HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Holz, A. Articles by Oldstone, M. B. A. Search for Related Content PubMed PubMed Citation Articles by Holz, A. Articles by Oldstone, M. B. A. Pubmed/NCBI databases Medline Plus Health Information Multiple Sclerosis

Myelin-Associated Oligodendrocytic Basic Protein: Identification of an Encephalitogenic Epitope and Association with Multiple Sclerosis¹

Andreas Holz^2,*, Bibiana Bielekova, Roland Martin and Michael B. A. Oldstone^*

^* Viral-Immunobiology Laboratory, Division of Virology, Department of Neuropharmacology, The Scripps Research Institute, La Jolla, CA 92037; and Neuroimmunology Branch, National Institute of Neurologic Disorders and Stroke, National Institutes of Health, Bethesda, MD 20892

Myelin-associated oligodendrocytic basic protein (MOBP) is an abundant myelin constituent expressed exclusively by oligodendrocytes, the myelin-forming cells of the CNS. We report that MOBP causes experimental allergic encephalomyelitis and is associated with multiple sclerosis. First, we note that purified recombinant MOBP inoculated into SJL/J mice produces CNS disease. Tests of overlapping peptides spanning the murine MOBP molecule map the encephalitogenic site to amino acids 37–60. MOBP-induced experimental allergic encephalomyelitis shows a severe clinical course and is characterized by a prominent CD4⁺ T lymphocyte infiltration and a lesser presence of CD8⁺ T cells and microglia/macrophages around vessels and in the white matter of the CNS. Second, PBL obtained from patients with relapsing/remitting multiple sclerosis mount a proliferative response to human MOBP, especially at amino acids 21–39. This response equals or exceeds the response to myelin basic protein and an influenza virus hemagglutinin peptide, both serving as internal controls. Thus, a novel myelin Ag, MOBP aa 37–60, plays a role in rodent autoimmune CNS disease, and its human MOBP counterpart is associated with the human demyelinating disease multiple sclerosis.

This article has been cited by other articles:

				J. M. Greer, P. A. Csurhes, D. M. Muller, and M. P. Pender Correlation of Blood T Cell and Antibody Reactivity to Myelin Proteins with HLA Type and Lesion Localization in Multiple Sclerosis J. Immunol., May 1, 2008; 180(9): 6402 - 6410. [Abstract] [Full Text] [PDF]

				N. Kaushansky, M.-C. Zhong, N. Kerlero de Rosbo, R. Hoeftberger, H. Lassmann, and A. Ben-Nun Epitope Specificity of Autoreactive T and B Cells Associated with Experimental Autoimmune Encephalomyelitis and Optic Neuritis Induced by Oligodendrocyte-Specific Protein in SJL/J Mice J. Immunol., November 15, 2006; 177(10): 7364 - 7376. [Abstract] [Full Text] [PDF]

				P. Fontoura, P. P. Ho, J. DeVoss, B. Zheng, B. J. Lee, B. A. Kidd, H. Garren, R. A. Sobel, W. H. Robinson, M. Tessier-Lavigne, et al. Immunity to the Extracellular Domain of Nogo-A Modulates Experimental Autoimmune Encephalomyelitis J. Immunol., December 1, 2004; 173(11): 6981 - 6992. [Abstract] [Full Text] [PDF]

				N. K. de Rosbo, J. F. Kaye, M. Eisenstein, I. Mendel, R. Hoeftberger, H. Lassmann, R. Milo, and A. Ben-Nun The Myelin-Associated Oligodendrocytic Basic Protein Region MOBP15-36 Encompasses the Immunodominant Major Encephalitogenic Epitope(s) for SJL/J Mice and Predicted Epitope(s) for Multiple Sclerosis-Associated HLA-DRB1*1501 J. Immunol., July 15, 2004; 173(2): 1426 - 1435. [Abstract] [Full Text] [PDF]

				M. P. Crawford, S. X. Yan, S. B. Ortega, R. S. Mehta, R. E. Hewitt, D. A. Price, P. Stastny, D. C. Douek, R. A. Koup, M. K. Racke, et al. High prevalence of autoreactive, neuroantigen-specific CD8+ T cells in multiple sclerosis revealed by novel flow cytometric assay Blood, June 1, 2004; 103(11): 4222 - 4231. [Abstract] [Full Text] [PDF]

				H. Lewicki, A. Tishon, D. Homann, H. Mazarguil, F. Laval, V. C. Asensio, I. L. Campbell, S. DeArmond, B. Coon, C. Teng, et al. T Cells Infiltrate the Brain in Murine and Human Transmissible Spongiform Encephalopathies J. Virol., March 15, 2003; 77(6): 3799 - 3808. [Abstract] [Full Text] [PDF]

				D. Jane-wit, M. Yu, A. E. Edling, S. Kataoka, J. M. Johnson, L. B. Stull, C. S. Moravec, and V. K. Tuohy A Novel Class II-Binding Motif Selects Peptides That Mediate Organ-Specific Autoimmune Disease in SWXJ, SJL/J, and SWR/J Mice J. Immunol., December 1, 2002; 169(11): 6507 - 6514. [Abstract] [Full Text] [PDF]

				J. M. Greer, B. Denis, R. A. Sobel, and E. Trifilieff Thiopalmitoylation of Myelin Proteolipid Protein Epitopes Enhances Immunogenicity and Encephalitogenicity J. Immunol., June 1, 2001; 166(11): 6907 - 6913. [Abstract] [Full Text] [PDF]

				M. P. Pender, P. A. Csurhes, J. M. Greer, P. D. Mowat, R. D. Henderson, K. D. Cameron, D. M. Purdie, P. A. McCombe, and M. F. Good Surges of Increased T Cell Reactivity to an Encephalitogenic Region of Myelin Proteolipid Protein Occur More Often in Patients with Multiple Sclerosis Than in Healthy Subjects J. Immunol., November 1, 2000; 165(9): 5322 - 5331. [Abstract] [Full Text] [PDF]