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V Repertoire During Thymic Ontogeny Suggests Three Novel Waves of TCR Expression

Department of Immunology, Paul Ehrlich Institute, Langen, Germany

Taking advantage of a PCR technique that allows amplification of all variable region genes with equal efficiency, we defined three novel waves of TCR -chain transcription during thymic ontogeny. The canonical DV101-D2-J2 rearrangement was confined to a narrow window from days 14 to 18 of gestation, indicating that the postulated two consecutive precursor waves bearing this canonical DV101 rearrangement will coincide on day 16. Neonatal -chain transcripts used a second wave of diverse V gene segments that are exclusively located in the locus-proximal gene cluster of intermingled single members of different V subfamilies. In the adult, only expression of a clan of three homologous subfamilies, ADV7, DV104, and ADV17, persists. The members of the ADV7 subfamily are also scattered across the locus, but their usage does not show the position-dependent bias of the other V-to- rearrangements.

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