 8208 - 8215. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. S.C. Kreisman, J. H. Friedman, A. Neaga, and B. A. Cobb Structure and function relations with a T-cell-activating polysaccharide antigen using circular dichroism Glycobiology, January 1, 2007; 17(1): 46 - 55. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. L. Stephen, M. Niemeyer, A. O. Tzianabos, M. Kroenke, D. L. Kasper, and W. M. Kalka-Moll Effect of B7-2 and CD40 Signals from Activated Antigen-Presenting Cells on the Ability of Zwitterionic Polysaccharides To Induce T-Cell Stimulation Infect. Immun., April 1, 2005; 73(4): 2184 - 2189. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Stingele, B. Corthesy, N. Kusy, S. A. Porcelli, D. L. Kasper, and A. O. Tzianabos Zwitterionic Polysaccharides Stimulate T Cells with No Preferential V{beta} Usage and Promote Anergy, Resulting in Protection against Experimental Abscess Formation J. Immunol., February 1, 2004; 172(3): 1483 - 1490. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. M. Kalka-Moll, A. O. Tzianabos, P. W. Bryant, M. Niemeyer, H. L. Ploegh, and D. L. Kasper Zwitterionic Polysaccharides Stimulate T Cells by MHC Class II-Dependent Interactions J. Immunol., December 1, 2002; 169(11): 6149 - 6153. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. M. Kalka-Moll, Y. Wang, L. E. Comstock, S. E. Gonzalez, A. O. Tzianabos, and D. L. Kasper Immunochemical and Biological Characterization of Three Capsular Polysaccharides from a Single Bacteroides fragilis Strain Infect. Immun., April 1, 2001; 69(4): 2339 - 2344. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Wang, W. M. Kalka-Moll, M. H. Roehrl, and D. L. Kasper Structural basis of the abscess-modulating polysaccharide A2 from Bacteroides fragilis PNAS, December 5, 2000; 97(25): 13478 - 13483. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. O. Tzianabos, A. Chandraker, W. Kalka-Moll, F. Stingele, V. M. Dong, R. W. Finberg, R. Peach, and M. H. Sayegh Bacterial Pathogens Induce Abscess Formation by CD4+ T-Cell Activation via the CD28-B7-2 Costimulatory Pathway Infect. Immun., December 1, 2000; 68(12): 6650 - 6655. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
